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Isolation and Identification of Polyhydroxy Alkanoates Accumulating Bacteria from Sugar-Cane Industry Waste

Thesis Info

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Author

Farzana Yasmin

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=299

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721017746

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Polyhydroalkanoates (PHAs) are polyesters that are synthesized by different microorganism and stored as granules in microbes. They are used as a food under starvation. Studies have shown that physical properties of different PHAs resemble significantly to the petroleum based plastics and polyethylene. Additionally, microbial PHAs can be spun, mould and converted into filaments. On the other hand, PHAs are biodegradable material. Such promising features make PHAs as a potential candidate for next generation plastic as it will reduce the pollution caused by the petroleum based plastics. However, PHAs are costly as compared to the different petroleum based plastics. Therefore, researchers are trying to identify new microbes that may produce high amount of PHAs from waste carbon sources. They can accumulate high concentration of PHAs. During the crushing season sugar industries are producing tones of organic waste, specifically they are draining a considerable amount of sugar in canals. This can be a source of food for different types of bacteria, specifically for PHA accumulating bacteria. In this study, different PHAs accumulating bacteria were identified from the swage of sugar industry waste. Five soil and sewage samples were collected and stored at room temperature. For isolation and identification of PHAs accumulating bacteria, Samples were allowed to grow on LB agar medium plates supplemented with 1% Nile blue A. Different concentrations of Nile blue A were used for different incubation periods and colonies were screened. All these colonies were observed under ultraviolet light; PHAs accumulating bacteria were showed bright orange color inflorescence while non PHAs accumulating bacteria appeared white. The PHAs accumulating bacteria were selected and then amplified for 16S rRNA gene sequencing through PCR. In this study we have identified five PHAs accumulating bacteria from the waste of sugarcane industry by using the chemical and sequence analysis. These bacteria can be used for the laboratory and large scale production of different types of PHAs.
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فیض یابی

فیض یابی
۱۔ تفسیر القرآن الکریم۔ حافظ عبدالسلام
۲۔ مذاہب عالم کی آسمانی کتابیں۔غلام احمد پرویز
۳۔ تاریخ طبری۔علامہ طبری
۴۔ اساطیری تہذیبیں (تہران یونیورسٹی ایران)
۵۔ تاریخ ایران۔پروفیسر مقبول بیگ بدخشانی
۶۔ تاریخ فلسفہ اور ثقافتی نظام۔پنڈت سوامی رائے نظام آبادی
۷۔ زرتشت نامہ (تہران یونیورسٹی ایران)
۸۔ اردا ویراف نامہ۔ارتائی ویروف طبع تہران
۹۔ کنفیوشس ، زرتشت اور اسلام۔احمد دیدات
۱۰۔ ابتدائی فلسفہ۔ جان ہاسپرس
۱۱۔ مانویت (مانی مذہب) مسکونیہ ایرانی
۱۲۔ یونانی فلسفہ۔ڈبلیو۔ٹی۔سیٹس
۱۳۔ زیوس اور یونان۔ مائیکل ایلفرڈ
۱۴۔ ویراف سروش اور آذرایزد(ترجمہ)
۱۵۔ نشاط فلسفہ۔ول ڈیورا فٹ
۱۶۔ تہذیبی عجائب اور انسانی ارتقا۔ جان فرینکلین
۱۷ ایران کے قدیم ادبی شفاھی آثار
۱۸۔ ’’الکامل‘‘۔ ابن اثیر
۱۹۔ ’’البدیہ‘‘۔ابن کثیر
۲۰۔ بلیس ابلیس۔ علامہ ابن جوزی
۲۱۔ فلسفہ اور پران۔پنڈت شنکر اچاریہ
۲۲۔ عبرانی، سریانی مفکر۔ پروفیسر سلبر۔ تل ابیب یونیورسٹی۔
۲۳۔ تفسیر القرآن۔ علامہ طالب جوہری
۲۴۔ تفسیر القرآن۔ ڈاکٹر طاہر القادری
اس کے علاوہ زبور ، تورات، انجیل مقدس، صحائف لانبیائؑ

PENERAPAN PEMBELAJARAN KOOPERATIF MODEL GROUP INVESTIGATION (GI) UNTUK MENINGKATKAN MOTIVASI DAN HASIL BELAJAR BIOLOGI

Model pembelajaran mempengaruhi motivasi dan hasil belajar Biologi siswa sekolah menengah atas. Berdasarkan observasi awal, diketahui bahwa pembelajaran Biologi di kelas X-1 SMA Negeri 7 Malang memiliki beberapa ciri, yaitu (1) motivasi belajar siswa cenderung kurang, (2) dalam menyelesaikan suatu tugas, siswa dengan karakteristik yang sama cenderung mengelompok sehingga terbentuk kelompok homogen yang tidak seimbang di kelas, dan (3) metode ceramah yang diterapkan oleh guru tidak menarik minat siswa sehingga mempengaruhi hasil belajarnya. Berdasarkan kondisi tersebut maka dilakukan penelitian dengan menggunakan pembelajaran kooperatif model Group Investigation (GI) untuk meningkatkan motivasi dan hasil belajar Biologi siswa. Jenis penelitian ini adalah penelitian tindakan kelas dengan menggunakan pendekatan kualitatif dan dirancang dalam dua siklus. Hasil penelitian menunjukkan penerapan pembelajaran kooperatif model GI dapat meningkatkan motivasi dan hasil belajar Biologi siswa. Motivasi belajar siswa meningkat sebesar 31, 48% yaitu dari 46, 15% dengan kategori cukup pada siklus I menjadi 77, 63% dengan kategori baik pada siklus II. Hasil belajar siswa juga mengalami peningkatan, terlihat dari nilai rata-rata siswa sebesar 81, 94% pada siklus I menjadi 89, 18% pada siklus II dan siswa yang tuntas belajar juga mengalami peningkatan dari 79, 49% pada siklus I menjadi 97, 44 % pada siklus II. Hasil angket juga menunjukkan bahwa siswa menyukai dan lebih termotivasi untuk belajar Biologi setelah mengikuti pembelajaran dengan pembelajaran kooperatif model GI.

Bitrophic Effects of Artificial Diets of American Bollworm Helicoverpa Armigera H. on Different Biological Aspects of Bracon Hebetor Say

Helicoverpa armigera (Hubner) is a notorious pest of various fields and horticultural crops worldwide. Insecticides application ultimately responsible for resistance development in H. armigera, unsafe for the environment and natural enemies of insect pests. To withstand the pesticides resistance, biological control successfully helps in reducing pest population. Bracon hebetor is a cosmopolitan species that have the ability to parasitize a wide range of lepidopteran and few coleopteran hosts. As H. armigera fed on wide host range so, quality and quantity of food nutrients provided at the time of Bracon hebetor larval development is a crucial step. The need of the current research project is to focus on which best suited semi-synthetic artificial diet of H. armigera; B. hebetor developmental parameters are good and further, how it can effectively be used under the scenario of ecological conditions after getting reared on efficient host for the control of H. armigera. So we can utilize B. hebetor as an efficient biological control agent for H. armigera management. Study objectives were to screen out the best suited artificial diet of H. armigera for the rearing of B. hebetor. Investigation of B. hebetor life history traits on most efficient hosts from the screened host species for the effective rearing of B. hebetor. Determination of the effect of temperature, relative humidity regimes and host larval densities on the life history traits of B. hebetor parasitizing H. armigera. B. hebetor parasitization, reproduction, development, fitness related traits and life table parameters were observed on different pyralids, noctuids, gelechiids and curculionids under laboratory conditions (25±2°C and 70±5%) in order to find out the most suitable host for the mass rearing and inundative release purposes to control H. armigera. Results indicated that among four semi-synthetic diets (gram, rice, wheat and corn) of H. armigera, gram flour mediated diet was responsible for good biological and developmental parameters of B. hebetor under laboratory conditions. It can be successfully used for the rearing of H. armigera as a host for good parasitism and development of B. hebetor. Though, H. armigera itself was not an excellent host for the continuous rearing of parasitoid therefore, seven different hosts (six from Lepidoptera and one Coleoptera) were utilized to screen out the suitable one. Results showed that the pyralids hosts (E. kuehniella, C. cephalonica vii and G. mellonella) gave the best results for daily fecundity, parasitoid survival to adulthood, development time and female biased sex ratio followed by H. armigera and the worst reproduction occurred on H. postica. Among the most efficient screened pyralid hosts, B. hebetor biological parameters and fitness related traits were excellent on E. kuehniella as compared to C. cephalonica. So production of good fitness traits (wing area, tibia size and dry mass), excellent biological parameters (good immature numbers and adults emergence), short egg-adult development time and changes in THCs provide a good means for the immature development of B. hebetor within the parasitized E. kuehniella for several days. Temperature relative humidity regimes and H. armigera larval densities significantly affect the biological and developmental parameters of B. hebetor. Excellent life history parameters of B. hebetor parasitizing gram diet fed H. armigera were observed at 25°C and 60% R.H. combination. Among various larval densities of H. armigera good biological parameters of B. hebetor like production of immature numbers, short egg-adult development time, survivorship and female progeny production was significantly good on five larval density of H. armigera offered for parasitization. Therefore, it is recommended that gram mediated diet is best suited for the rearing of H. armigera in order to find out the best suited temperature, humidity combination and host larval density in the field release planning and maintenance of B. hebetor population for long term field crops management to control H. armigera. On the other hand B. hebetor quality progeny production on E. kuehniella make this insect of great potential to serve as valuable material for laboratory rearing and inundative field release to control H. armigera larval populations.