مولانا عبدالمجید حریری مرحوم
دوسرا علمی حادثہ مولانا عبدالمجید حریری مرحوم کی وفات کا ہے، انھوں نے بھی گزشتہ مہینہ وفات پائی، مرحوم مدنپورہ بنارس کے ایک ممتاز انصاری خاندان سے تھے، عربی اور انگریزی دونوں زبانوں کے فاضل تھے، عربی کی تکمیل کے بعد انگریزی کی تعلیم علی گڑھ کالج میں حاصل کی تھی، اور اپنے دور کے ممتاز طلبہ میں تھے، یہیں سے خلافت اور نان کو اپریشن کی تحریک میں شریک ہوئے اور ایک زمانہ تک جنگ آزادی میں سرگرمی سے حصہ لیتے رہے، اس دور کے تمام بڑے بڑے لیڈروں سے ان کے تعلقات تھے، ہندوستان کی آزادی کے بعد حکومت ہند نے ان کو سعودی عرب میں قونصل جنرل مقرر کیا، کئی سال تک اس عہدہ پر رہے، اس سے سبکدوش ہونے کے بعد کچھ دنوں تک سعودی عرب کے پایہ تخت ریاض میں قیام رہا، مرحوم ہندوستان کے ممتاز صاحب علم تھے، عربی زبان پر ان کو اہل زبان کی جیسی قدرت حاصل تھی، مقرر بھی اچھے تھے، لیکن افسوس ان کے سیاسی ذوق نے ان کے علمی جوہروں کو چمکنے نہ دیا، ایک عرصہ سے اس کا دائرہ بھی مقامی سیاست تک محدود ہوگیا تھا، اس لئے وہ جس علمی شہرت کے مستحق تھے وہ ان کو حاصل نہ ہوسکی اور اب عرصہ سے خانہ نشینی اختیار کرلی تھی، وہ مسلکاً اہل حدیث تھے، لیکن ہر مسلک کے علماء اور اہل علم سے ان کے تعلقات تھے، دارالمصنفین کے بزرگوں سے بھی ان کے پرانے روابط تھے، اس سلسلہ میں کئی مرتبہ ان کا یہاں آنا ہوا گزشتہ سال ایک تقریب سے بنارس جانا ہوا تو ملاقات کے لئے ان کی خدمت میں بھی حاضر ہوا تھا، بہت ضعیف ہوچکے تھے دماغ بھی پوری طرح کام نہ دیتا تھا، تعارف کے بعد پہچانا اور بڑی شفقت و محبت سے پیش...
Background: Ethical and moral values form the basis of the environment in any academic institution. Objectives: This study aimed to explore the ethical and moral issues faced by medical students during their undergraduate medical education in Pakistan. Methods: This qualitative exploratory study was conducted at ABWA Medical College, Faisalabad. It included thirteen semi-structured, one-to-one interviews of volunteering undergraduate medical students of Punjab, Pakistan, from September 2021 to February 2022. The interview invitation was sent electronically to social media groups. Member checking of transcribed interviews was done, and data were analyzed thematically using Atlas-ti software by two independent researchers. Results: Thematic data analysis led to the formation of 28 codes, 13 subthemes, and 3 main themes. Based on the social constructive approach, three main themes were categorized into ethical issues in (1) academic, (2) social, and (3) clinical contexts. Conclusions: The results showed that undergraduate medical students face a myriad of ethical and moral disruptions during their undergraduate study period at medical college. Much awareness about identifying and dealing with such issues is recommended to bring more quality to the program.
Bioactive agents like secondary metabolites and peptides are gaining much interest while addressing the issues of agricultural and health threats including pests and pathogens and in drug development. Among the plant bioactive peptides, cyclotides being disulphide rich, stable, resistant and having ability to graft epitopes on it or allow sequence variations in its different loops that are very important regarding biological application and research interests for drug development and delivery. The present study was therefore focused on evaluating the bioactive potential of cyclotide bearing indigenous plants including Viola odorata, Viola tricolor, Viola hybrid, Petunia, Clitoria ternatea, pansy F1, Panicum vigatum, Panicum laxam, Panicum maximum and Hamelia patens. The extracts were prepared in phosphate buffer saline (PBS) and protein extraction buffer (PEB). Ptunia and MCOTI-I both separately showed highest DPPH activity (antioxidant) due toas the activity was reduced significantly after treating with Proteinase K. Hamelia possessed highest reducing power, hemolytic, thromobolytic and antimicrobial activites. Mutagenic responses of the (Ames test) medicinal plants were not significant. DNA demage protection assays were also done on all protein extracts including MCOTI-I. Cyclotide genes were also isolated from the selected plant’s DNA using specific primers. It was found that the chimeric arrangement of cliotide gene (from Clitoria plant) as most attractive with conserved sequences in cyclotide and Albumin-1 domains and little intronic variations. Moreover, for cyclotide-protein interaction studies, Texas Red-DBCO amine dye was synthesized to label MCOTI-I-AziF with p-azidophenylalanine Uaa in origami DE3. Optimizations were first done by using the MCOTI-I and its label form MCOTI-I-Lys-TxRd. The click reaction to bind TxRd-DBCO with MCOTI-I-AziF was finally done in buffered guianidinum HCl at a molar ratio of 1:100 and analysed through LC/MS and MCOTI-I-AziF-TxRd-DBCO was confirmed by mass spectrometry. For binding assay FRET analysis was done with Trypsin-EGFP, saturation was achieved with KD value 29.7 ± 1.08 nM. Optimization of same amount of MCOTI-I inteins (using westernblot) was taken in 3-4 h. The IPTG induction avoided the overnight incubations and unwanted backgrounds. The MCOTI-I a cyclotide was expressed for the first time in yeast cells. Current research opens new understandings towards the bioactive xii potential of cyclotide bearing plants with/without peptide content, genetics of cyclotides genes from indegenous plants, optimizations of expression and in vitro labeling studies of MCOTI-I for optical studies regarding drug development and targeting.