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Home > Analysis of Cytochrome Oxidase-I Gene in Tilapia Fish Species Tilapia Zillii

Analysis of Cytochrome Oxidase-I Gene in Tilapia Fish Species Tilapia Zillii

Thesis Info

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Author

Ufaq Pervaiz

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=305

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721018868

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DNA barcoding is a technique concerned with the classification of things that is done with the help of small gene or DNA sequence with a known location on a chromosome that can be used to identify individuals or species in organisms mitochondrial DNA (mt DNA) which is helpful in recognition of meticulous species. It uses sequence variety in a 658-base pair fragment near the 5? end of the mitochondrial cytochrome c oxidase subunit I (COI) gene as a means for species recognition. DNA barcoding is a more defined and steady method in comparison with the classification based on the form and structure of the organism. It is equally useful in any stage of life cycle of fishes. The present research project was designed to recognize tilapia fish species (Tilapia zillii) of Pakistan genetically. Blood samples (n=30) were collected from Tilapia fish and genomic DNA was extracted and confirmed by 1% agarose gel. A short segment of COI gene (680bp) was amplified. PCR products were sequenced and analyzed by bioinformatics tools. Number of the haplotypes was 7. The haplotype diversity was Hd: 0.584 while nucleotide diversity was Pi: 0.00244. The mean intraspecific K2P genetic distance was 0.019. The estimated transition/transversion bias R was 1.40 that showed that this species possess very low genetic diversity. COI may supply a landmark for the classification of associated species at molecular level. As tilapia is extensively used for food and many other purposes so use of DNA barcoding technique is very helpful in discriminating it from other correlated fish species. It will also reduce the chance of mislabeling of tilapia fish species during its trading internationally as well as the species assessment at national level.
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کلرک کا نغمہ محبت کا تنقیدی جائزہ

کلرک کا نغمہ محبت کا تنقیدی جائزہ
میرا جی کے سماجی شعور کااظہار ان کی کئی نظموں میں ہوا ہے۔ جہاں چھوٹے طبقوں سے ان کی واضح وابستگی کا اندازہ ہوتا ہے ان کی نظمم کلرک کانغمہ محبت اس کی بہترین مثال ہے۔
متوسط طبقے کی ذہنی عکاسی:
سب رات مری سپنوں میں گزر جاتی ہے اور میں سوچتا ہوں
پھر صبح کی دیوی آتی ہے
اپے بستر سے اٹھتا ہوںمنہ دھوتا ہوں
لایا تھا کل جو ڈبل روٹی
اس میں سے آدھی کھائی تھی
باقی جو بچی وہ میرا آج کا ناشتہ ہے
اس بندمیں جس طرح ایک متوسط طبقے کے شخص کی ذہنی عکاسی کی گئی ہے۔ وہ مشاہدے کی زیر کی دلیل ہے۔ متوسط طبقے کا یہ شخص کیسے ایسے سہانے خواب دیکھتا ہے لیکن حقیقت یہ ہے کہ رات کی بچی ہوئی روٹی کھا کر دفتر روانہ ہونا پڑتاہے۔ حقیقت اور سپنے کا یہ تضاد میر ا جی کے مشاہدے کی دلیل تو ہے ہی ‘ کلرک کے ساتھ ان کی وابستگی اور اس کے مسائل کو اسی کی سطح پر اتر کر سمجھنے کی ایک کوشش بھی ہے۔ اس نظم میں محرومیوں کا طویل سلسلہ ہے جن سے اس نظم کا مرکزی کردار گزرتاہے۔ ا? س پاس کی ا?سائشوں اور لوگوں کے اطمینان و سکون کو دیکھتا دفتر پہنچتا ہے۔ اور اپنے افسر کی فضول باتیں سنتا ہے۔ نظم کا اختتام ان سطروں پر ہوتا ہے۔
پل بھر کے لیے اپنے کمرے کو فائل لینے ا?تا ہوں
اور دل کی آگ سلگتی ہے میں بھی جو کوئی افسر ہوتا
اس شہر کی دھول اور گلیوں سے کچھ دورمرا گھر ہوتا
اور تو ہوتی
لیکن میں تو اک منشی ہوں تو اونچے گھر کی رانی ہے
یہ میری پریم کہانی ہے اور دھرت سے بھی پرانی ہے
طبقاتی ناانصافیوں کا نوحہ:
یہ ایک...

ابن تیمیہ متفرد کیوں؟ اسباب و علل کاتجزیاتی مطالعہ A Research Review of the Reasons of Ibn Taymiyya’s Distinctions

Ibn Taymiyya is known as a controversial figure due to his differences. Most of the scholars have differed with him on most of the jurisprudential and principled issues. There are many reasons for Ibn Tamiya’s differences. The difference between intellect and its use is the most important, that is, the way of thinking. The jurisprudential ability and competence that elevated him to the status of ijtihad was a result of uniqueness in ijtihad and jurisprudence. Disagreements can be caused by the circumstances of that era and the behavior of the people of that era. All the principles and their preferred methods based on which he solved jurisprudential and doctrinal issues and all the reasons why he disagreed with a section of the ummah and the ummah know these differences in the form of the differences of Ibn Taymiyya. It is very important that his jurisprudential insight and ijtihad efforts be revealed, although in many issues the majority of the scholars of the ummah have disagreed with him and their opinion differs from the great taste. Ibn Tamiya’s method of inference in jurisprudential and principled issues and the principles by which he formed an opinion on an issue and on what basis he preferred principles in solving problems are the key issues that will be discussed in the following article.

Pomegranate Peel Based Novel Food Product

Pomegranate peel (PoP) is a valuable waste of juice processing industry and is a rich source of biomolecules of immense health significance. Plentiful research based evidences on ethnopharmacological and nutraceutical properties of PoP calls for the attention of food scientists in designing food products with innate ability to improve quality of life. This study focuses on exploring nutritional and nutraceutical properties of PoP, hydro-alcoholic extracts and the bagasse as ingredient of choice in developing novel food product. An indigenous cultivar of pomegranate “Alipuri” was procured from the local farm of Southern Punjab and pomegranate peel hydro-alcoholic extracts (PoPx) were derived from the peel fraction of the fruit. Nutritional and biological properties of PoP revealed the waste as a potential source of compounds bearing significant antimicrobial, antioxidant and antiulcer activities. Hydro-alcoholic extracts of PoP revealed significantly (p<0.05) higher concentration of phenolics i.e. 427.19 mgGAE/g and radicals scavenging activities as measured by DPPH and FRAP assays. Hydro-alcoholic extracts of PoP inhibited urease activity by 97.9% and IC50 for different extracts was recorded ~44.4 μM. PoP extracts presented significant inhibitory properties against gram positive and negative microorganisms including Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium, Bacillus subtilis, Staphylococcus aureus and Aspergillus niger. Minimum inhibitory concentration (MIC) of PoPx was recorded between 0.25–0.89 mg/mL. With reference to its features to be utilized as a food ingredient, PoP and the peel bagasse (PM) were incorporated in baked good for the development of nutritionally enriched and biologically stable cookies. The study revealed PoP supplementation to impart significant (p<0.05) improvement in dietary fibers (0.32–1.96 g/100 g), total phenols (90.7–161.9mg GAE/100 g) and inorganic residues (0.53–0.76 g/100 g) of cookies. Cookies carrying highest levels of PoP (7.5%), PoPx (1.0%) and PM (7.5%) were recorded with more than 50% radical scavenging activity whereas, products phenolics pool significantly inhibited oxidative and microbiological deterioration of the finished good during three months storage period. Cookies supplemented with PoP were recorded to present organoleptically acceptable quality at 6.0% supplementation while least difference in sensorial profile was noticed among control and cookies developed with 1.0% PoPx. Toxicity assessment of PoPx at higher doses i.e. 1000ppm presented non-lethal response in brine shrimps larvae. Safety assessment of PoP, PoPx and PM revealed non-significant (p>0.05) 8 effect of supplementation on various blood indices among normal healthy rats model. Lipid modulatory role of PoP and its extracts was witnessed convincingly indicating PoP and the extracts as viable ingredient in improving high density lipoprotein and lowering low density lipoprotein concentration. The study delineates no abnormal rise or fall in hematological parameters thereby suggesting a nontoxic response of PoP and its various fractions. The findings of this research propose PoP as a valuable waste of food processing industry that could further be exploited in designing nourishing recipes bearing disease preventive and ameliorative features.