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Home > Antimicrobial and Antifungal Effect of Ginger Zingiber Officinale , Green Tea Camellian Sinensis and Neem Azadirachta Indica on Selective Bacterial and Fungal Pathogensof Crop Plants: A Comparative Study

Antimicrobial and Antifungal Effect of Ginger Zingiber Officinale , Green Tea Camellian Sinensis and Neem Azadirachta Indica on Selective Bacterial and Fungal Pathogensof Crop Plants: A Comparative Study

Thesis Info

Access Option

External Link

Author

Salman Shahid

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=309

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721020516

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Plants have been a source of food, medicines and many other daily life products since primitive times. Bacterial and fungal pathogen attack reduces crop yield. Phytochemicals as biocides can kill microbes. In this study extract of Zingiber officinale rhizomes (Ginger), leaves of Azadirachta indica (Neem) and Camellian sinensis (Green tea) applied on bacterial strains, Xanthomonas syringae, and fungal pathogen, Alternaria alternata, to check their antibacterial and antifungal activity respectively. Ethanolic and aqueous extracts were prepared. Ethanolic and aqueous extracts showed a different yield. The bacterial pathogen was grown on ?Nutrient Agar media (NA)? and the fungal pathogen was grown on ?Potato Dextrose Agar media (PDA)?. Antibacterial and antifungal potential of plant extracts were observed at two different stages (at the time of inoculation and after growth of pathogen on media). The method used for assessing the inhibition zone was ?Agar Well Diffusion method?. MIC was also measured by ?Micro Broth Dilution Method?. Ethanolic extract of green tea showed the highest inhibition zone on Xanthomonas compestris was 12.5?1.0mm while lowest inhibition zone of 6.0?0.5mm was due to the aqueous extract of green tea. Antimicrobial activity of other extracts was in between these two ranges. Antibacterial and antifungal activity of ethanolic extract of ginger rhizome and neem leaves was 11.5?1.0, 6.0?1.0, 11.5?1.0, and 7.5?0.5, respectively. Aqueous extract of ginger rhizome showed no antifungal activity while antibacterial activity was 3.0?0.5mm. The lowest antibacterial activity was shown by the ethanolic extract of neem and the lowest antifungal activity was also due to the aqueous extract of neem with the inhibition zone of 0.8?1.0 and 1.0?0.5mm, respectively. Highest antibacterial and antifungal activity were shown by aqueous extract of green tea leaves with the inhibition zone of 12.5?1.0 and 3.0?0.6mm, respectively. MIC values of aqueous extract of ginger rhizome and green tea leaves were 158.3mg/ml for Alternaria alternata, which is the highest MIC for the fungal pathogen. However, the lowest MIC value for Alternaria alternata was 75mg/ml by ethanolic extract of neem leaves. The others extract showed antifungal MIC value in between these two ranges. Findings of the study will help in agriculture disease control management on pathogens of wheat such as X. syringae, and A. alternata. Advance researches are obligatory to explore interaction and microbial growth inhibition mechanisms of divergent phytochemicals from different plants. The awareness of the effectiveness of pooled extracts can be expanded from food implementations to the pharmacological and chemical field.
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والی آسی

والیؔ آسی
۱۴؍ اپریل کو اردو کے ایک خوش گو اور خوش فکر شاعر والی آسی کا انتقال ہوگیا، انہیں شاعری اور اردو زبان کی خدمت کا ولولہ اپنے نامور والد، مولانا عبدالباری آسی مرحوم سے وارثت میں ملا تھا۔ والی مرحوم کی تعلیم ممتاز اسکول اور امیر الدولہ اسلامیہ کالج لکھنؤ میں ہوئی تھی۔ جناب ساجد لکھنوی سے ان کی دوستی تھی، دونوں نے لاٹوش روڈ پر مکتبہ دین و دنیا قائم کیا، جہاں سے متعدد مجموعے شائع کئے، دونوں نے مل کر نعتیہ کلام کا ایک مجموعہ " ارمغانِ نعت" کے نام سے مرتب کر کے شائع کیا تھا، جو بہت مقبول ہوا۔
والی مرحوم شریف، ملنسار مگر خود دار شخص تھے، صوم و صلوٰۃ کے پابند تھے ان کی شاعری فکر و خیال کی مہارت اور اسلامی رنگ کی حامل ہوتی تھی، ان کے دو مجموعے ’’شہد‘‘ اور ’’موم‘‘ کے نام سے شائع ہوچکے ہیں، رسالوں میں بھی ان کا کلام چھپتا رہتا تھا، مشاعروں میں بھی شریک ہوتے تھے، ان کا ترنم بہت اچھا تھا مگر اکثر تحت اللفظ پڑھتے تھے، آواز اتنی پاٹ دار ہوتی تھی کہ سامعین خود بہ خود متوجہ ہوجاتے تھے، اپنے جان دار کلام کی وجہ سے ملک کے علاوہ دوحہ، قطر، مسقط، جدہ، دبئی، لندن، اور نیویارک کے مشاعروں میں بھی مدعو کئے جاتے تھے، ان کے شاگردوں کا وسیع حلقہ تھا، جن میں مشہور شاعر منور رانا بھی ہیں، وہ بڑے یار باش تھے، امین آباد میں ان کی دکان پر دوست شاعروں کا جمگھٹ رہتا تھا۔ ان کے چند اشعار ملاحظہ ہوں، جو آزادی کے پچاس ۵۰ برس گزرنے کی مناسبت سے کہے گئے ہیں:
کبھی کیا نہ کسی سے بیاں پچاس برس
کہ ہم نے کیسے گزارے یہاں پچاس برس
یہ چاہتے تھے قصیدہ ترا لکھیں لیکن
لہو میں ڈوبی رہیں انگلیاں پچاس...

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