Induced spawning has gained much importance due to the increasing demand of fish seed. The seed gathered from rivers does not fulfill the requirements of the aquaculture sector. The effects of induced spawning are under discussion because utilization of Ovaprim, a synthetic hormone also raises the question of fish health. The present research was conducted to know the effects of induced spawning on hormonal, histological and spermatological parameters of Indian major carps. The results depict that Ovaprim administration result in abrupt change in testosterone, LH and FSH level in Indian major carps. The change in LH, FSH and testosterone level is not permanent and it decreases after the induced spawning. After induced spawning fish weight also decreases that is due to the release of milt and egg. The weight change also depicts the weight of milt and eggs released. Histological examination exposed Necrosis and infiltration of mononuclear cells in liver of males of Indian major carps. Another change in liver of male fishes is detachment of hepatocytes from one another. Major changes that occurred in liver of Indian major carps (Female) include cellular swelling and fatty acid changes in females. The histological examination of kidney of male fish showed clear atrophy in glomerulus. Moreover interstitial nephritis was very much evident. In addition infiltration of lymphocytes was also observed with macro phages. Interstitial nephritis and other changes that were found in kidney cells of fish (male) include permeation of mono nuclear cells & shedding of tubular cells. Histological examination of male gonads showed Lobules of testis having residual spermatids in Labeo rohita. In case of Cirrhinus Mrigala testis were observed with active stage of spermatogenesis. Lobules were found to be packed with highly active spermatids in Catla catla. Female Fish gonads were observed normal. There were found diverse phases of follicles in Catla catla. Fish Ovary was found to be with immature follicles and germinal epithelium and presence in Cirrhinus mrigala and Labeo rohita. Spermatological parameters observed showed increased sperm density, sperm motility duration and sperm motility percentage after administration of Ovaprim. Moreover percentage of live and dead sperm showed that there was increase in live sperm percentage in fishes administered with Ovaprim. So, it is concluded that induced spawning influence the fish health in many ways including the percentage of sperm motility and sperm density. Key words: induced spawning, spermatology, hormonal changes, FSH, LH, Testosterone