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Effect of Induced Spawningon Hormonal, Histological and Spermatologicalchanges in Indian Major Carps

Thesis Info

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External Link

Author

Saeed Akram Khan

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=311

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721021143

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Induced spawning has gained much importance due to the increasing demand of fish seed. The seed gathered from rivers does not fulfill the requirements of the aquaculture sector. The effects of induced spawning are under discussion because utilization of Ovaprim, a synthetic hormone also raises the question of fish health. The present research was conducted to know the effects of induced spawning on hormonal, histological and spermatological parameters of Indian major carps. The results depict that Ovaprim administration result in abrupt change in testosterone, LH and FSH level in Indian major carps. The change in LH, FSH and testosterone level is not permanent and it decreases after the induced spawning. After induced spawning fish weight also decreases that is due to the release of milt and egg. The weight change also depicts the weight of milt and eggs released. Histological examination exposed Necrosis and infiltration of mononuclear cells in liver of males of Indian major carps. Another change in liver of male fishes is detachment of hepatocytes from one another. Major changes that occurred in liver of Indian major carps (Female) include cellular swelling and fatty acid changes in females. The histological examination of kidney of male fish showed clear atrophy in glomerulus. Moreover interstitial nephritis was very much evident. In addition infiltration of lymphocytes was also observed with macro phages. Interstitial nephritis and other changes that were found in kidney cells of fish (male) include permeation of mono nuclear cells & shedding of tubular cells. Histological examination of male gonads showed Lobules of testis having residual spermatids in Labeo rohita. In case of Cirrhinus Mrigala testis were observed with active stage of spermatogenesis. Lobules were found to be packed with highly active spermatids in Catla catla. Female Fish gonads were observed normal. There were found diverse phases of follicles in Catla catla. Fish Ovary was found to be with immature follicles and germinal epithelium and presence in Cirrhinus mrigala and Labeo rohita. Spermatological parameters observed showed increased sperm density, sperm motility duration and sperm motility percentage after administration of Ovaprim. Moreover percentage of live and dead sperm showed that there was increase in live sperm percentage in fishes administered with Ovaprim. So, it is concluded that induced spawning influence the fish health in many ways including the percentage of sperm motility and sperm density. Key words: induced spawning, spermatology, hormonal changes, FSH, LH, Testosterone
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یہ ہنر آزما کے دیکھتے ہیں

یہ ہنر آزما کے دیکھتے ہیں
اب کے اُن کو بھلا کے دیکھتے ہیں

رخ سے سرکے کبھی جو آنچل، ہم
معجزے اُس خدا کے دیکھتے ہیں

اب کے ہم بھی تو بے وفا ہو کر
جوش اُس کی وفا کے دیکھتے ہیں

کوئی اپنا نکل بھی سکتا ہے
ہاتھ سب سے ملا کے دیکھتے ہیں

درد میں آئے کچھ کمی شاید
آس کا گھر جلا کے دیکھتے ہیں

مل ہی جائے گا شعلۂ امید
گردِ اِیذا ہٹا کے دیکھتے ہیں

یاروں کی کھوجتے ہیں اب نیت
اپنا مقتل سجا کے دیکھتے ہیں

جو بھی ہوتا ہے خوش یہاں اُس کو
درد سے، منہ اُٹھا کے دیکھتے ہیں

جو بھی آتا ہے اُس گلی سے ہم
پاس اُس کو بٹھا کے دیکھتے ہیں

Development of Kabul under Mughals 1504-1738 AD

Kabul was a bridge between Indian Mughal Empire and Central Asia, the ancestral homeland of the founder of the Mughal Empire. Kabul, the capital of Afghanistan, carries about 3,500 years old historical records mentioning Kabul with different names like, Kubha, Gandahara, Kabura, Ortospana, Kapul, Zavul, and Zabul etc. Many great warriors and conquerors from Central Asia used Kabul as their route to India. It was also known as a gateway to India and Central Asia. Kabul became the foundation stone for the Mughal Empire in India. During the Mughal era Kabul entered into a new phase and with the invasion of Babur the area got the position of the capital of the Mughals. The early Mughal rulers paid much attention to the affairs of Kabul, because their existence to a greater extent was dependent on their strong hold over Kabul. The research work is focused on development of Kabul under Mughal kings particularly Babur, Humayun, Akbar, Shah Jahan and Aurangzeb.  The later Mughal kings after Aurangzeb were not able to end political disturbances in Kabul which not only displeased the people of Kabul but also encouraged the neighbouring powers to invade the valley. Historical and analytical methods are used in this research and Pashtu, Dari, Urdu and English sources have been utilized to gauge development of Kabul during that point in time. No research work has been carried out on this aspect of Kabul so far. The analysis of the development of Kabul under Mughals brings to limelight the geostrategic and politico-economic worth of Kabul as an important Caravanserai on the trade route between Central and South Asia.

Genetic Basis of Variation for High Temperature Tolerance in Gossypium Hirsutum L.

Cotton is the leading fiber crop in Pakistan. Its production is negatively affected by living (biotic) and non-living (abiotic) factors and among those, high temperature is most alarming one. Present study was conducted to explore the genetic basis of heat tolerance in upland cotton. Fifty upland cotton genotypes were screened against high temperature on the basis of CMT%, canopy temperature, seed cotton yield, node number of first fruiting branch, days to first effective boll, heat index (HI) and heat tolerance index (HTI).Significant variation was observed among fifty genotypes on the basis of traits used for screening. Seven heat tolerant (CIM-602, Cyto-178, CIM-616, FH-113, CIM-600, FH-114 and KZ-189) and 5 heat sensitive (AA-802, ARK-3, AGC-501, KZ-191 and VH-389) genotypes were selected for crossing. Selected parents were crossed in North Carolina II mating design fashion in the glasshouse. Seven parent and thirty five F1 crosses were field planted under normal and heat stress condition. The analysis of North Carolina Design II revealed that significant genotypic variations exist in all characters under study like plant height, number of sympodial branches per plant, number of monopodial branches per plant, number of bolls per plant, boll weight, GOT%, seed cotton yield, fiber length, fiber strength, fiber fineness, CMT%, node number of first fruiting branch and days to first effective boll. High differences were observed between phenotypic and genotypic variances for various traits which depicted high environmental effect on all studied traits. Among testers AGC-501, AA-802 and VH-389 were found best male combiners for most of the traits. FH-114 showed good GCA effects for plant height and bolls per plant, Cyto-178 for monopodial branches per plant and boll weight, FH-113 for most of the traits studied both under normal and stressed conditions. The crosses FH-114 × ARK-3 and FH-114× AGC-501 showed good SCA effects for most of the traits. Selecting such crosses would be beneficial for the development of superior hybrid through heterosis breeding. Crosses that showed significantbetter parent heterosis for different traits were FH-114 × ARK-3 for plant height,Cyto-178 × AA-802 for monopodia,CIM-616 × ARK-3 for sympodial branches, FH-114 × VH-389 for number of bolls, FH-113 × AA-802 for boll weight, Cyto-178 × KZ-191 for seed cotton yield, CIM-616 × KZ-191 showed maximum fiber length, FH-114 × ARK-3 for fiber strength, FH-114 x KZ-191 for fiber fineness, Cyto-178 × AGC-501 for GOT%, CIM-616 x AA-802 for canopy temperature, FH-114 × VH-389 for node number of first fruiting branch, FH-114 x AA-802 for days to 1st effective boll and CIM-616 × VH-389 for CMT %.As most of the traits were governed by non-additive genes with low narrow sense heritability, so it is suggested that selection may be delayed, or development of hybrid variety would be more effective under high temperature areas.