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Home > Micro-Rna268 over Expression Affects Rice Seedling Growth under Zinc Deficiency

Micro-Rna268 over Expression Affects Rice Seedling Growth under Zinc Deficiency

Thesis Info

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Author

Sobia Shafqat

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=313

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721021509

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In plants and animal, role of RNAs (miRNAs are 21-24-nucleotide-long) as wherever post-transcriptional screen appearance of gene. Response to abiotic and biotic stressor, rising evidence focuses toward the essential position of miRNAs in plant. Zn is exceptionally harmless to plants and basic heavy metal. Some substantial metals are generally harmless, (for example,ruthenium, silver, and indium), or either fundamental supplements (normally iron, cobalt, and zinc), however in larger quantity or certain structures it is very well may be poisonous. Other important metals are deeply toxic, for example, cadmium, mercury, and lead. Like in rice was necessarily programming gene under Zn stress, when miR268 in rice was significantly induced. MiR268-Overexpressing in the transgenic plant leaves contained expanded high level of melanin dialdehyde and hydrogen peroxide, and high level of Zn in their seedlings gathered when contrasted with those in wild-type plants. MiR268 goes about as a negative controller of rice''s resilience to Zn stress in revealed outcomes however ecological variances role of miRNAs modulator. With prolonged understanding of the role of miRNAs in stress it has turned out to be exceedingly clear; the miRNA-interceded technique for gene regulation turned out to be increasingly practicable and reliable to increase plant tolerance under Zn stress.As well as agronomic factors during 2018 to check out zinc deficiency and toxicity impact on rice plant by using genetic factor contributing to tolerance field trial was performed in district Vehari. Three replications in Randomized Total Complete Block Design (RCBD) with various zinc dosages and genetic factors on rice crop were spread out. Rice seedling development under zinc not stun by over expression of miR268. The miR268-overexpressing transgenic plant leave contained high amount of melanin dialdehyde , hydrogen peroxide and their Zn level high in seedlings and collected when contrast with individual plant which are in nature of wild-type. These outcomes revealed to under zinc stress micro-RNA268 go about as a negative controller of rice tolerance under stress. At last, micro -RNA assumes a necessary function in plants tolerance to stress. To overwhelming lethality guided the overexpression of gene regulation of gene expression. While the executives framework came about increase development rate of rice and agronomic application while zinc applications was used in appropriate quantity.
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خواجہ حسن نظامی

خواجہ حسن نظامی
افسوس ہے کہ گذشتہ مہینہ ہندوستان کے نامور بزرگ خواجہ حسن نظامی نے ۷۷ سال کی عمر میں انتقال کیا، ان کی جیسی جامع الحیثیات شخصیتیں مدتوں میں پیدا ہوتیں ہیں، وہ ایک خاندانی اور صاحبِ نسبت صوفی، صاحبِ طرز ادیب، ذہین و ماہر نفسیات داعی، کامیاب تاجر، غرض تنہا ایک دنیا اور دلّی کی تہذیب و شرافت کی یادگار تھے، انہوں نے اپنی محنت اور خداداد ذہانت و قابلیت اور سوجھ بوجھ سے نہایت معمولی حالت سے جس قدر ترقی اور شہرت و ناموری حاصل کی، اس کی مثالیں کم ملتی ہیں ان کا طرز انشاء نہایت سادہ مگر دلنشین اور سہل ممننع کی حیثیت رکھتا ہے۔ انہوں نے بہت چھوٹی چھوٹی اور حقیر چیزوں پر جیسے مفید، دلچسپ، سبق آموز اور نتیجہ خیز مضامین لکھے وہ ان ہی کا حصہ ہے، ان کے مضامین کے کئی مجموعے شائع ہوچکے ہیں، ان کی تصانیف کی تعداد سیکٹروں سے متجاوز ہے ، موضوع کا اتناتنوع اور نشیب و فراز مشکل ہی سے اردو کے کسی مصنف کے مضامین اور کتابوں میں مل سکتا ہے، ان کی تصانیف میں غدردہلی کے افسانوں کا سلسلہ شاہکار کی حیثیت رکھتا ہے، انہوں نے درجنوں اخبارات اور رسالے نکالے، ایک زمانہ میں ان کے زیرپرستی نکلنے والے رسالوں کی سارے ہندوستان میں دھوم تھی، ان کے بہت سے شاگرد اور تربیت یافتہ اڈیٹر اور صاحبِ قلم بن گئے،اس لیے اردو زبان کی خدمت کے اعتبار سے وہ اس دور کے اساطینِ اردو میں تھے۔
ان کے ہر کام میں جدت و ذہانت نمایاں تھی، اور ان کی کامیابی کا سب سے بڑا سبب ان کا یہی وصف تھا، ان کے مریدوں اور عقیدت مندوں کا دائرہ نہایت وسیع تھا، جس میں ہندو، مسلمان، سکھ اور امراء و والیانِ ریاست سب داخل تھے، ایک زمانہ میں انہوں نے...

Research on Learning Strategies in Arabic Language Education

The learning of Arabic language like any other foreign language contains four main aspects; reading, writing, speaking and understanding while listening.[i] This learning process can be enhanced if the most appropriate Learning Strategy is used. In this paper the most appropriate Learning Strategy of Arabic Language is suggested. The course outlines for Arabic language are thoroughly studied and several professors and experts of Arabic Language from Pakistan, Egypt, Saudi Arabia and Sudan are interviewed. The author, who himself has vast experience in teaching Arabic language, also had the opportunity to sit in the Arabic language classes to observe various strategies and methodologies adopted by different professors while teaching Arabic. In this paper the time spent on teaching Arabic to the students is also discussed. The appropriate size of the class room i.e, the number of students in Arabic language class also matters in improving the quality of Arabic among the students. The matter of teaching Arabic in Arabic only or in the native language of the students will also be touched in here. As the time has changed and the world is moving ahead on a fast pace, it seems necessary to apply the “Direct Method” while teaching Arabic or any foreign language.[ii] This paper will shed light on what is meant by “Direct Method”. The idea of making the student sit and memorize the dry rules of grammar has become obsolete. The idea of telling the student what part of the phrase is subject or predicate, or what is object and what is a noun or verb, may come later. The idea of memorizing the bulk of new vocabulary in the beginning can also be postponed. Hence a paradigm shift is needed here while talking about the Methodology of Teaching Arabic Language, under the heading of “Direct Method”.   [i]     Muhammad Abdul Khaliq, Professor of Arabic and co-author of 'al-Arabia baina Yadaik'. The author of this research paper had a personal interview with him on 21.03.2014, in the Institute of Arabic Language, King Saud University, Riyadh, Saudi Arabia. [ii]    This method is adopted roughly by some great scholars of Arabic language like Dr. V. Abdur Rahim who taught Arabic language for decades in the Islamic University of Madina, Kingdom of Saudi Arabia. The author was fortunate to meet with him many times and get benefitted from his experience. See for details: Abdurrahim, V. (1999), Arabic Course for English-Speaking Students, Leicester: UK Islamic Academy. See also: Abdullah, F. Ibrahim. (1999), Iqra Arabic Reader. Chicago: Iqra International Educational Foundation. Moreover see: Fawzan, Abdurrahman and others. (2004), Al-Arabia Baina Yadaik, Riyadh: Ministry of Education.

Synthesis and Characterization of Cobalt Ferrite Nanostructures

Coprecipitation method was used to prepare pure and doped CoFe 2 O 4 nanoparticles. Ferric and cobalt salts were used as precursors while oleic acid was used as surfactant. X- ray Diffraction and Transmission Electron Microscopy analysis confirmed single phase of nanoparticles with particle size ~20 nm. Electron transport in CoFe 2 O 4 nanoparticles was investigated using impedance spectroscopy from 300 K to 400 K over wide frequency range (20 Hz - 2 MHz). Impedance spectroscopy of CoFe 2 O 4 nanoparticles revealed a semiconductor to metal transition at ~330 K. The semiconductor to metal transition was attributed to existence of mixed valance states of Fe cations, reverse cation distribution among octahedral and tetrahedral sites and various types of interactions between these cations. Variation of exponent “s” with temperature suggested that overlapping large polaron tunneling was the dominant conduction mechanism in cobalt ferrite nanoparticles. The Mössbauer spectroscopy demonstrated the mixed inverse spinel structure of the CoFe 2 O 4 nanoparticles. X-ray Photoelectron Spectroscopy analysis was carried out to study the oxidation states and environment of Fe and Co cations. Electrical properties of Sn 2+ and La 3+ doped CoFe 2 O 4 nanoparticles were studied in detail. The change in dielectric constant and ac conductivity of CoFe 2 O 4 nanoparticles were observed with dopant concentration. The temperature induced delocalization of charge carriers and metallic phase in Co 0.6 Sn 0.4 Fe 2 O 4 nanoparticles was explained using M(H) loops and impedance spectroscopy. Metallic nature of Co 0.6 Sn 0.4 Fe 2 O 4 nanoparticles above 360 K was attributed to dominancy of delocalized charge carriers Fe 3+ –Fe 2+ /Co 3+ – Co 2+ interactions over localized charge carriers Fe 3+ –O 2− –Fe 3+ /Co 2+ –O 2− –Co 2+ interactions. This was suggested that the wasp - waist magnetic hysteresis loop was due to simultaneous existence of ferromagnetic and antiferromagnetic domains in the system. The M(H) loops of Co 0.6 Sn 0.4 Fe 2 O 4 nanoparticles indicated that at lower temperatures the superexchange interaction was dominant as compared to double exchange interaction while at higher temperatures double exchange interaction becomes more strong. The open M(H) loops of Co 0.6 Sn 0.4 Fe 2 O 4 nanoparticles indicated the absence of magnetic saturation. The temperature dependent electrical behavior of the grain boundaries was reported and discussed in terms of depletion space-charge layer in the vicinity of grain boundaries.