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Chitosan Encapsulatedpolygalacturonase its Kinetic Characterisation for Industrial Expolitation

Thesis Info

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Author

Zuneira Saddique

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=314

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721021512

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Polygalacturonase (PG) is a group of pectinases that possess hydrolytic functions and are capable to cleave the a-1, 4 glycosidic bonds between the two non-esterified galacturonic acid residues in pectin and pectic substances. These enzymes are used commercially, such as paper, textile, cellulose, and food industries and have many important industrial applications. It is the most important tool for the purification of galacturonic acid and degradation of pollutants. In the present study substrates orange peel and sugar cane bagasse were used for the production of galacturonic enzymes by Trichoderma hazrianum through Solid State Fermentation (SSF). The highest yield of enzymes produced was 183.0U/mL. This highest yield was obtained at pH7 temperature 35?C, inoculums size 5mL, moisture level 70%, and days of incubation5 nitrogen level 2g.The purified enzyme has been further selected for ammonium sulphate precipitation and gel filtration chromatography. The purified enzyme has been characterized through enzymatic kinetic for study of its characterization temperature 45?C, pH8 and Km0.25, Vmax0.05 values. The immobilization was observed through chitosan. The immobilized enzyme showed the highest activity at temperature 45?C and pH6. For industrial application its activity was observed to decrease turbidity and increase fruit juice clarification.
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