Search or add a thesis

Advanced Search (Beta)
Home > Genetic Polymorphism of Hsp70 in Red Sindhi Cattle Breed of Pakistan

Genetic Polymorphism of Hsp70 in Red Sindhi Cattle Breed of Pakistan

Thesis Info

Access Option

External Link

Author

Asma Saeed

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=320

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721023276

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Thermal pressure alters the normal body homeostasis and cause rigorous injurious sound effects on production of cattles. Dairy cattles are extra responsive to high temperature as they spawn further energy expanded rate and this matter grasp particular consideration when the entire picture of inclusive warm up is measured around the globe. Heat fright proteins are ever-present, highly conserved proteins that cooperate a significant task in cell endurance through unusual stresses. The present study was undertaken to assess peculiar relevance of HSP70 gene with heat tolerance ability in Red Sindhi (Bos indicus), tropically evolved breed of Pakistan. The aspiration of this study was to explore and portray the heat shock proteins gene diversity in coding region of Red Sindhi cattle to understand their thermotolerance under hot conditions using Polymerase Chain Reaction (PCR). The complete coding region of HSP70 is 1926bp long without any intron, programming a protein of 641 amino acids located on chromosome No.23, with predicted molecular weight (Mw) of 70,190.56 kDa. The functional parts of HSP70 protein consist of an amino-terminal or N-terminal 44kDa ATPase domain (amino acid residues 1-386), 18kDa domain is peptide binding (a.a 384-543) and highly conserved 10kDa Carboxyl or C-terminal domain (residues 542-640) in all eukaryotes. The phylogenetic examination of mammalian HSP70 gene indicated that Red sindhi has showed (99.79 to 99.48%) homology with Bos taurus, Bubalus bubalus and Capra hircus while least similar relationship was observed with Canis lupus that was 86.34%. The results express that nucleotide as well as deduced amino acid order was greatly preserved. Comparative sequence analysis of Red sindhi HSP70 gene revealed a total of 14 single nucleotide polymorphic sites, of these 5 were non-synonymous (C14T, G970T, C1469T, A1535T, G1560T) caused change in amino acid sequence while 9 were synonymous. In non-synonymous SNPs the 1 one was reported and four novel type. These 9 mutations caused no change in amino acid. 8 out of 14 were transition type in which purines were replaced by just purines and pyrimidines replaced by other pyrimidines. 6 types were transversion mutations in which purines replaced by pyrimidines and vice versa. At protein level, Red sindhi HSP70 gene has showed total 5 amino acid changes and two of five are in N-terminal domain (M5T, A324S), remaining are in peptide binding domain (A490V,K512M, Q520H). Probably damaging functional impact of amino acid change is seen in two of expressed SNPs. These transformations can be worn as molecular genetics pointer to aid assortment for anti-heat stress cattles. The findings of this study revealed that detected polymorphisms may be associated with ability of bovine to adapt with tropical and harsh climatic conditions but additional exploitation still required to draw the ending about molecular roots of thermotolerant cattles.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

پروفیسرخواجہ حمیدالدین شاہدؔ

پروفیسر خواجہ حمید الدین شاہد
پروفیسر خواجہ حمید الدین شاہد نے ۲۲؍ اکتوبر ۲۰۰۱؁ء کو ۵ بجے شام کراچی میں وفات پائی، وہ اردو کے مشہور ادیب، محقق، صحافی اور شاعر تھے، پاکستان جانے سے پہلے وہ ’’ادارۂ ادبیات اردو‘‘ حیدرآباد کے تمام کاموں میں پیش پیش اور ادارے کے روح رواں ڈاکٹر سیدمحی الدین قادری کے دست راست رہے، عرصے تک ماہ نامہ ’’سب رس‘‘ کے مدیر بھی رہے۔
مرحوم نے عثمانیہ یونیورسٹی کے مختلف کالجوں میں اردو کے استاد کی حیثیت سے بھی نمایاں خدمات انجام دیں، اردو میں انہوں نے جو تصانیف یادگار چھوڑی ہیں ان میں ’’ارمغان امجد‘‘، ’’مثنوی تصویر جاناں‘‘ مصنفہ لچھمی نرائن شفیق ’’اردو میں سائنسی ادب‘‘، ’’رسالہ محمود خوش وہاں‘‘ اور ’’حیدرآباد کے شاعر‘‘ قابل ذکر ہیں۔ ۱۹۴۰؁ء میں ادارۂ ادبیات اردو کی دس سالہ سرگرمیوں کی مبسوط تاریخ ’’سرگذشت ادارہ‘‘ کے نام سے مرتب کی تھی جو وہاں سے شائع ہوچکی ہے، اس کے علاوہ رسالوں میں ادبی و تحقیقی مضامین بھی لکھتے رہے، شاعر بھی تھے لیکن کوئی مجموعہ شائع نہیں ہوا۔
پاکستان منتقل ہونے کے بعد بھی حیدرآباد اور ادارۂ ادبیات اردو ان کے دل و دماغ پر چھایا رہتا تھا۔ اپنے مکان کا نام ’’ایوان اردو‘‘ رکھا اور کراچی سے ماہنامہ ’’سب رس‘‘ جاری کیا اور اس کا ’’زور نمبر‘‘ کا اور حیدرآباد کی طرح کراچی میں بھی وہ اردو کی خدمت اور فروغ کے لیے سرگرم عمل رہے، بہادر یار جنگ اکیڈمی سے بھی تعلق تھا۔ اﷲتعالیٰ اردو زبان و ادب کے اس مخلص خادم کی مغفرت فرمائے اور ان کے اعزہ و اقربا کو صبر جمیل کی توفیق بخشے۔ (ضیاء الدین اصلاحی، جنوری ۲۰۰۲ء)

أساليب الحافظ الزيلعي في نقد متون السنة من خلال نصب الراية

The methods of Al-Hafiz Al-Zaili in criticizing of text of the Sunnah as described in his book Nasbu Al-Raya in analyzing the hadiths of Hidayah. The science of criticism is well known science since the era of the Companions, and critics of the hadith of the honorable companions have played a very important role in the field of narration and carefully criticizing it. And by passage of the era of the Companions and beginning the era of the followers (tabieen), the criticism became more obvious depending on the growing need, especially after spreading of lie, and creation of fake hadith, which led the critics to further research and verification, for scrutiny between the narrations and then differentiation between the right and the weak. The imams and scholars of hadith from the era of the Companions till present continuously inheriting the approach of criticizing the narratives in succession of their predecessors, whether criticizing the narrators or the texts. I have seen that it is worthwhile to stand on the efforts and methods of one of the imams in his criticism of the hadiths and I have chosen the effort of Imam Hafiz al-Zaili through his book " Nasbu Al-Raya in analyzing the hadiths of Hidayah" to learn how he was using the standards traded among the scholars of Hadith for textual criticism of Sunnah. As the those denied the hadiths from orientalists and their followers and those who follow their example simply claim that the scholars of hadith did not criticize the Sunnah in true criticism and even if they have criticized the hadiths, their criticism was only concerning the narratives not the text, now it is clear through this article that the scholars of hadith did not leave the side of the text, but they criticized text as they criticized the attribution of the hadiths. They set solid rules, which remain scholarly proven and accurate forever. We will revolve in this article around the following topics: learning about Al Hafez Zaili and the science of criticism, methods of textual criticism according to Hafiz Zaili, by focusing on: Criticism of the hadiths for violating the explicit meaning of the Qur'an, or for contradicting the Sunnah, or for contradicting the explicit consensus, or for risking and exaggerating the promise or the warning of simple action, or lack thereof in books of hadith These are the most important rules sited by al-Hafiz al-Zaili, which he practiced and criticized the hadiths and distinguished them between the correct and the weak.

Residual Status and Air-Soil Exchange Fluxes of Persistent Organic Pollutants in the Environment of Punjab Province, Pakistan

Contamination of different environmental compartments through persistent organic pollutants (POPs) is one of the most impending threats globally. The present study aims to investigate the first systematic data on the levels, distribution, possible sources and air-soil exchange fluxes of persistent organic pollutants (POPs) including organochlorine pesticide (OCPs), polychlorinated biphenyl (PCBs), polybrominated diphenyl ether (PBDEs) and dechloran plus (DP) in the air, surface soil and sediment samples from agricultural and industrial areas of Punjab Province, Pakistan. The present study was conducted in the catchment area of River Ravi from Punjab Province which is the most populated province of Pakistan with a population >90 millions; approximately 56 % of the total population of the country. Surface soils and air samples were taken from ten (10) sampling stations in Punjab Province, while seven (7) sampling stations were selected on the River Ravi to collect surface sediments. Air concentrations of POPs were estimated by using the polyurethane foam passive air sampling (PUF-PAS) technique. Air–soil exchange of POPs was estimated by calculating the fugacities in soil and air samples. Concentrations of ΣOCPs and ΣPCBs were ranged from 121-705 pg m-3 and 35-389 pg m-3 for air samples, 24.6-248 ng g-1 and 6.7-45 ng g-1 for soils and 2.7 to 99 ng g-1 and 4.6 to 424 ng g-1 for sediments, respectively. DDTs and HCHs were dominant OCPs in all air, soils and sediments while among PCBs, tri-, tetra- and penta-CBs was frequently detected homologues. Comparison of OCPs and PCBs concentrations with available sediment guidelines indicated severe contamination of DDTs and PCBs in the study area. Different indicative ratios for organochlorine residues in both soils and sediments suggested current use, long range transport along with past application of these chemicals to the total burden. WHO-TEQ values of dioxin like Σ10PCBs for soil samples obtained were very high and met the limitations, recommended by the Canadian Council of Ministers of the Environment (CCME). Levels of ΣPBDEs and DPs ranged from 8.2-124.7 and 1.5-529 pg m-3 for air, 0.6-501 and 0.1-15 ng g-1 for soil and 1.0-2599 and 0.3-4.7 ng g-1 for sediment samples, respectively. BDE-209 was the most abundant PBDE congener, indicating that deca-BDE accounts for most of the total PBDE emitted in the environment of the Punjab Province. The lower average fractions of anti-DP showed significant differences to those of the technical mixtures, indicating lack of DP production source in Pakistan. In general, POPs level in the current study were found lower and/or within the range of other studies reported throughout the world. Conversely, OCPs and PCBs concentrations in riverine sediments were found much higher than previously reported in Pakistan. Air–soil exchange of POPs was estimated by calculating the fugacities in soil and air samples. In the present study, fugacity fractions (ff) values suggested that soils are acting as a secondary source of DDTs to contaminate the atmosphere at certain sampling stations while other areas showed equilibrium and/or atmospheric deposition status. By our results, it is concluded that globally banned organic pollutants are still used/emitted in the catchment area of River Ravi. Our findings also drew attention that elevated levels of DDTs, HCHs, PCBs (tri- and tetra-), and BDE-209 in the study area must be considered as an important environmental issue and steps should be taken to control excessive discharge of organic pollutants in the local environment. The current study also encouraged to conduct more detailed studies to evaluate the ecotoxicological effects of these contaminants in the environment of Pakistan.