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Home > Genetic Diversity and Phlogenetic Relationship of Rohu Fish Labeo Rohita from River Chenab and its Reserviores by Using Simple Sequence Repeat Method

Genetic Diversity and Phlogenetic Relationship of Rohu Fish Labeo Rohita from River Chenab and its Reserviores by Using Simple Sequence Repeat Method

Thesis Info

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Author

Hafiz Mulazim Hussain

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=347

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721029118

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Labeo rohita (Rohu) is naturally occurring fish in the rivers of Pakistan, Bangladesh, India, Terai region, Nepal, Burma, Sri-Lanka, Thailand, Vietnam and Laos etc. Despite its wide distribution and status genetic data of Pakistani population is not available in the literature. Genetic measurement of a species is not only significant for specific administration but also for understanding its genetic variations. Various genetic and molecular methods are available for the complete genetic analysis and phylogenetic relationships of species. This study involves simple sequence repeat markers for the determination of genetic variation between various samples of Labeo rohita specie, which were collected from Head Muhammad Wala, Muzaffargarh and Khangarh regions of river Chenab, Pakistan. DNA was extracted from the muscles of fish by (phenol:chloroform:isoamylalcohol) extraction method. PCR was performed for the amplification of microsatellite regions. Through Polyacrylamide gel electrophoresis (PAGE), scoring of bands for alleles was performed for the generation of genotypic data which was then used for various indications of genetic diversity. Mean value of polymorphism for all markers was 78.63%. Out of 543 loci detected 427 were found polymorphic in all samples. Allele number fluctuated from 2 ? 7 with average 3.58 values and allelic frequency was from 0.3333 to 1.0000 with average 0.6389. Gene diversity found was from 0.0000--0.7901 with average 0.5624 and PIC value was 0.0994 -- 0.7618 with average. 0.4988. Mostly values of Nei?s genetic distance among different populations of different areas of river Chenab were 0.6667, 0.7500, while values of Jaccard?s similarity coefficient were 0.7500 and 0.9167.These values indicate that genetic makeup of L. rohita individuals of different areas of river Chenab differ from each others. This difference may be due to anthropogenic interventions, reproductive isolations and environmental factors. Phylogenetic tree on the base of UPGMA divided all samples into three clusters. C1 xv contains all samples of Khangarh region, C2 contains samples of Head Muhammad Wala and C3 contains all the samples of Muzaffargarh region. Cluster analysis shows that samples of one region show similar genetic information but genetic differences are more with the fish of other regions. It means that geographic isolation and different aquatic conditions have effect on genetics of organisms.
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دور اُن سے دہر کی ساری بلائیں ہو گئیں


دُور اُن سے دہر کی ساری بلائیں ہو گئیں
جن کی قسمت میں مدینے کی فضائیں ہو گئیں

ہم کو پہلے مل گئے رب کی عطا سے مصطفی ؐ
مصطفی ؐ کی ہم پہ پھر ساری عطائیں ہو گئیں

آمدِ محبوبِؐ ربِّ العالمیں کے فیض سے
فیض یابِ نعمتِ رب ساری مائیں ہو گئیں

عرش پر بھی بہرِ امت در گزر کی اِلتجا
فرش پر بھی ’’ربِّ ھب لی‘‘ کی دُعائیں ہو گئیں

سبطِ پیغمبر پہ جاں کچھ اس طرح قربان کی
حضرتِ عباسؓ پر قرباں وفائیں ہو گئیں

فاتحِ مکہ کا وہ اظہارِ امن و آشتی
محوِ حیرت امن کی سب فاختائیں ہو گئیں

آپؐ کا سجدئہ محشر کام آیا اُس گھڑی
پیشِ داور عاصیوں کی جب خطائیں ہو گیئں

گنگ لہجوں کی صدا آقاؐ عطا ہوں مدحتیں
مسکرا کے آپؐ نے فرمایا! ’’جائیں ہو گئیں‘‘

مَزرعِ ہستی پہ بارِش ہو گئی انوار کی
ظلمتیں مٹتی گئیں عرفاںؔ! ضیائیں ہو گئیں

سیرت نبوی ﷺ: ایک تحقیقی جائزہ

Prophet Hood consists of guidance from Allah to humankind. It is a Allah given blessing and a favor that is bestowed on an individual chosen be Him to convey His message, which cannot be acquired or earned otherwise. There has never been a human being so well-respected, loved and followed as Muhammad (SAW), the final messenger of Allah. There has never been a person who has changed world history so dramatically as Muhammad (SAW) and his message. The Prophet (SAW) was the single most important person in the history of the world. Knowledge of the Prophetic Biography is necessary for every Muslim and sharing it with everyone is a responsibility. The importance of a complete biography of the Messenger as available to us cannot be under estimated in this troubled time since both Muslims as well as Non-Muslims have serious knowledge gap when it comes to even approaching the nature of the Final Prophet and the Ultimate Messenger of God sent to all of humanity, who came to restore the primordial religion of Man, the submission to Allah and His Commands. Muhammad (SAW) serves as: - Allah’s messenger and prophet to all mankind as an example of human behavior and noble character Therefore, in studying his life-story we should derive lessons and morals that can help us in our lives today.

Evaluation of Different Bait Formulations for the Management of Indian Crested Porcupine, Hystrix Indica Kerr

Field trials were conducted in Abbotabad-Balakot tract (Pakistan), during April 2004 to January 2007 to select cheap, locally available and suitable bait formulations for Indian crested porcupine (Hystrix indica) and tested these for their potentials to carry lethal quantities of rodenticides. Results of no-choice, multiple-choice and paired-choice tests revealed that groundnut was preferred, both in the whole and cracked forms, over maize, wheat, millet, rice, gram and oats. All the food items were consumed in significantly higher quantities than in their whole form, except for rice. Consumption of all the food grains significantly increased with increasing length of its exposure to the novel food, indicating a careful nature of the species in selecting new food. No-choice tests revealed that groundnut - maize 1:1 mixture can be cost effective bait base and its consumption was not signiifcantly different from that of pure groundnut. Saccharin (5%) supplemented groundnut – maize (1:1) bait was consumed in significantly higher quantities, while all other aditives (common salt, egg yolk, egg shell powder, fish meal, peanut butter, mineral oil, bone meal, coconut oil and yeast powder) at both 2% and 5% concentrations and saccharin at 2% concentration did not significantly increase the intake of groundnut – maize (1:1) bait. Consumption of bait, offered after 3 days of pre-baiting, significantly declined when poisoned with 3% zinc phophide and such effects were delayed with 2% and 1% zinc phosphide. Groundnut – maize (1:1) poisoned with 2% zinc phosphide resulted in maximum decrease (55%) in porcupine burrow activity. Strychnine alkaloid (2%) added groundnut – maize (1:1) bait was seriously shuned by porcupine and caused minimum reduction (25%) in burrow activity. Coumatetralyl (0.0375%) added bait caused the highest reduction (80%) in burrow activity. Saccharin (5%) supplemented poison baits were more acceptable and caused some 10% higher reduction in burrow activity using allpoison cereal bait formulations. Field trials on fresh food items suggested that guava was preferred over potato, carrot and sweet potato. Peanut butter did not significantly increase consumption of guava. Consumption of zinc phosphide impregnated guava, offered after 3 days of pre- baiting, exhibited a decline and no bait was consumed on 3 rd day of poison baiting. Decline was the most rapid with 3% zinc phosphide and minimum with 1% zinc phosphide. Maximum reduction in burrow activity (55%) was recorded with 2% zinc phosphide, followed by 1% (35%) and 3% (25%). Fumigation of porcupine burrows with aluminium phosphide tablets produced 100% reduction in burrow activity, by applying 8 tablets per burrow, 85%, by 6 tablets and 75% by 4 tablets per burrow. A 100% reduction in burrow activity was recorded by applying 4 tablets of aluminum phosphide per burrow in small, 6 tablets in medium and 8 tablets in large burrows. The present study suggested that initial control of the porcupine can be achieved by applying 2% zinc phosphide using guava or 1:1 groundnut – maize mixture supplemented with 5% saccharin. Coumatetralyl (0.0375%) poisoned bait can give a higher control of porcupine, yet involves a higher labour cost and can used as a second line of action. Fumigation of burrows with aluminum phosphide is though cost effective and can be used in the porcupine management programme, yet is non- specific in its action and hence can be pressed into action under emergent conditions only.