Search or add a thesis

Advanced Search (Beta)
Home > Tetra-Primers Based Genotyping of Rs6511720 Polymorphism in Ldlr Gene and Association With Ldlcholesterol Levels

Tetra-Primers Based Genotyping of Rs6511720 Polymorphism in Ldlr Gene and Association With Ldlcholesterol Levels

Thesis Info

Access Option

External Link

Author

Zarina Bashir

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=366

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721034871

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Dyslipidemia is an abnormal amount of lipids (e.g. triglycerides, cholesterol and/or fat phospholipids) in the blood. It is classified as presentation in the body (together with the specific type of lipid that is increased and underlying cause for the ailment (genetic, or secondary to another condition). Low density lipoprotein receptor gene is known as most important in the development of dyslipidemia. In this study, blood from patients (n=25) and normal individuals (n=25) were used to estimate lipid profile and DNA extraction. Lipid profiling was done through spectrophotometer technique. DNA extracted using standard organic method. DNA quantification will be done on agarose gel electrophoresis and nano-drop. Tetra-primers were designed for rs6511720 polymorphism in the LDLR gene to genotype through ARMS PCR technique. Designed ARMS PCR primers will be useful run on agarose gel to identify the genotype. Genotyping data was analyzed using statistical technique. 389bp was achieved in all of the samples which was control band and will be used to produce allele specific bands i.e. of 257bp and 192bp. Though the intensity of control fragments was weak, the samples are correctly genotyped as evident from the sequencing and BLAST results. Statistical analysis has shown the conformance with HW-principle showing that the samples were collected randomly. But association was not found because of low sample size. T-ARMS may be proven as rapid test to diagnose the mutation in LDLR gene causing the early prognosis of the dyslipidemia
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

ورفعنا لک ذکرک

ورفعنا لک ذکرک
نصیر احمد اخترؔ
نعت وہ لفظ ہے جو اردو ادب میںمحبوبِؐ خدا کی توصیف و ثنا کے لیے مختص ہے۔
نعت کے سلسلے میں پروفیسر ریاض احمد قادر ی کے خوب صورت الفاظ ہیں :
’’نعتِ رسولِؐ مقبول درود شریف کی شعری صورت ہے۔‘‘
نعت کے بارے میں ممتاز حسین کی رائے یوں ہے:
’’ہر وہ شے نعت ہے جس کا تاثر ہمیں حضورِ ؐرحمۃ للعالمیں کی ذات گرامی سے قریب لائے‘‘
’’ورفعناکی صدا ‘‘ میں اس عظیم ہستی کے اوصافِ حمیدہ بیان کیے گئے ہیں جن کی توصیف میںخود ربِ کائنات نے فرمایا’’ورفعنا لک ذکرک‘‘۔محبوبؐ خدا کا ذکر،چاہے درود کی صورت میں ہو یا نعت کی ،دراصل ورفعنالک ذکرک کی تفصیل ہے ۔
نعت کسبی نہیں وہبی ہے۔نعت کا شعر اس وقت ہی زبان کو معطر کرتا ہے جب دل حبِ مصطفیؐ سے لبریز ہو۔محمد عرفان علی خوش نصیب ہیں کہ ان کا نام بھی مقصودِ کائنات کے ثناخواں اور ثنا گو کی فہرست میں شامل ہے۔ خوب صورت شخصیت کے مالک ،عشقِ مصطفیؐ سے مالا مال ہیں۔نعت کی سعادت خدا کی خاص عطا ہے۔ اس لطف و کرم کے بارے میں محترم طالب حسین کوثری نے کہا ہے۔
ہر سخنور کو کہاں ہوتی ہے توفیقِ ثنا
ہاں مگر جس پر خصوصی ہو نِگاہِ مصطفیؐ
نعت کے لوازمات میں سے محبوبِؐ خدا سے والہانہ محبت بنیادی حیثیت رکھتی ہے۔جو شاعر جتنارسول ؐ خدا کی محبت سے سرشار ہو گا۔اس کے کلام میں اتنا ہی سرور و کیف زیادہ ہو گا۔جناب محمدعرفانؔ علی کی نعتوں میں جو کیف و سرور نظر آرہا ہے وہ محبت ِمصطفیؐ کا ہی ثمرہے۔ان کے دل میں آپؐ سے والہانہ محبت کا جذبہ پوری آب و تاب سے کار فرما ہے ۔یہ خاص نعمت ایک تو خدا کی دین ہے دوسرا ان کے والدِ محترم محمد حسین صاحب بھی...

سورۃ الحجرات کے تناظر میں اسلامی معاشرے کے خدوخال

In this article, some basic elements of Islamic society have been clarified in the light of Surah Al Hujurāt.  Just as a solid foundation of a building is essential for a strong building, so a strong foundation is essential for a successful society and a strong foundation of an Islamic society has its principles, which are derived from the Qur'an and Hadith. Since these principles are important for the well-being and development of the Muslim society, their observance is necessary for the Muslim society.  This article presents a picture of an ideal society in the light of Surah Al Hujurāt. Have presented the following topics in this article:  Role of leadership and its elements, unity of Islamic society, ethics and beliefs are some of the principles discussed in this article. The research concludes that the principles stated in Surah Al Hujurāt play a vital role in establishing a stable Islamic society.

Intuitionistic Fuzzy Rough Graphs With Applications

Intuitionistic fuzzy sets, neutrosophic sets, rough sets and soft sets are different mathematical tools to deal the problem of how to understand and manipulate imperfect knowledge. The intuitionistic fuzzy rough framework and soft rough neutrosophic framework are made by combining these models, which are more flexible and expressive for modeling and processing incomplete information in information systems. The notions of intuitionistic fuzzy rough sets and soft rough neutrosophic sets are applied to graph theory in this thesis. Novel hybrids models, namely, intuitionistic fuzzy rough graphs and soft rough neutrosophic influence graphs are introduced. Certain methods of their construction are developed. Further, efficient algorithms are developed to solve decision-making problems. The time complexity of the algorithms is computed. Moreover, MATLAB coding of the algorithms is given for the implementation of the proposed hybrid models.