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Home > Genetic Screening and Molecular Characterization of Hbv and Hcv in Rural and Urban Population of District Muzaffarabad Azad Kashmir by Using Nested Pcr

Genetic Screening and Molecular Characterization of Hbv and Hcv in Rural and Urban Population of District Muzaffarabad Azad Kashmir by Using Nested Pcr

Thesis Info

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External Link

Author

Muhammad Naveed

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=369

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721035706

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The infection of liver is known as hepatitis. It is observed that more than 2 billion people got life-threatening sickness due to HBV and more than 230-300 million people suffered from HCV around the globe. The present study dealt with 200 samples that were collected from rural and urban population of Muzaffarabad AJ&K. Both male and female genders of 15-70 years of age were included in the sample collection process. For detection of the presence of virus, initially ICT method was used for 200 samples. Among them only 11 sample from rural and 29 samples from urban population showed positive results of hepatitis virus B and C. Those samples were further confirmed for viral DNA and cDNA by using nested PCR. After performing nested PCR 1 (1%) individual of HBV and 4(4%) individuals of HCV were found PCR positive from rural and urban population. During gel electrophoresis the band size 230bp of HBV and 215bp of HCV were obtained on gel. Furthermore, the phylogenetic analysis was done after gene sequencing of PCR positive HBV and HCV samples. There were 99% resemblance of both HBV and HCV DNA sequence to the sequences present in Genbank by using NCBI Blast. By using MEGA7 program, phylogenetic tree was constructed and average pair wise mean distance obtained of HBV was 0.0037 and 0.23 of HCV. Moreover, the statistical analysis of the study showed risk factors assessment results from different carriers e.g., haircut from barber shops and local clinics. They gave significant values p=<0.0001 and p=<0.0001 respectively for spread of HBV and HCV in the studied population. The final results of study showed that the risk factor of hepatitis virus B and C was higher in urban population rather than in rural population.
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پروفیسر مسعود حسن

پروفیسر مسعود حسن
(پروفیسر مختارالدین احمد)
علمی و ادبی دنیا میں یہ خبر افسوس کے ساتھ سنی گئی کہ ۲۷؍ مئی ۱۹۹۲؁ء کی شب کو عربی و فارسی کے مشہور استاد اور اردو کے ممتاز ادیب پروفیسر مسعود حسن طویل علالت کے بعد کلکتہ میں وفات پاگئے۔ خدا ان کی مغفرت فرمائے اور انہیں جنت الفردوس میں جگہ دے۔
مسعود حسن ۵؍ نومبر ۱۹۲۰؁ء کو اپنے وطن کھگول (دانا پور) ضلع پٹنہ کے ایک ممتاز اہلحدیث خاندان میں پیدا ہوئے جو وہاں کئی پشتوں سے آباد تھا اور اپنے مذہبی اور علمی خدمات کی وجہ سے مشہور تھا۔ ان کے والد منشی غلام قادر نے انہیں ان کے حقیقی ماموں حکیم مولانا محمد حسن (۱۸۸۰؁ء۔ ۱۹۶۱؁ء) کے سپرد کردیا جن کے سایۂ عاطفت میں ان کی ابتدائی تعلیم و تربیت ہوئی، متوسطات کی تعلیم مدرسۂ فیض عام مؤناتھ بھنجن ضلع اعظم گڑھ میں پاکر وہ ۱۹۳۳؁ء میں مدرسۂ اسلامیہ شمس الہدیٰ میں داخل ہوئے جہاں مدرسہ اکزامنیشن بورڈ سے ۳۳؁ء میں انھوں نے ’’مولوی‘‘ اور ۱۹۳۵؁ء میں ’’عالم‘‘ کے امتحانات میں کامیابی حاصل کی وہاں کے اساتذہ میں مولانا محمد سہول عثمانی، اصغر حسین بہاری، مولانا سید دیانت حسین دربھنگوی، مولانا ظفرالدین قادری (۱۸۸۵؁ء۔ ۱۹۶۲؁ء)، مولانا سید شاہ عبیداﷲ (متونی ۱۵ جمادی الاخریٰ ۱۳۵۸؁ھ، مولانا عبدالشکور آہ ؔؔ مظفر پوری اور مولانا سید عبدالسبحان دسنوی کے نام یاد آتے ہیں۔ مولانا محمد سہول، مولانا اصغر حسین اور مولانا عبدالشکور مدرسۂ دیوبند کے تعلیم یافتہ تھے اور شیخ الہند مولانا محمود حسنؒ کے شاگرد، مولانا دیانت حسین نے مدرسۂ عالیہ رام پور میں تعلیم حاصل کی تھی اور وہ معقولات میں مولانا فضل حق خیر آبادی اور مولانا حکیم برکات احمد بہاری ثم ٹونکی کے اہم تلاندہ میں تھے۔ مولانا ظفر الدین قادری، مولانا احمد حسن کانپوریؒ کے شاگرد تھے وہ مدرسۂ منظر اسلام بریلی کے فارغ التحصیل...

اسلامی معیشت اور امداد باہمی کا تصور

Islam is the only religion which is complete code of life. The needs of human beings have been felt and addressed properly. In spite of created by only one creator humans are different in their potentials, behavior and attitude. Owing to this difference, the incomes of people are drastically different from each other. As a result, a few people are rich and well off whereas some are poor- being up to sustenance level. Therefore, people cooperate with each other to eliminate the class differences of social strata. In this perspective, Islam has given an invulnerable perfect socio-economic system. For mutual cooperation, Islam has also addressed the issue and suggested different steps such as madharabah, partnership and tenancy. Bedsides Islam has also focused on secondary resources of mutual cooperation and support that are typically related to the personal concern. It includes: sacrifice, gift, free lending and Qarz-e- Hasanah etc. It also reflects that the man gives precedence to congregational matters over his individuality which is an ample example of mutual cooperation. In fact, mutual cooperation is one of those key factors on which the edifice of prosperous society is built. All religions and ideologies have focused and emphasized on the mutual cooperation; however, Islam lays extra emphasis to build a strong and thriving human state.

Factors Influencing the Formation of Producer Gas from Coal and Biomass in Circulating Fluidized Bed Gasifier

Recent energy crises of Pakistan have triggered the interest of researchers in co-gasification. Little research work is available on Pakistani low-grade high volatile coal and biomasses derived from agricultural waste. Pakistan stands 5th in world coal reserves. Currently, only the sugar sector is fully or partially using the abundantly available sugarcane waste (baggase) as fuel for their combined heat and power (CHP) plants. Rice husk and corncob are also being utilized inefficiently by textile and rice mills to meet their energy requirements. Hence, current low level use of these resources is due to the non availability of an efficient technology. The co-utilization of coal and biomass for gasification in CFB is a new emerging technique. Accordingly, the prime objectives of this research work were to develop and operate the Circulating Fluidized Bed Gasifier (CFBG) and to investigate the influencing parameters that affect the composition of the producer gas. Accordingly, the research work was comprised of two parts; In first part, variety of coal, biomasses and coal-baggase blends (85/15, 91/9 and 94/6) were characterized physically and thermally using a thermogravimetric analyser (TGA-LECO 701). It was operated in non-isothermal mode from ambient room temperature to 950°C to characterized the coal and biomass under various operating conditions such as heating rate (40, 20 &15°C/min), feed composition and equivalence ratio (0.24, 0.30 & 0.35) and determined their impact on thermal conversion. The conversion of biomasses completed at lower temperature (750°C) than to coal and coal-baggase blends that completed at 950°C. Results revealed that biomasses had higher de-volatilization rate than to coal and coal-baggase blends due to presence of higher fraction of volatiles. Even though in sub-stoichiometric environment, increasing heat rate enhanced the conversion, however the optimum conversion was found at heating rate 20°C/min for coal- bagasse blend 91/9 and at equivalence ratio (ER) of 0.30. Further to above, heating values decreased with increasing biomass (bagasse) fraction in the blends. Arrhenius equation model was applied on TGA data to determine the kinetic parameters (activation energy and frequency factors) and observed that these variables also have very pronounced effects the kinetic parameters. The research work mainly focused to develop and operate a Circulating Fluidized Bed Gasifier (CFBG) using the result of characterization studies. Accordingly, a CFBG made of stainless steel (316) with internal diameter 81mm and total height 3900mm fitted with external gas preheater (EGH), stand pipe, cyclone and water cooled condenser was operated for temperature change 700°C to 950°C. The effect of operating parameters; temperature, ER values (0.25-0.38) and coal-bagasse blends (91/9 & 94/6) on the composition of producer gas were investigated. The temperature of the gasifier varied from 750°C to 875°C, a series of heterogeneous and homogeneous reactions took place and the gas composition obtained showed that up to the temperature 750°Cto 820°C the H2/CO molar ratio decreased from 0.82 to 0.77 but after this, H2/CO ratio began to increased and gained the values of 0.81 and 0.87 for temperatures of 850°C and 875°C respectively. Conversely, the molar ratio CO/CO2 increased from 0.57 to 0.67 with temperature variation (750°C-820°C), however a further increase in the temperature (>820°C to 875°C), decreased the CO/CO2 ratio and attained the fraction value 0.65 and 0.62 respectively. Increasing ER value not only enhanced the temperature and superficial velocity of particle but also caused to increase the fraction of CO2 and N2 in the producer gas and decrease in CO and CH4. However, increase in H2 and CO was observed at low ER value. Higher fraction of coal in feed stock, increased the molar fraction of CO2, however, the concentration of CH4 decreased due to decreasing fraction of volatile matters in blended feedstock, as biomasses contained more volatiles than to low rank coal.