This research interest gives a brief concept about toxic effects of heavy metals and its estimation in aquarium fish. In aquatic ecosystem, heavy metals are considered most important pollutants. Majority of the heavy metals tend to accumulate in tissues as well as organs of the body of the fish by consuming polluted water and leads to poisoning of fish. These metals can influence reproduction, growth, weaken immune system and induce pathological effects.In area of Multan city, water may be contaminated with different heavy metals. The use of this water in aquarium may affect the growth of Tiger barb (Puntigrus tetrazona). Effect of heavy metals (Iron and Strontium) on this fish has not determined in area of Multan city. By estimation of Iron and Strontium metals in this fish will provide very useful way and features for scientists doing work in domain of effect of heavy metals on aquarium fish. Fish samples were collected fromarea of Multan city. Each sample of fish (Puntigrus tetrazona) has passed through morphometry then dried and is grind. After grinding, samples were subjected for ash formation. Then ash of each sample was dissolved to form solution. Then elemental analysis is determined by Atomic Absorption Spectrum. Presence of Iron and Strontium was confirmed and its effects were determined on Puntigrus tetrazona.