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Home > Genetic Diversity Analysisof Catla Catla Thaila Fish Collected from Rivers Indus and Chenab Using Microsatellite Dna Marker

Genetic Diversity Analysisof Catla Catla Thaila Fish Collected from Rivers Indus and Chenab Using Microsatellite Dna Marker

Thesis Info

Access Option

External Link

Author

Ghulam Yaseen

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=388

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721039943

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Genetic investigation in fish population is usefull not only to find changes in genetics of species but also to improve management and stock program. Changes in genetics may be either by mutagens or by evolution. Genetic based discrimination among fishes or any other organism by using molecular markers goes back to about 3 decades. Microsatellite markers owing to great variability, supreme divergency and co dominant capacity are widely used in genetic studies. Genetic diversity of Catla catla (Thaila fish) from various sites of two rivers Indus and Chenab (Pakistan) was determined by using 11 single sequence repeat (SSR) markers against 15 samples. Out of 213 loci detected 195 were polymorphic in all samples in this way mean value of polymorphism for all used markers was 91.54 %. Value of effective alleles number (NE ) ranged 1 ? 8 with average of 4.0909, allelic frequency or Shanon Information Index (I) was 0.2667 -1.0000 with mean of 0.5455. Gene diversity or Nei?s genetic diversity (HE ) was 0.0000 -- 0.8356 with mean of 0.5713 while value of PIC (Polymorphism Information Content) ranged 0.0000 --0.8152 with mean of 0.5261. Maximum frequency based genetic distance was 0.9091, 0.8182 between various locality fishes of Chenab and Indus repectively. Similarly Jaccard?s similarity coefficient was 0.8182 and 0.7273 between various zone samples of Chenab and Indus respectively, and finaly phylogenetic tree analysis reflects genetic variations within and between populations of Catla catla of various zones of various rivers. This study indicates moderate genetic variations within and between Catla catla populations of various rivers and various zones of these two rivers. This genetic information is beneficient for proper administration of Catla population to maintain familial make up.
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Preface

Almighty Allah revealed the Holy Qur’an for guidance of the mankind. This guidance cannot be acquired without pondering over the meanings of this book. Different scholars dedicated their lives for understanding and then spreading the message of the Almighty Allah. This book is a study of the work on translations and tafasīr of Holy Qur’an. In Sub-Continent, a large number of religious scholars dedicated themselves for the service of Holy Qur’an.  Their vision was broad and they elaborated meanings of the Holy Qur’an according to their own mental approach They were appreciated and criticized by scholars of different sects of the society. There is a dire need to understand and spread the teachings of the Holy book. Hopefully this work will encourage the readers to be aware of the research of theologians. Shāh Walī Allāh is the most important religious figure of Sub-Continent. He and his sons played very important role in spreading the message of Qur’an. Brief services of this family are elaborated in this book. Scholars of Sindh have contributed in writing translations and commentaries of Holy Qur’an. Some of their contributions are highlighted. The reader will also find introduction of well-known translations and tafasīr of the 20th century. But this introduction is restricted to the Sub-continent. Through this book. the writer wants only to motivate people for learning the Holy Book. It is not claimed that services of all the Qur’anic theologians of Sub-continent are highlighted. This is a matter of pride for the author that things are discussed without any prejudice. The readers are the best judges. Suggestions regarding the improvement will be welcomed. I am thankful to Prof. Dr. Shams-ul-Basar and Dr. Zohaib Ahmad for guidance and Co-operation regarding the publication of this work. May Allah Almighty shower blessings on them. If...

جرم و سزائے ارتداد پر قرآن حکیم سے متعلقہ شبہات کا تحقیقی جائزہ جرم و سزائے ارتداد پر قرآن حکیم سے متعلقہ شبہات

According to Islamic Criminal Law, Apostasy is a crime, severely punishable in this world and hereafter. Some people are of the view that worldly punishment is not mentioned in Quran. Rather the rule has been given that a man is free to choose or give up any religion; no one would be compelled in this regard. This is essential to contemplate this critical matter; What is the accurate interpretation of the verses of the Quran in this regard? How the worldly punishment of Apostasy is derived from Quran? What is the meaning of "There is no coercion in Islam"? Either Islam prohibits every form of coercion or it has also been used in some commands of Islam?  In this article, a critical analysis of these doubtful questions regarding the punishment of Apostasy is presented in the context of the Holy Quran.

Exploring the Role of Psyllium Husk As Therapeutic Agent in Human Subjects

Present research was carried out to evaluate the efficacy of psyllium husk as a therapeutic agent in normal and hypercholesterolemic subjects. For the purpose, dietetic cookies were prepared by replacing straight grade flour with psyllium husk in different combinations @ 5, 10, 15, 20 and 25%. Psyllium husk was analysed for chemical composition, dietary fiber through enzymatic method and functional component arabinoxylan (AX) using GC-MS. Characterization results indicated psyllium husk as a rich source of dietary fiber and arabinoxylan. The treatments of prepared cookies were analysed for physical, chemical and sensory attributes with special reference to dietary fiber, arabinoxylan, gross energy, hardness and color tonality. Physical characteristics showed decline in diameter and spread factor whilst, increase in thickness of treated cookies. Chemical assay revealed higher protein in control cookies whereas moisture, ash, crude fiber & NFE along with dietary fiber and arabinoxylan contents were higher in psyllium based cookies. Softer cookies with low gross energy and better color tone were formed using psyllium husk. Sensory panel explicated cookies quite acceptable however decrease in sensory score was recorded during storage. Considering the results of characterization, dietary fiber and sensory response, one best treatment namely T4 (20% replacement of psyllium husk) was selected along with control for two months efficacy study in human subjects during two consecutive years i.e. 2009-10 (Trial-I) & 2010-11 (Trial-II) for validity of the results. Bio-evaluation trial was carried out in normal subjects following same module in hypercholesterolemics by providing five cookies twice a day to each subject. Results indicated non-significant differences in serum lipid profile and glycemic indices (glucose and HbA1c) of normal subjects after consuming dietetic cookies (T4). Nevertheless, psyllium husk based cookies had positive impact to attenuate the lipid profile of hypercholesterolemic subjects. In this context, elevated base line values of total cholesterol 231.50±6.46, LDL 158.78±8.91, VLDL 35.92±1.36, LDL/HDL 4.99±0.25 and glucose 103.10±7.90mg/dL, were significantly reduced to 202.90±7.26, 134.60±8.13, 30.40±1.05, 4.09±0.17 and 92.07±4.11mg/dL, respectively in the Trial-I. Likewise, significant declining trend was observed in respective traits due to treatment T4 in Trial-II. The percent reduction in total cholesterol was 12.35 & 11.26%, LDL 15.22 & 14.10%, VLDL 13.48 & 12.08% and glucose 10.70 & 12.19%, respectively in Trial-I &II for hypercholestrolemic subjects due to dietetic cookies (T4). However, HDL concentration increased non-momentously during the study period. Likewise, triglycerides level was also affected non-significantly however, a slight decline was recorded. Safety evaluation of dietetic cookies was also carried out simultaneously in both categories of subjects and no sign of abnormality was observed in liver (ALT & AST) and kidney functions (urea & creatinine). It has been observed that high base line value for urea was non-significantly declined after administration of test diet in hypercholesterolemic subjects. Furthermore results for hematological tests depicted safe range in allied parameters i.e. platelets count, ESR, red blood cells and white blood cells indices. It is interesting to mention that the ESR of hypercholestrolemic subjects was momentously affected by using therapeutic diet (T4) as it was reduced to 5.61 and 6.98% in Trial-I & II, respectively. It has been observed that psyllium husk is friendly to the normal subjects nonetheless supportive for the hypercholesterolemic subjects. From the current explorations it is concluded that psyllium husk based cookies (supplemented with 20% psyllium husk) have potential to be used as a functional ingredient against the menace of hypercholestolemia and allied discrepancies in vulnerable segments of the population.