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Characterization of Inhibin Gene in Patientsof Polycysticovarysydrome

Thesis Info

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Author

Farkhanda Haroon

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=390

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721040414

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Polycystic ovary syndrome (PCOS) is multisymptomatic gynecological disorder with high prevalence (5-10%) among human females. Primarily it affects female reproductive system resulting in infrequent menstrual cycles. Visceral adiposity, insulin resistance, hirsutism and infertility are other consequences of PCOS. Genetic as well as environmental factors contribute for the progression of PCOS. Multiple studies revealed association of PCOS with mutations in different genes specifically expressing androgens and androgen receptors. PCOS is an autosomal and X-linked disease. 60-70% of PCOS patients suffer from hyperandrogenism i.e. high level of follicle stimulating hormone (FSH) & luteinizing hormone (LH). While LH/FSH ratio elevates in PCOS patients. Inhibin A downregulates the follicle stimulating hormone (FSH) to promote ovulation and regulate normal menstrual cycle. Inhibin A is a hetero dimer of INHA and INHB subunits. INHA along with Antimullerian hormone can be used as a diagnostic marker for PCOS. This study has been conducted using data of 50 participants. They were classified into two groups, control and experimental group. Out of total 50 participants, 30 were PCOS patients and 20 were healthy control subjects. Blood samples of PCOS patients were collected from Pakistan Institute of Medical Sciences. Extracted DNA from blood was used for amplification of exon 2 of INHA subunit of Inhibin A and B gene. Restriction digestion of Amplified gene segment was carried out with restriction endonuclease. Restriction fragment length polymorphism (RFLP) results showed 30% of PCOS patients having heterozygous mutation (G769A/ rs12720062). Results revealed positive risk of developing PCOS when having A allele at position 769bp in heterozygous state. So, we concluded that there is an association between heterozygosity at rs12720062 and risk of development of PCOS.
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ماڑی والیا

ماڑی والیا رحمۃ اللہ علیہ

ماڑی والیا موڑ مہار وے
موڑ مہار، آ مل ہک وار وے
اُڈ وے کاواں کول سجن دے جاویں
میڈا جا کے رو رو حال سناویں
دکھاں کیتا بہت آزار وے
ماڑی والیا موڑ مہار وے
درد دکھاں دی ماری ہاں میں
کردی نت تیاری ہاں میں
سد کول مینوں ہک وار وے
ماڑی والیا موڑ مہار واے
رکھیاں تاہنگاں یار بلاسی
کدی تے میں تتڑی دے بھاگ جگاسی
نت روندی ہاں زار و نزار وے
ماڑی والیا موڑ مہار وے
اُڑ وے کاواں جا بیٹھ سجن دی بیری
دسیں جا کے کل حقیقت میری

بہت اداس تساڈی بیمار وے
ماڑی والیا موڑ مہار وے
درد دُکھاں نے گھیرا پایا
باہجھ سجن کسے دُکھ نہ ونڈایا
احسان تیرا لکھ ہزار وے
ماڑی والیا موڑ مہار وے
ماڑی دے وچ یار نے جھوکاں لایاں
آ مل ماہی اکھیاں ہن ترہایاں
ایہہ ہویاں بہت لاچار وے
ماڑی والیا موڑ مہار وے
جاندیا راہیا میرا پیغام لے جاویں
ڈھول میرے نوں بہت سلام ولاویں
دل ہویا بہوں بے قرار وے
ماڑی والیا موڑ مہار وے
رب کرے جئے میں ماڑی جاواں
ویکھ سجن نوں دل دیاں سکاں لاواں
تیر ہویا کلیجوں پار وے
ماڑی والیا موڑ مہار وے

ماڑی دے وچ بنیاں بہاراں
ہر پاسے دسدیاں ہُن گلزاراں
کدی میں غریب نوں سد مار وے
ماڑی والیا موڑ مہار وے
قادری خواجہ اُتوں جندڑی وارے
نالے رو رو عرض گزارے
دیہو بخش خطا ہاں اوگنہگار وے
ماڑی والیا موڑ مہار وے

Perceptions of Sindhi Muslims Towards Exogamous Marriages: An Exploratory Study

It has been said that exogamous marriages tend to lead to language shift (Igboanus & Wolf, 2009 & Dumanig, David & Shanmuganathan, 2013). In an early study Gal (1978) found that Hungarian women marrying German men in the city of Oberwat had shifted to German to negate their peasant Hungarian ancestry and to move up the socio-economic ladder. Similarly, David and Dealwis (2011) found in their study of Malaysian Sindhi Hindus that exogamous marriages tend to lead to language shift but is not the only reason for the shift away from the heritage language. Therefore, before mixed marriages take place one should study perceptions of the speech community towards such marriages. In this exploratory study, the perceptions of Sindhi Muslims-men and women and from different socio-economic groups towards exogamous marriages are studied. With this objective, semi-structured interviews were conducted with ten Sindhi Muslim participants (four male adults and six female adults) who live in Karachi. The interviews were recorded, transcribed, coded and themes were generated after formal consent was obtained. The findings show that language choice in the home domain is shifting because of intercultural marriages. Also, such marriages are frequent among Sindhi community members with a high socio-economic status who live in cities. Gender plays an equal role too, as the women respondents showed mixed responses towards exogamous marriages while the male respondents had a more positive perception. Such views and perceptions may affect choice of marital partners and language choice in the home domain.

Effect of Pre-Weaning Diets and Varying Levels of Concentrate During Post-Weaning Period on the Performnace of Female Nili-Ravi Buffalo Calves Upto Age of One Year

Nili-Ravi buffalo is a well-known buffalo breed in subcontinent Indo-Pakistan region and famous for its high milk production ability. Currently, buffalo calves and growing heifers are fed on deprived quality and quantity roughages with poor nutritive values resulting in reduced growth rate, reproduction with delayed attainment of puberty and high mortality. These constraints can be overcome through nutritional management of buffaloes. There is a need for the development of standards for adequate, cost effective provision of colostrum, whole milk/milk replacer and calf starter ration to neonatal calves up to weaning, establishment of nutrient requirements for growing buffalo heifer with aim of more average daily gain to reduce age at puberty and nutrients requirements for lactating buffalo according to their status and stage of milk production. The current study comprises of two experiments and was conducted at Livestock Experiment Station, Bhunikey, Pattoki, District Kasur, Punjab, Pakistan. The first experiment was performed with an aim to check the growth performance of female buffalo calves on whole milk & milk replacer and find out the cost effective and growth rate friendly alternate source of liquid diet. The duration of this experiment was 120 days. Thirty six female calves were selected and randomly divided into three (n=12) different treatments A (whole milk), B (50% whole milk & 50% milk replacer) and C (milk replacer). All the calves were given colostrum for first three days, then whole milk up to 15 days of age and transferred into three treatments. In addition to this all the calves were provided calf starter and fresh water ad-libitum. The calves were given liquid diet @ 10% of their body weight for first two months and then gradually decline of 1% on weekly basis for the subsequent two months. Green fodder was started on three month of age. The average daily total dry matter intake was remained same for all the three treatments but the average daily gain was higher in treatment A (457.38±110.13a) compare to treatment C (362.22±107.83b) but it was same for treatment A&B and B&C, respectively. The mean FCR value was also better for treatment A (3.49±0.56b) compare to treatment C (4.30±1.24a) and it was same for treatment A&B and treatment B&C, respectively. The mean cost/kg gain was higher in treatment A (422.72±70.66a) compare to treatment C (352.97±97.49b) and it was same for treatment A&B and B&C, respectively. Animals had performed well on mix liquid (50 % whole milk: 50% milk replacer) diet and it was more cost effective than other two treatments. The aim in second experiment was to set the standard and cost effective level of concentrate ration for growing female buffalo heifer calves. For second experiment thirty (30) calves were selected from first experiment and were randomly dived into three treatments A, B and C. Treatment A was fed on concentrate ration according to 0.5 % of their body weight, treatment B 1.0 % and treatment C 1.5 % of their body weight. In addition to this all the calves were given ad-libitum green fodder and fresh clean water. All the calves were fed on similar concentrate ration having CP: 17 % and ME: 2.6 Mcal/kg. The duration of this experiment was 8 months. There was significant difference (P<0.05) in mean dry matter intake, protein intake, energy intake and protein per kg gain across all the three treatments and were higher (P<0.05) for treatment C then treatment B and lower (P<0.05) in treatment A, respectively. The average daily gain was remained same (P>0.05) for all the three treatments (497.32±17.92, 503.63±19.09 and 532.77±20.67). The higher feed efficiency was observed in treatment A (0.135±.004a) while it was same for treatment B & C (0.113±.003b & 0.108±.004b), respectively. The average body condition & score, body mass index and blood constituents (RBCs, WBCs, heamoglobin, packed cell volume, mean corpuscular volume, platelets count, lymphocytes, monocytes and granulocytes) were unaffected (P>0.05) by different concentrate levels. Concentrate levels had significantly affected some of serum components (total protein and urea) but some components (glucose & cholesterol) were unaffected by dietary treatments. The values of mean serum total protein and serum urea were found lower in treatment A (6.12±0.17b & 42.34±1.59b) compare to treatment B (6.65±0.23a & 50.08±2.05a) and C (6.79±0.23a & 51.41±2.29a), respectively. The higher values of serum total protein and cholesterol in treatment B & C may be attributed to higher concentrate level in these two treatments. Concentrate levels had significantly (P<0.05) affected some of the digestibility parameters (DM %, CP% and NDF%) while other parameters (organic matter, fat, ash, ADF and urine pH) were remained same (P>0.05) on varying concentrate level diet. The mean body measurements (height at wither, body length and heart girth) were also not affected (P>0.05) by dietary treatments. There was significant difference across all the three treatments in total average daily dry matter intake cost and cost per kg gain. These were lower in treatment A compared to other two treatments B & C. It was observed that mean dry matter, protein and energy intake was lower in treatment A (0.5% of body weight) and weight gain was remained same on all the three dietary treatments. The mean feed efficiency was greater and mean cost per/kg gain was lower in treatment A. So, treatment A was remained more cost effective than other two treatments. Both experiments were planned by keeping in mind the problems of buffalo farmer. Rearing of calves with improved growth rate on least cost feeding regime is important in dairy farming. Milk replacer is an alternate source of whole milk. Most of the buffalo farmers don’t use milk replacer for rearing of calves because of slower growth rate. Mixing of milk replacer with whole milk in 50:50 ratio make the consistency of liquid diet near to whole milk. Feeding of whole milk with milk replacer along with calf starter reduces the cost without affecting growth rate. At this stage farmers should keep in mid the cleaning of feeding pans to avoid the risk of diarrhea. In post weaning period calves’ rumen is fully develop and is completely shifted to solid diet. During this transition phase farmers don’t follow the nutritional requirements of calves, which slow down the growth rate and ultimately increase the age at puberty. As buffalo are efficient converter of low quality diet. If farmers offer concentrate ratio (16-18% CP) to buffalo heifers at the rate of 0.5% of body weight along with ad-libitum green fodder, growth rate can be improved cost effectively.