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Home > Molecular Diagnosis and Phylogeny of Infectious Bursal Disease Virus Ibdv in Chicken During Field Outbreaks in District Chakwal, Pakistan

Molecular Diagnosis and Phylogeny of Infectious Bursal Disease Virus Ibdv in Chicken During Field Outbreaks in District Chakwal, Pakistan

Thesis Info

Access Option

External Link

Author

Umm I Habiba

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=391

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721040595

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Poultry diseases are a matter of serious concern and responsible for extremely large profit making crisis in poultry industry per anum. Outbreaks of infectious bursal disease (IBD) are continuously increasing despite vaccination in commercial broilers. The strains of IBDV currently circulating in Chakwal district broiler flocks are not known. Clinicopathologic analysis and reverse transcriptase PCR were used in 18 poultry farms which were situated in district Chakwal to confirm field outbreaks of IBD. The genetic analysis of the hypervariable part of the?VP2?gene was utilized to describe different features of total 6 isolates of these outbreaks. Arrangement of nucleotides, infer amino acid sequences through phylogenetic analysis of VP2 gene containing hypervariable part were used for dividing IBDV strains into two groups. According to phylogenetic analysis, 5 IBDV strains showed special signatures of amino acid in the VP2 gene as A222, I242, I256, I294, S299 and classified as vvIBDV.They showed 97%?99% identity at the nucleotide level. Furthermore, the sequencing analysis of detected field strains revealed the high similarity and close clustering with vvIBDV strains isolated from India, Pakistan, and China, suggesting geographic and temporal relationships among these strains. Interestingly, one IBDV strain clustered togather with vaccinal IBDV strains and representing 99% sequence likeness with vaccine strains which were dissipated, suggesting possible role of attenuated vaccines in the outbreaks of IBD. Our study revealed circulation of vvIBDV strains in Chakwal broiler flocks and these evidences emphasize the need of further detailed and more systemic approaches to evaluate IBDV diffusion and characterization to design effective control strategies.
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