Chlorpyrifos (ChF) is widely used in agricultural fields and indoor for controlling pests. It sinks in the aquatic sediments and pose toxicity for aquatic organisms including fish. Therefore, the current study was designed to investigate the oxidative stress enzymes, and histological alterations in the liver, spleen, and heart of tilapia (Oreochromis niloticus) due to ChF exposure. Median lethal concentration (LC50 24 H) was calculated as 52.78 ?g/l by exposing fish with different acute concentrations (0, 1, 15, 35, 40, 45, 50, 55, 100 and 150 ?g/l) of ChF. For assessment of sublethal toxicity of ChF, the fish were divided into four groups, one group served as control (C) while three groups (ChF1, ChF2, ChF3,) were exposed to three sublethal doses of ChF. ChF1 group was treated with 3.51 ?g/l (1/15th of LC50) of ChF whereas, ChF2 and ChF3 groups were treated with 5.27 ?g/l (1/10th of LC50) and 10.55 ?g/l (1/5th of LC50) of ChF respectively for 14 days and oxidative stress enzymes, biochemical parameters, behavioral changes and histological alterations were evaluated. It was found that level of Glutathione-S-Transferase (GST), Catalase (CAT), and Superoxide dismutase (SOD) increased significantly (p=0.05) in the gills and liver of tilapia treated with sublethal concentrations of ChF. Study of morphological features exposed that fish in the control groupshowed significantly increase in weight and length (p=0.05) as compared to ChF treated fish. The behavioral changes as reduced feeding rate, eratic swimming, increased surface gulping, aggregation in the corner of tank, hemorrhage, and darkening of skin coloration was observed in all ChF treated groups. The significant elevation in level of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was observed in all groups treated with different sublethal concentrations of ChF. Histological study of liver tissues shown increased number of kupffer cells, hydropic degeneration, dilated sinusoids, congestion, necrosis, and hemorrhage in liver of fish from all treatment groups. In the spleen of fish exposed to ChF increased number of melanomacrophage centres (MMCs), necrosis, congestion, and hemorrhage was detected. Disorganized muscle fibers, cardiac muscle fiber degeneration, coagulative necrosis, congestion in blood vessel and necrosis was perceived in the heart of ChF treated fish. This study concluded that sublethal concentrations of ChF can induce oxidative stress and histological alterations in the tissues of tilapia.
پروفیسر جگن ناتھ آزاد ایک شریف اور باکمال انسان ۲۴؍ اگست ۲۰۰۴ء کو ملک کے مایہ ناز شاعر و ادیب، مشہور محقق و نقاد، اقبالیات کے ماہر اور اس کے پایہ شناس جناب جگن ناتھ آزاد اردو دنیا کو سوگوار اور مغموم چھوڑ کر چلے گئے، وہ اردو، اردو ثقافت، ملک کی گنگا جمنی تہذیب، فرقہ وارانہ ہم آہنگی، رواداری، وسیع المشربی اور بھائی چارگی کے علم بردار تھے، ان کی وفات پر کن کن چیزوں کا نوحہ و ماتم کیا جائے۔ جگن ناتھ آزاد صاحب ۱۵؍ دسمبر ۱۹۱۸ء کو مغربی پنجاب (پاکستان) کے شہر عیسیٰ خیل میں پیدا ہوئے جو ضلع میان والی کی ایک تحصیل تھا، ان کے والد پنڈت تلوک چند محروم پیشے کے اعتبار سے معلم تھے مگر خاندانی شرافت اور کمال کے ساتھ ایک صاحب ذوق اور بڑے قادر الکلام شاعر تھے، ان کا شمار اردو کے استاد شعرا میں ہوتا تھا، آزاد صاحب کو علم و ادب کا فطری ذوق تھا جس کو ان کے والد کی صحبت و تربیت نے بہت چمکا دیا تھا، ان کی ابتدائی تعلیم عیسیٰ خیل میں ہوئی، میانوالی سے میٹرک کا امتحان پاس کیا، ۱۹۳۵ء میں ڈی اے وی کالج راولپنڈی سے انٹرمیڈیٹ کیا اور ۱۹۳۷ء میں گارڈن کالج راولپنڈی سے بی اے کیا، ۱۹۴۴ء میں پنجاب یونیورسٹی لاہور سے فارسی میں ایم، اے کیا۔ وہ شروع ہی سے صحافت سے وابستہ رہے جب وہ انٹر کررہے تھے تو کالج میگزین کی ادارت ان کو سپرد کی گئی، اسی زمانے سے مضامین بھی لکھنے لگے تھے، نظم گوئی کی ابتدا اس سے بھی پہلے ہوچکی تھی، ان کا پہلا مقالہ ’’اقبال کی منظر نگاری‘‘ کے عنوان سے میاں بشیر احمد کے مشہور ادبی ماہنامہ ’’ہمایوں‘‘ لاہور میں چھپا، ۱۹۴۱ء میں ماہنامہ ’’ادبی دنیا‘‘ لاہور کی مجلس ادارت میں شامل ہوئے، ۱۹۴۶ء میں اردو روزنامہ...
The idea of less punishment is accepted internationally and very rapid implementation is observed as in various countries the strong punishment like death sentence is avoided. Likewise a slogan of "Love rather than punishment "is implemented within Education Department of Pakistan. The history of last few years proved that the said slogan affected our moral training and education process very badly. As there is no fear of punishment naturally a person diverts towards easiness and ultimately goes towards laziness. To save the nation from this alarming situation, it becomes necessary to obey the teachings of Holy prophet (PBUH) about the training and educating the human being. In these teachings the combination of love and fear is the basic element of the social training. Both of the methods have importance, love creates interest and fear of punishment develops the habit of hard working. This Islamic principle is a universal truth because the success is the result of interest and hardworking of the individuals. Therefore the slogan of "Love rather than punishment must be avoided, as it has negative effects upon the personality. The golden teachings of Holy Prophet (PBUH) which have the positive effects upon the personality and are proved to be the need of the day.
The nano silica spheres are synthesized through sol-gel process. These spheres have very surface area of around 920 m2/g and pore size of about 4.7 nm diameter and are good support material for the preparation of selective sorbent. The silica surface was activated by reacting with an organosilane coupling agent like 3-aminopropyltrimethoxy silane (APTMS) by dispersion in appropriate reaction medium. This process sufficiently activated the inert silica surface for further attachment of organic moitities. Chelating ligands such as 1-napthyl-2-thiourea (ANTU), thiosemicarbazide (TSC) and capryl pyrazolone (CP) were chemically reacted with activated silica surface and functionalized sorbents are synthesized. FTIR, EDX and TGA analysis confirmed the successful immobilization of the organic moieties to the silica surface. After synthesis and characterization, the performance of the materials were tested as sorbents for the separation of heavy metals like Hg(II), Cd(II), Zn(II), Ag(II), and Cr(III) from their aqueous solutions in batch experimental procedures using radiotracer technique. The results showed that the attached functional groups enhanced selectivity of the sorbent towards target metal ions. These functionalized sorbents displayed fast adsorption kinetics and entrapped high percentage of toxic heavy metals from spiked water samples. The adsorption processes were systematically monitored as a function of pH of the medium, agitation time, metal ions concentration and functionalized silica dosage. Effect of various interfering ions on the adsorption of these metals was investigated. Response of the sorbent and sorption process to temperature is also studied and various useful thermodynamic parameters like change in enthalpy (ΔH), entropy (ΔS) and free energy (ΔG) are derived and discussed. An attempt was made in this study to suggest possible mechanism for the uptake of the metal ion and to validate the experimental data by fitting into sorption models such as Langmuir, Freundlich, and Dubinin Radushkevich D-R. Morris Webber, Lagergren and Richenberg‘s equations were applied to follow the kinetics of sorption process. Practical utility and environmental significance of the work was demonstrated. Regeneration of sorbent was carried out to explore the possibility of reuse of these sorbents. These sorbents could be effectively employed for environmental remediation of toxic heavy metals and will greatly improve the conditions of drinking water. Key words: silica nano particles, covalent grafting, adsorption, pollution abatement, radio tracer, Metals preconcentration, heavy metals, organic-inorganic hybrid