بہاء اللہ کی تعلیمات میں علمی سرقات کا تنقیدی جائزہ

The main objective of human life is to get closeness to Allah Almighty and to worship Him.  For this purpose thousands of prophets were sent to guide the people on the right path. Other prophets were sent for a specific nation, limited time and area. At the end prophet Muhammad (SAW) was sent by Allah as the last and universal prophet. On the other hand it is also a bitter fact that in all the times and area there are some people who do not follow, become hurdles and even misguide the others (for their cheap worldly benefits). ╓ussain Alī Mazandrānī is one of those false and fake people. He wrote a book, namely, Kitāb-e-Aqdas and claims it to be the word of Allah, revealed on him, while the fact is that Qur’ānic commands (orders) have been presented in his book according to the will and convenience of people. In some places the very text of the Holy Qur’ān has been copied. The given article discovers this plagiarism and critically analysis the commands written in Kitāb-e-Aqdas in the light of the Holy Qur’ān. And this has been proved with arguments that the Holy Qur’ān is the word of Allah, which is completely safe, while Kitāb-e-Aqdas is the outcome of human efforts written by ╓ussain Alī. Hence it is unsafe and not free from mistakes.

Development of Microbial Proteases for Industrial Applications

Enzymes are the biological macromolecules that speed up the conversion of substrates into products by lower the activation energy of the reacting species. Protein hydrolyzing enzymes (proteases) consist of the major assembly of enzymes being used in industry with a diversity of applications in the food, detergents, leather and meat industries. Due to increasing industrial demands, interest has been growing in microbial proteases for application in different commercial scale processes. Thus, there is need to explore new sources of alkaline proteases. The aim of this research was to identify potential protease producers. Out of six microbial strains Bacillus licheniformis and Aspergillus flavus were found to be the best producers of protease. To enhance the production of proteases by selected strains, different physical parameters, e.g., pH, temperature, inoculum size and fermentation time, were optimized by applying response surface methodology (RSM) under central composite design (CCD). Similary, nutritional parameters such as carbon and nitrogen source were also optimized and the influence of C:N ratio and metal ions on the yield of proteases, were also determined. The proteases obtained were purified by ammonium sulphate precipitation followed by dialysis, ion exchange and gel filtration chromatography methods. The purified proteases were immobilized by synthesizing cross-linked enzyme aggregates (CLEAs) and by optimizing the different parameters for CLEAs formation by RSM under CCD. SEM (scanning electron microscopy) of CLEAs were performed for the presence of protease in CLEAs. Free protease and CLEAs were compared by determining the effect of substrate (casein) concentration, pH and temperature. Free and CLEAs of proteases were used for industrial applications. Concerning industrial applications, alkaline proteases efficiently remove blood stain from fabric, remove the gelatin layer from used X-ray film, degrade animal bones protein, clot milk and dehair animal hides.