فرنگی تیرے ہر دربار سے انکار کرتا ہوں
سنو میں منتِ اغیار سے انکار کرتا ہوں
مری نسلوں کی آزادی جو گروی رکھ کے آ جائے
میں اس ننگِ وطن سردار سے انکار کرتا ہوں
مری ہر ایک جنبش پر ہے میری سوچ کا پہرہ
فسادی قوم کے افکار سے انکار کرتا ہوں
مرے عزم و یقیں کی ترجمانی جو نہیں کرتی
میں ایسی کھوکھلی للکار سے انکار کرتا ہوں
مرے بازو کی طاقت ہی کنارے پر لگائے گی
بھنور میں ہوں مگر پتوار سے انکار کرتا ہوں
مری مٹی بھی سونا ہے مجھے کیسے خریدو گے
تمھارے درہم و دینار سے انکار کرتا ہوں
جو اپنے جھوٹے وعدوں سے محل تعمیر کرتا ہے
میں ایسے کھوٹے منصب دار سے انکار کرتا ہوں
مری غیرت کے بدلے میں جو میرے نام ہو جائے
میں ایسی خلعت و دستار سے انکار کرتا ہوں
مرے الفاظ کی صابر دلوں پر حکمرانی ہے
قلم پکڑا ہے میں تلوار سے انکار کرتا ہوں
Islam is the religion of peace as is evident from the very meaning of the word Islam which is derived from root word \" salam\" meaning peace and obedience. The advent of Islam has been a pannaca for all humanity and, ever since its inception, it has the distinction of contributing more and more to the betterment and progress of humanity. Its statute is crystal clear over all religions and systems. But unfortunately, there has been a systematic campaign to present Islam as a religion of violence. The orientalists who tend to misinterpret Islam by relying on some injunctions without looking into their specific context. The international media, under the influence of Jews, is also spreading a false propaganda. Moreover, certain elements with in Islamic world are also providing ample opportunity for the same. In such circumstances there is a dire need to present the true spirit of Islam but such attempts are lacking in the Muslim world despite that it is one of the major concern, This research paper is an attempt to present Islam in its true spirit in light of holy Quran and Sunnah. It is hoped that it would pave way for further research in the field.
This study shows that strain of thermotolerant Kluyveromyces marxianus was used for the production of ethanol and invertase (fructofuranosiadse, Ffase). This strain (D-67283) was collected from Shakkar Gunj Sugar Mills, Jhang, selected through Gamma rays on 1.5 % (w/v) deoxy-Dglucose (DG) in liquid medium after growth at 60 °C for 5 days and designated as K. marxianus M15. The selected mutant strain produced maximum ethanol and Ffase at 48 h of cultivation on different substrates including glucose, sucrose, and molasses each at 10, 12, 15 and 17% total sugars in 23 L fermentor (working volume 15 L). Optimized studies on different carbon sources displayed that product formation rate (Qp) was greater on glucose- followed by molasses- medium but was found to be lower on sucrose medium. Product yield (Yp/s) and specific product yield (Yp/x) were also significantly higher on glucose (15%) whereas found as lower on sucrose medium. Specific product formation rate (qp) was also recorded higher on glucose medium and remained lower on sucrose medium. Nitrogen sources like ammonium sulphate, corn steep liquor (CSL), and urea were added to the growth medium to enhance growth and ethanol formation. All these sources were used at the rate to contain 0.11, 0.16 and 0.21 % nitrogen in the growth medium. The best results were observed for fermentation kinetic parameters of growth and product formation by ammonium sulphate using 0.75% (w/v). In further studies, temperature of fermentation was optimized for maximum ethanol production, and substrate utilization, for both wild and mutant strains of K. marxianus. For this purpose, they were grown at different temperatures ranging from 20- 65 oC. The study further revealed that maximum ethanol production on molasses medium supplemented with ammonium sulphate was used 0.75% at pH 5.5 after 48 h at 40 oC. xx Effect of various agitation rates from 250-450 rpm on production of ethanol by K. marxianus cells was carried out and the maximum amount of ethanol produced on the sugar based used was more than 89.96 %. Further increase in agitation intensity did not increase ethanol production in both organisms. Hence, agitation rate of 300 rpm was optimized. The production of ethanol is an anaerobic fermentation process; therefore supply of oxygen to the yeast culture is of great importance as it is needed to support an initial amount of cell mass for maximum ethanol production. At a constant rate of oxygen supply, agitation rate supports uniform distribution of cells and maintain a constant temperature by uniformly stirring the media to dissipate excess heat. Effect of supplying air to the fermentor at different aeration rates (0.25 - 1.5 LL-1min-1) on ethanol fermentation, amount of ethanol produced was 72.5-74.8 gL-1 when the aeration rate was kept at 1.0 LL-1min-1 for 8 h and then between 0.25 - 0.50 LL-1min-1. Further increase in aeration rate, resulted in lower production of ethanol and greater amount of cell mass in the fermented broth but up to a certain extent. The maximum amount of ethanol (75 gL-1) produced when the aeration rate was kept at 0.30 LL -1min-1. Maximum ethanol specific or volumetric productivity increased with the increased temperature up to 40 oC and 45 °C in the case of wild and mutant respectively. The activation enthalpy for ethanol formation (DH* = 55.6 KJ/mol) pathway was lower than that for phytase production ((DH*70-80 KJ mol-1).The estimated values of enthalpy of Ffase formation (13.1 kJ mo-1l) network was lower as compared with the product inactivation (12.0 kJ mol-1 ) network. This usually happens in the case of thermotolerant organisms as reported earlier. Over all these studies revealed that mutant strain acquired significantly better changes in the genetic make up and qualifies for its evaluation at industrial scale ethanol production.