پروفیسر نجیب اشرف ندوی
افسوس ہے کہ پچھلے دنوں ممبئی میں پروفیسر نجیب اشرف ندوی کا اور کراچی میں مفتی انتظام اﷲ صاحب شہابی اکبرآبادی کاانتقال ہوگیا۔پروفیسر اشرف کا اُٹھان بڑاشاندار تھااُنھوں نے معارف میں جو مقالات لکھے تھے اور رقعات عالمگیری کی تحقیق وترتیب اوراُس پرمستقل ایک جلد میں مقدمہ لکھ کر انھوں نے جس قابلیت کا ثبوت دیاتھا اُس کی وجہ سے ان سے بڑی توقعات تھیں۔لیکن احمدآباد اور پھر ممبئی کی آب وہوانے ان کو بجھا سادیا تھا پھر بھی انجمن اسلام اردو ریسرچ انسٹی ٹیوٹ ممبئی کے ڈائرکٹر اورانجمن کے سہ ماہی رسالہ ’’نوائے ادب‘‘ کے ایڈیٹر کی حیثیت سے ان کی علمی وادبی سرگرمیاں کسی نہ کسی صورت میں جاری رہیں۔ چنانچہ اس زمانہ میں ہی انھوں نے ’’لغت گجری‘‘ ترتیب دی تھی ۔ بہرحال اُن کے دم سے اردو زبان سے متعلق تحقیق وریسرچ کا ممبئی میں چرچا تھااور اس بناپر اُن کی وفات علم وادب کی دنیا کاایک بڑاسانحہ ہے۔ [اکتوبر۱۹۶۸ء]
Herbal medicines, complementary or alternative medicines is a wide term for the therapies that are not part of standard care but it has many theories regarding efficacy based on personal experiences, history and common knowledge. It has long been used since ancient times since the beginning of human civilization. Its use had caught much attention in the early 1800s, with the development in the science of chemistry, a new era in pharmacotherapeutics and the use of active chemical ingredients in plants which were known to produce favorable therapeutic effects, were explored, active compounds were extracted, purified and their structure was revealed. This advancement paved the way towards modern pharmaceutical therapy. The modern drugs are based on these herbal medicines, after extracting the active and pure chemical compounds. Pharmacokinetics and physicochemical properties of the active ingredients was explored. It lead to the better understanding of efficacy and safety profile of these drugs and first choice for treatment of various diseases. At the same time, the herbal medicines were considered as secondarily important. After approximately two centuries, the use of herbal medicines have seen a revival globally both in developing as well as developed countries. In the past few years, the practice of using herbal medicines as an alternative and complementary health medicine has gained more importance. Herbal medicines are common for treatment of various ailments including cancer, digestive disorders, pain related disorders, neuropathic ailments and cardiac arrhythmias etc. Even it has been used by pregnant females and mostly perceived as safe. Its use has gained more attraction due to its ‘natural’ approach and lesser side effects. Their use if often overlooked but physicians should pay attention to these medicines. There is lack of familiarity, standardization of the drug components, unproven therapeutic effects in various diseases, unexplored toxicology, pharmacokinetics, drug-drug interactions, and compatibility in patients with varying medical, genetic and demographic history. There are serious concerns regarding the safety, efficacy and quality of herbal products and nutraceuticals. Accidental contamination and deliberate adulteration are assumed to be the main cause of the side effects. Much of the herbal medical knowledge is scattered in different regions of the world and mostly available at family, community and local level and mostly in any native languages. There is need of coherent sources, knowledge, and exploration of these medicines across the world. The herbal medicine has varying diversity in different geological regions and they should be investigated. There should be a regional or national body to control and approve the herbal medicines. Proper documentations on these medicines and food supplements should also be done.
The plant-based medicament isan essential therapeutic weapon to fight against the various human and animal diseases. The present study is based on the rose water obtained from the petals of Rosa damascena Mill available in Pakistan and to correlate its usage and application in personal care preparations. In this study twelve samples of rose water were examined in which eleven samples were collected from the local market / provided by the manufacturer on request, and one hydro distilled sample was prepared on lab scale. The thesis has alienated into five parts. The first part has two portions. Part I (a) consists of the brief introduction related to the rose water botanical source, the region of the world where rose water distilled, chemical constituents, historical, traditional and medicinal importance. In the firstpart, the value of standardization also highlighted through the introduction of standardization, in the end of the first part, the objectives of the present study is discussed. Part I (b) deals with the literature review of Rosa damascena. Rose plant widely disseminated in the plant kingdom and currently consumed bya significant amount as a therapeutic agent. Literature data suggested that rose flowers associated with various biological and pharmacological activities, including anti-cancer, cardiovascular, analgesic, anti-inflammatory, anti-oxidant, anti-microbial and helpful in skin care. Part II describes the experimental work of the present study. Part III shows the results of studies on rose water samples and their physicochemical study, estimation of their volatile components through HS-GC-MS, determination of functional groups through FTIR, toxicity study, in-vitro antioxidant activity study against standard ascorbic acid, invitro anti-inflammatory activities against standard diclofenac sodium, in-vitro sun protection factor determination, anti-microbial activity, bacterial contamination study, cosmaceutical effect on topical application on skin to observe the hydration glow and oil content. Two formulations prepared one is rose water-based cream, study its antiinflammatory and anti-oxidant effects against standard drugs, and the other is rose water based cough syrup and performed its antitussive effect on rats. xxvi In the present study, the pH of rose water is within the range from 4-6 which is an acceptable and non-skin irritating pH value and suitable for topical application. The phytochemical study indicates the presence of flavonoids, tannins, saponins, triterpenoids and fixed oil, whereas the volatile components which have detected through HS-GC-MS are phenyl ethyl alcohol, citronellol, pentadecane, heptadecanol, octadecanol, tetracosane, decane and nonane. FT-IR estimation indicates the presence of carbohydrates, an amine group, a hydroxyl group, alkane, alcohol, ester, ether and carboxylic anhydrides. Toxicity study of rose water indicated that it is non-toxic and LC 50 is more than 3500µg/ml. Invitro antioxidant activity of three samples 12, 9 and 8 shown marked % reduction. The reducing power of sample# 12 recorded as highest 841.66, followed by sample #9 (533.33) and then sample #8 (458.33) against 50µg/ml standard ascorbic acid. The invitro anti-inflammatory activity of rose water studied on four different concentration that is 3,5 7 and 10 ml, and it observed that 10 ml of rose water shown protein inhibition activity equivalent to 100µg/ml of standard diclofenac sodium. Rose water protects from the UV radiation. The sun protecting factor of rose water samples ranges from 3.956 to 0.218. Results of anti-microbial activity indicated that all the test organisms had not shown dose-related sensitivity and thus no zone of inhibition was observed by well diffusion method. The bacterial contamination indicates that rose water samples contain both gram positive and gram negative bacilli. The skin hydration, as well as the glowing effect, significantly increased after the application of rose water. Two formulations are designed based on rose water one is rose water-based cream, and other is rose water based cough syrup. Results of in-vitro anti-inflammatory activity by protein (egg albumin) denaturation of cream formulation throughout the concentration range of 50-1000 µg/ml. For F1 cream formulation was 52.2-80.6 % F2 was 41.4-65.2%, and F3(Placebo) was 0.375-43.67% and for standard drug diclofenac sodium observed within the range of 35.33-86.32%. The IC 50 value of F1 and F2 are 257.39µg/ml and 375.41µg/ml respectively. The reducing power of formulated rose water-basedcream increased with the increase of concentration (25-1000 µg/ml). The reducing power of cream noted as F1 ranges from xxvii 11.80 % to 81.55 %, F2 formulation antioxidant activity ranges from 8.05 % to 72.81 % and F3 formulation which is placebo cream 7.38% to 9.12%ascorbic acid equivalents (AES)µg/g. Four formulations of cough syrup are designed based on rose water and checked their antitussive activity at three different doses (5 ml, 10 ml and 15ml) against standard ivy extract 5 ml/70kg and Dextromethorphan 10 ml/70 kg and itobserved that C1 has the highest inhibition of a cough that is mean±S.E.M (9.00±2.08, 4.33±1.76 and 3.00±0.57) at 30min, 60 min and 90 minutes respectively. Part IV accentuates the overall discussion based on the results of rose water study. Rose water exhibit an anti-oxidant, anti-inflammatory, prevent premature aging, provide nourishment and glow to the skin prevent UV penetration. Rose water cream shows good anti-oxidant and anti-inflammatory activity and rose water based cough syrup has an excellent antitussive effect. Part V has the general conclusion and suggestions for future studies.