اچار نریندر دیوجی
افسوس ہے کہ اچار نریندر دیوجی کی موت سے ہندوستان ایک بڑی شخصیت سے محروم ہوگیا، وہ اپنے اوصاف کے لحاظ سے ہندوستان کے ممتاز ترین لیڈروں میں تھے، سیاسیؔ خدمات، ایثارؔ و قربانی، علمی قابلیتؔ، بے تعصبیؔ، اور شرافت و وضعداری میں اس دور کے کم لیڈر ان کا مقابلہ کرسکتے تھے، وہ پنڈت جواہر لال کے پرانے رفیق اور جنگ آزادی کے نامور سپاہی تھے، اس راہ میں پنڈت جی کے دوش بدوش قیدو بند کی مصیبتیں جھیلیں، اردوؔ، انگریزیؔ، ہندیؔ، اور سنسکرتؔ چاروں زبانوں میں ان کو عبور حاصل تھا، اور ان کے بڑے اچھے مقرر تھے، ان کا یہ کمال تھا کہ جب اردو یا ہندی میں تقریر کرتے تھے تو دوسری زبان کا ایک لفظ بھی نہ آنے پاتا تھا، اودھ کی پرانی راجدھانی فیض آباد ان کا وطن تھا، اس لیے اردو ان کی مادری زبان تھی اور وہ بڑی فصیح و بلیغ اردو بولتے تھے، علمی قابلیت اور تعلیمی تجربہ کی بنا پر لکھنؤؔ اور ہندو یونیورسٹی کے وائس چانسلر مقرر ہوئے، متعدد کتابوں کے مصنف تھے، ان سب سے بڑھ کر ان کی بے تعصبی اور شرافت و وضعداری تھی، ہندوستان میں اس کے جو نمونے رہ گئے ہیں ان میں سے ایک وہ بھی تھے، ایک زمانہ تک کانگریسی رہے پھر اس سے بددل ہوکر سوشلسٹ پارٹی قائم کی، اگر وہ کانگریسی رہتے تو آج مرکزی حکومت کے وزیر ہوتے، مگر انھوں نے اپنے اصول و نظریات کے مقابلہ میں دنیاوی منصب وجاہ کی کوئی پرواہ نہ کی، اب ان کی جیسی شخصیتیں مشکل سے پیدا ہوں گی، اس لیے ان کی موت ایک بڑا قومی حادثہ ہے۔
(شاہ معین الدین ندوی، فروری ۱۹۵۶ء)
Penelitian ini bertujuan untuk mengetahui peningkatan keaktifan dan hasil belajar siswa pada mata pelajaran Pendidikan Agama Islam (PAI) setelah diterapkannya metode pembelajaran demonstration berbasis discussion process. Metode penelitian yang digunakan dalam penelitian ini adalah Penelitian Tindakan Kelas (PTK). Penelitian dilakukan di SMA EKASAKTI Padang pada kelas XI. Metode pengumpulan data yang dilakukan pada penelitian ini adalah: observasi, wawancara, dokumentasi, dan tes. Analisis data dalam penelitian ini dilakukan secara deskriptif kuantitatif dan kualitatif. Hasil dari penelitian ini adalah: (1). Kesiapan siswa dalam menerima pelajaran sebelum dilakukan tindakan 39, 06%, setelah siklus I 70, 31%, dan setelah siklus II 86, 23%. (2). Keaktifan siswa dalam proses pembelajaran sebelum dilakukan tindakan 23, 18%, setelah siklus I 67, 97%, dan setelah siklus II 85, 41%. (3). Persentase siswa yang mendapatkan nilai tuntas di atas 70 sebelum dilakukan tindakan 31, 75%, setelah siklus I 68, 25%, dan setelah siklus II 87, 75%. (4). Terdapat peningkatan yang signifikan antara masing-masing indikator pada setiap siklus, hal tersebut dapat di lihat dari peningkatan kesiapan, keaktifan, dan hasil belajar siswa pada saat sebelum dilakukan tindakan, pada siklus I dan pada siklus II. Pada siklus II semua indikator telah melampaui target persentase indikator keberhasilan yang telah ditetapkan. Sehingga siklus penelitian dapat dihentikan pada siklus II dan dapat di tarik kesimpulan bahwa metode pembelajaran demonstration berbasis discussion process berhasil serta lebih efektif dibandingkan dengan metode ceramah.
This study was conducted in selected areas of Khyber Pukhtoonkhwa Pakistan, namely northern, central and southern regions, with the objective to determine clinico-pathological manifestation of contagious Caprine pleuropneumonia in field outbreak and documented its pathogenesis in experimental animals. The first study included isolation and identification of Mycoplasma species from field outbreaks by usage of a selective differentiating hay flick medium, growth inhibition test and a Polymerase Chain Reaction (PCR) test. Out of 120 inoculated samples, 30% and 22.5% were positive on culture from lungs and pleura. Isolates were identified as Mycoplasma mycoides subspecies capri by a growth inhibition test and PCR. Similarly, tissue samples that were negative on culture were also subjected to PCR analysis. Out of 120 samples 62.5% and 54.16% from lungs and pleura, respectively, were positive. On statistical analysis, a significant difference (P<0.05) was found between results of PCR and culture. This difference reflects that the PCR technique is more sensitive than the culture method. Based upon these findings, disease was prevalent in almost all selected regions of province. The predominant clinical findings include pyrexia, nasal discharges catarrhal initially turned into mucopurulent in the advance stage, excessive lacremation, unilateral and bilateral conjunctivitis with corneal opacity, painful cough, dysponia, weakness, reluctant movement, extended neck, abduction of the elbow and diarrhea. The majority of animals presented pathological lesions in the form of consolidation and marbled appearance of lungs with fibrinopurulent membrane on pleural surface. Straw colored pleural fluid was present in pleural cavity with pleural adhesion, hydro pericardial fluid in pericardial sac, necrotic foci on surface of the liver and pus in the pelvis of kidneys. Histopathological lesions revealed emphysema, atelactasis with interstitial and bronchopneumonia and thickening of interlobular septa with extensive infiltration of polymorph nucleated cells. In second experiment, isolated Mycoplasma mycoides subspecies capri was inoculated into twelve goats to study detailed pathogenesis and usage of immunohistochemical techniques for detection and confirmation of Mycoplasma antigen within paraffin-embedded tissue sections. Almost all animals exhibited signs of disease. Signs of disease appeared in acute septicemic form with fever and nasal discharges on sixth day after inoculation and became more pronounced and severe at by the third and fourth weeks and then progressed to moderate and chronic forms. The pattern of disease development was similar as in a field outbreak, but was more severe in nature. On scoring clinical signs of disease, it presented a specific pattern of infection mild at the beginning and became more severe at the third and fourth weeks and then progressed to moderate and chronic forms. Similarly, gross and microscopic lesions were also recorded in selected organs. In experimental infection, the disease adopted the same pattern of clinical course as in natural outbreak. Four animals were found dead and three developed nervous signs during the course of study. Gross and histopathological lesions were recorded in almost all organs. To demonstrate Mycoplasma antigens in tissues, a special immunohistochemical technique called the labeled streptavidin biotin (LSAB) method was used with hyper-immune serum raised in rabbits against the reference species. Antigen of Mycoplasma mycoides subspecies capri was detected in tissue sections of lungs and lymph nodes. Out of 12 samples, 7 were positive for the immunohistochemical reaction. Mycoplasma antigen was detected in cytoplasm of alveolar macrophages and in the walls of alveoli. This positive result indicated the importance of these cells in host defense mechanism against Mycoplasma. The result also confirmed that the antigen was the same as that inoculated in experimentally infected animals. Samples of all infected goats were found positive by PCR for confirmation of antigens. By comparing results of IHC and PCR, significant difference (P<0.05) was found. This result revealed that PCR is a more sensitive and effective tool for confirmation of the antigen. In conclusion, it was indicated from the present study that CCPP is a wide spread disease in Pakistan, caused by Mycoplasma mycoides subspecies capri and the disease was efficiently iireproduced in experimental animals that adopted acute septicemic form with lethal outcomes. The PCR technique was a more rapid and sensitive tool for diagnosis of CCPP and the immunohistochemical technique was optimized for the first time in Pakistan for detection of antigen within tissues. Key words: Contagious caprine pleuropneumonia, growth inhibition test, PCR, Mycoplasma mycoides subspecies capri, immunohistochemistry, hyperimmune serum