اصغر سودائی(۱۹۲۶ء۔۲۰۰۷ء) سیالکوٹ کے متوسط گھرانے میں پیدا ہوئے۔آپ نے مرے کالج سیالکوٹ سے بی ۔اے کیا ۔طالب علمی کے دوران آپ’’ مرے کالج میگزین‘‘ کے نائب مدیر تھے۔ ۱۹۵۲ء میں جناح اسلامیہ کالج سیالکوٹ میں بطور لیکچرار تعینات ہوئے ۔۱۹۸۴ء میں انھیں ان کی تعلیمی خدمات کے پیشِ نظر ڈائریکٹر آف ایجوکیشن کے عہدے پر ترقی دی گئی۔ آپ کی شہرت اس لحاظ سے بھی ہے کہ آپ تحریک پاکستان کی اساس بننے والے نعرہ پاکستان ،’’پاکستان کا مطلب کیا لاالہ الا اﷲ ‘‘ کے خالق بھی ہیں۔ (۷۳۵)اصغر سودائی کا پہلا شعری مجموعہ ’’شہ دوسرا‘‘ بزم رومی و اقبال سیالکوٹ نے ۱۹۸۹ء میں طبع کیا۔ دوسرا مجموعہ کلام ’’چلن صبا کی طرح‘‘ صدیقی پبلی کیشنز لاہور نے ۱۹۹۹ء میں شائع کیا۔ آپؐ کا تیسرا شعری مجموعہ’’ کرن صدا کی طر ح ‘‘کے نام سے شائع ہو چکا ہے۔ اصغر سودائی کے نظریات ا ن کی اکہتر سالہ زندگی کا نچوڑ ہیں۔ انھوں نے مختلف تحاریک کازمانہ دیکھا اصغر نے تحریکِ پاکستان کی پرزور جدوجہد کو پروان چڑھتے اور پاکستان کو وجود میں آتے دیکھا۔ ادبی تحاریک میں انھوں نے رومانی اور ترقی پسند تحریک کا زمانہ دیکھا۔
ان کے نظریات میں اقبال کے افکار کی جھلک بھی دکھائی دیتی ہے۔ وہ بھی مغرب کی غلامی کو ملتِ اسلامیہ کے لیے باعث ِہلاکت و تباہی قرا ر دیتے ہیں۔ اصغر کے خیال میں اسلام دین فطرت ہے اور اس میں تمام مروجہ علوم کا نچوڑ موجود ہے۔ وہ اسلا م ہی کو انسانی زندگی کی بنیاد سمجھتے ہیں اور اسلام کی صداقتوں او ربین الاقوامیت کے قائل ہیں۔
اصغر سودائی بنیادی طور پر غزل گو شاعر ہیں۔قیام پاکستان کے بعد جن شعرانے میر کے رنگ سخن کی پیروی کی ان میں ناصر کاظمی ،خلیل الرحمان اعظمی اور شہرت بخاری شامل ہیں۔ان شعر امیں اصغر سودائی...
Background: Plant derived medicines are widely used in traditional culture all over the world. Objectives: To determine the effect of Celery Seed Extract (CSE) on uric acid levels in hyperuricemic rats and to compare the effect of allopurinol and CSE. Methods: It was an animal experimental research study. Group A served as negative control whereas Group B served as positive control. CSE was given orally to three groups of rats (C, D, and E). One hour prior to administration of CSE; potassium oxonate was injected intraperitoneally in all groups except negative control to induce hyperuricemia. Similarly, group F was given allopurinol one hour after injection of potassium oxonate. Blood samples were collected for uric acid estimation. Results: It was found that administration of both CSE (group C, D, E) and allopurinol (group F) significantly lowered serum uric acid levels (p<0.001) as compared to positive control (group B). Serum uric acid lowering effect of both drugs CSE and allopurinol was found to be statistically significant on day 3rd and day 7th and was almost comparable. Conclusions: Celery seed extract significantly reduces serum uric acid levels in potassium oxonate-induced hyperuricemic rats and its uric acid lowering effect was comparable with that of allopurinol.
Germin like proteins (GLPs) are member of a large gene family and ubiquitously expressed as plant proteins. The exact mode of action and role in metabolism is not well understood. It is believed that these putative stress proteins are expressed at various developmental stages in response to abiotic and biotic stresses. The present study was designed to clone full length and 5'' abbreviated Oryza sativa Root expressed GLP2 (OsRGLP2) promoter, its genetic transformation into Arabidopsis and characterization. Cloning of full length and 5'' abbreviated OsRGLP2 promoters was carried out by employing GATEWAY™ Technology. Amplification was performed by specific primers designed on the promoter region. Recombinant entry clones were created by using pENTR/D-TOPO® cloning kit. Expression vectors were prepared by employing LR recombination reaction between recombinant entry clones and promoter less destination vector pHGWFS7. Confirmation was carried out by PCR and sequencing. Recombinant expression vectors were named as pNSS-F1 (Full length promoter) and pNSS-F2, pNSS-F3 and pNSS-F4 (5'' deleted promoters). Plant transformation into Arabidopsis was carried out by Agrobacterium mediated floral dip method. T0 and T1 lines were established and transgenic plants were analyzed by molecular and physiological approaches. T1 transgenic lines for each promoter constructs were selected through GUS assay and tested for wound, salt and temperature stresses. Real-time PCR was performed by using two sets of primers, Actin (Housekeeping gene) and GFP (Gene of Interest). Real-time PCR analysis was done by employing 2-ΔΔCt method in which data was normalized by software inbuilt in the real-time PCR system by taking calibrator or untreated sample value xxi as 1. The graphical data shows the times fold increase or decrease in the expression with comparison with untreated control samples. Expression analysis revealed that OsRGLP2 promoter is efficient and specific to wound, salt and temperature stresses. When comparison was done between full length and 5'' deleted promoters, the promoter named pNSS-F3 of 565 bp along with other two larger promoter pNSS-F1 and pNSS-F2 of sizes 1063 bp and 776 bp respectively were responding to all stresses applied during this study. However, when a further deletion was made and shortest promoter pNSS-F4 was created which comprises of 283 bp size, unable to respond against stresses applied. So it can be concluded from present study that during 5'' deletion of full length OsRGLP2 promoter (creation of pNSS-F4), a critical region between P-565 and P-283 on promoter fragment was deleted which contain promoter elements necessary to respond against certain stresses like wound stress, salt stress and temperature stress. While during other deletions (in case of pNSS-F2 and pNSS-F3) in which when the critical part was intact, the promoter responded to applied stresses. It can be further concluded that pNSS-F3 is a good replacement for full length promoter. Due to smaller size of pNSS-F3 promoter (565 bp) and it‟s efficient and specific response against abiotic stresses it can be fused with other promoter and used in combination against certain abiotic or biotic stresses.