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تقلیدِ مُصطفیٰؐ میں اگر سر نہیں گیا
سجدہ اجابتوں کے سفر پر نہیں گیا
اُس کو رہِ حیات میں منزل نہ مل سکی
جو راہِ مصطفی سے گزر کر نہیں گیا
جیسے خدائے پاک کا ثانی کہیں نہیں
نبیوںؑ میں کوئی تیرےؐ برابر نہیں گیا
محشر کے روز بجھ نہ سکی اُس کی تشنگی
لے کر جو پیاس جانبِ کوثر نہیں گیا
تنہا گئے ہیں صاحبِ معراجؐ سوئے عرش
جبریلؑ کا جہاں پر شہپر نہیں گیا
مجھ کو عطا وہؐ کرتے گئے آرزوئے شوق
جب تک کہ میرا کاسۂ دل بھر نہیں گیا
عرفانؔ! جس کو مل گئی خیرالوریٰؐ سے خیر
وہ بھیک مانگنے کبھی در در نہیں گیا
Life of Iqbal is the source of many virtues. This article focuses on some of the sources and motivations that influenced Iqbal's personality. Home environment was the first school of training for Iqbal. Parent's training made him a person of outstanding qualities from the time of childhood. The teachers polished more. Iqbal was also influenced by Sir Syed's movement. This article will give you a glimpse of the research and critique of many new aspects. We must consider the motives of Iqbal's knowledge and wisdom. By the study of these motives, we can examine the mental evolution of Iqbal. Therefore, everything should come to light. Iqbal also expressed his love for nature but within limits. Otherwise, people would make nature an idol and start worshiping it. Authoritative quotes from experts are the part of this research article. These references will provide assistance in the topics of Iqbal Studies.
Fungal endophytes colonize an important niche within the plants through secretion of secondary metabolites. The metabolites and extracellular enzymes produced by endophytic fungi regulate the growth of the host plant and contribute in defence mechanisms.The medicinal plants Caralluma acutangula and Boswellia sacra were used for the isolation of endophytic fungi. The endophytic fungi were identified as Penicillium citrinum, Paecilomyces variotii, Aspergillus nidulans, Fusarium oxysporum, Epicucum nigram, Penicillium purpurogenum, Penicillium spinulosum, Aspergillus caespitosus, Phoma and Alternaria sp. and were assessed for their potential to produce anti-cancerous metabolites by performing MTT assay and extracellular enzymes such as cellulases, phosphatases and glucosidases in growth media. P. variotii, P. citrinum and F. oxysporum showed significantly higher amount of phosphatases and glucosidases as compared to other strains. Additionally, P. variotii and F. oxysporum showed significantly higher potential of indole acetic acid production (tryptophan-dependent and independent pathways). ACC (1-Aminocyclopropane-1-carboxylate) deaminase results showed that P.citrinum, P. purpurogenum and P. Variotii had shown prominent ACC deaminase activity (300 nmol α- ketobutyrate mg-1h-1). Fluorescence-based MUB (4-methyl umbelliferone) standards were used to analyze the presence of extracellular enzymes glucosidase, phosphatase and cellulase. The bioactive secondary metabolites from endophytic P. citrinum also revealed some prominent results by performing MTT assay on breast cancer cell line (MCF-7). The current study concludes that these fungi are producing bioactive constituents that could provide unique niche of ecological adaptation by symbiosis and greatly contributing to the healthy life of their host plant. However, some of the endophytic fungi offer a great potential to produce anti-cancerous metabolites and extracellular enzymes.