آہ! الا ستاذ الاجل
۸؍مارچ کے اخبار الجمعیۃ میں جب یہ خبر نظر سے گزری کہ حضرت الاستاذ مولانا محمد اعزازعلی صاحب پرقلب کادورہ پڑگیا اوراس کی وجہ سے کچھ بے ہوشی رہی اوراب تھوڑی تھوڑی دیرکے بعد دورے پڑرہے ہیں تواسی وقت ماتھا ٹھنکا کہ خدا خیرکرے۔چٹان جب گرتی ہے تو مٹی کے تودہ کی طرح رِس رِس کے نہیں اچانک ہی گرتی ہے۔چنانچہ دوسرے دن کااخبار آیاتو دل کے دغدغہ کی تصدیق ہوگئی اور جس خبرِوحشت اثر کوسننے کے لیے کان تیار نہ تھے اس کایقین کرنا پڑا۔ یعنی حضرت الاستاذ راہی ملک بقا ہوگئے۔اِنّا لِلّٰہِ وَاِنَّا اِلَیْہِ رَاجِعُوْنَ۔
دارالعلوم دیوبند شروع سے معدنِ لعل وگہر رہا ہے۔کتنے ہی ذرے اس کی آغوش میں پلے اور بڑھے اورعلم وفضل کے آسمان پرآفتاب بن کر چمکے، کتنے چاند اورستارے اس کے آسمان پرطلوع ہوئے اوراپنی اپنی روشنی دکھا کراسی دارالعلوم کے دامن میں روپوش ہوگئے، کیسے کیسے گُہر ہائے آبدار اس کی خاکِ پاک سے اٹھے اورعلم وعمل، تقوی وطہارت اورزہد و ورع کی بزم قدس کوجگمگاکر پھرخاکِ لحد میں جاملے ۔آج وہ نہیں ہیں لیکن ان کی یادگاریں باقی ہیں خودان کا وجود فنا ہوگیا لیکن ان کے کارنامے زندہ ہیں اور وہ گویا خود زبانِ حال سے کہہ رہے ہیں:
تلک آثارنا تدل علینا
فانظر وا بعدنا الی الآثار
دارالعلوم دیوبند اگر شاندار عمارتوں ،درسگاہوں،اقامت خانوں اور وسیع و فراخ دروازوں اور اونچی اونچی دیواروں کانام نہیں بلکہ درحقیقت وہ انھیں نفوسِ قدسیہ کاایک پیکرِ محسوس اور انھیں ارواحِ طیبہ کاایک مظہرِ مادی وجسمانی ہے تو کوئی شبہ نہیں کہ حضرت الاستاذ اس عمارت کے ایک اہم ستون اوراس بزمِ انس و قدس کے ایک لعلِ شب چراغ تھے۔گزشتہ نصف صدی میں اس درسگاہ کوتعلیم و تعلم کے اعتبارسے جوشہرت وعظمت حاصل رہی ہے اس میں ایک بڑا حصہ...
Congenital Heart Defect (CHD) is a multifactorial disorder based on both genetic and environmental factors involved in development. The basic problem lies in the structure of heart leading to CHD that occurs in walls, valves, arteries and veins of heart. During cell cycle, the gene that controls this process may mutate, causing disturbance in any portion of heart leading to disturbed blood flow, blood flow in wrong direction or complete blockage. Defect may range from simple with no manifestations to complex with severe symptoms. Simple defects need no treatment while some babies with complex birth defects during birth require special care, vaccination, medication or otherwise treated with surgery. The incidence of CHD has declined from 80 to 20% due to progress in heart surgery techniques, medical treatment and interventional cardiology. Various genetic and non-genetic increase the susceptibility for CHD. The diagnosis and treatment of CHD has greatly improved in recent years. Almost all the children with CHD survive to adulthood and spend healthy and active lives after being treated.
Piper nigrum is a medicinal plant and commonly used as household spice, having piperine alkaloid as major and active ingredient. Piperine is an antibacterial compound and its multiple uses have already been established in literature, therefore it is widely used in folk. There are many other potent antibiotics available in the market that contain substituited-1,3,4-oxadiazole heterocyclic ring structures. The high resistivity of microorganisms towards the prevailing drugs motivated us to synthesize new bioactive molecules by combining the piperine backbone to biological active oxadiazole system. It was assumed that 2,5 – disubstituited-1,3,4- oxadiazole heterocyclic ring may act as potent biological active molecules that boost the pharmacological activities to the new limits. The presented research work comprises the synthesis of some novel multifunctional derivatives followed by structural characterization & biological evaluation. Piperine alkaloid is extracted in lab using soxhlet apparatus, purified, further derivatized and characterized. Piperine, by basic hydrolysis, is converted to piperic acid, which on esterification changed into ethyl piperate and upon reaction with hydrazine lead to piperine hydrazide molecule, which acts as backbone for further derivatization. Piperine hydrazide in three different routes changed to 1-oxadaizole moiety by reacting with CS2 in aprotic solvents, 2- Imine functionality bearing molecules by reacting with various aldehydes, 3-sulphonamide functionalities by reacting with various aryl sulphonyl chlorides. Oxadiazoles further converted to oxatriazole by reacting with hydrazine. Overall we have synthesized 83 derivatives of piperine, with oxadiazole, oxatriazole, amide, imine and sulphonamide functionalities. The compounds presented in this work were synthesized according to available protocols in the literature and has been mentioned in seven schemes in detail (at the end of Chapter 1: Introduction). These synthesized compounds were purified through chromatographic techniques including column chromatography and preparative chromatography. Structures of all these were elucidated through IR, EIMS, 1H and 13C NMR spectroscopic techniques. As literature showed that various molecules containing these type of functionalities are biologically active and used for the treatment of various diseases. This encouraged us to evaluate piperine derivatives to search for more biological active or potent molecules. We have evaluated antibacterial, antifungal, hemolytic and thrombolytic xxvi activities. Some selected molecules from these series were also evaluated for analgesic and anti-inflammatory potential. The antibacterial activity was studied against both gram positive (Bacillus subtilus) and gram negative (Escherichia coli) bacterial strains and also evaluated as antifungal activity against Aspergilus niger strain. We have also investigated their toxicity through hemolytic and thrombolytic activities using blood as a substrate. Analgesic and anti-inflammatory activities for selected compounds were performed using swiss albino mice, which showed very encouraging results. Out of seven prepared series, the series (6a-s) in wihich piperidine ring is replaced with oxadiazole moiety shows most significant results. Two of the derivatives (6b & 6h) from this series (6a-s) found more potent antibacterial activity when compared with diclophenac sodium standard. From same series 6l & 6p showed improved hemolytic activity while 6e & 6f exhibited better thrombolytic activity as compared to piperine.7a-p series have aryl derivatives of oxadiazole-amide functionalities and in this series 7b showed the maximum antimicrobial activity while 7l and 7e of this series exhibited better hemolytic and thrombolytic activities respectively. A minor change in carbon chain for the series 8a-p as compared to 7a-p display overall decrease in activities, however prominent antibacterial and antifungal activities were shown by 8a and 8d. Schiff base containing derivative, 10l showed prominent hemolytic activity among the other members of this series. Sulphonamide bearing derivatives 11a-e showed moderate thrombolytic and hemolytic activity but 11e exhibited maximum antibacterial & antifungal activities. Oxatraizole containing derivative 12b and 12f found to have better thrombolytic activity as well as good antimicrobial agents.Selective derivatives were analyzed against analgesic and anti-inflammatory activities. Addition to Schiff Base moiety to piperine (10h) showed maximum analgesic activity (tail + hot plate method) even higher than Diclofenac sodium standard, while simple oxadiazole (5) and alkyl substituted oxadiazole (6o) showed maximum anti-inflammatory activities.Overall it is concluded that introduction of new functionality to piperine alkaloid made the molecules potent than the parent in most of the cases.