’’ارضِ اقبال آ فاقیت کے آ ئینے میں‘‘ڈاکٹر نصیر احمد اسد کے تحقیقی وتنقیدی مضامین کا اولین تخلیقی مجموعہ ہے ۔اسے ابتدائی کاوش کے نقطہء نظر ہی سے دیکھتے ہیں تو ان کی محنت و لگن اور ادبی ذوق و شوق کی داد دینی پڑتی ہے کہ انہوں نے جس تن دہی سے اس کام کو پایۂ تکمیل تک پہنچایا ہے اسے مدنظر رکھتے ہوئے یہ بات پورے وثوق سے کہی جاسکتی ہے کہ خوب سے خوب کرنے کی جستجو ان کی فطرتِ ثانیہ ہے۔
اس کام کے حوالے سے ایک بات خاص طور پر یہ محسوس ہوئی کہ ان میں سیکھنے کا رویّہ قابل رشک حد تک پایا جاتا ہے اور وہ اپنے آ پ کو بہت کچھ سمجھنے کے خبط سے پاک ہیں اوریہ صرف ادب ہی سے خاص نہیں بلکہ زندگی کا کوئی بھی شعبہ ہو ترقی کی بنیاد یہی رویہ ہے۔چنانچہ جس انداز سے وہ سفر شوق طے کر رہے ہیں امید ہے کہ ایک دن یہ نام ادبی حوالہ ہو گا۔
ان کے مقالات میں تنقیدی اور تقابلی مطالعہ اپنے تہذیبی و ثقافتی پس منظر سے اُبھرتا ہےاور وہ افراد اور رویّوں ہر دو میں اشتراکات اور اختلافات اور مستقبل کے امکانات کو اپنی روایت میں شناخت کرتے اور قدر کا تعیّن کرتے ہیں۔
ڈاکٹر سید عامر سہیل
چئیر مین شعبہ اردو ، اسلامیہ یونیورسٹی، بہاولپور
Allah revealed the Noble Qur'an To His prophet (PBUH) and that is an evidence for the truthfulness of his prophet hood. Allah make a special sequence in this book and he taken the responsibility of its protection, there are scholar whom devoted their lives for the understanding and explanation of the meaning of this Noble book, So All of these Scholar’s explained the verses of Qur'an by their vision, level of understanding, thinking and keeping in view the demands of the place and time. That is the reason that we are finding their different views in the interpretation of Qur'an. In this Article discussed these types of differences and its kinds that we may understand the reality of these various opinions in Tafseer. That these are just the differences in words or there is contradiction in real in their views?
Citrus tristeza virus (CTV) is a plant pathogenic virus belonging to the genus Closterovirus and family Closteroviridae. It is transmitted by vegetative propagation and by several aphid species. It has been reported that CTV has killed millions of citrus trees worldwide. CTV has previously been reported in Pakistan. A survey was carried out in the year 2014 and 1260 random samples were tested from six districts of Punjab and seven districts of Khyber Pakhtunkhwa (KP). Results from DAS-ELISA revealed incidence of 28.3 percent in Punjab and 30.8 percent in KP. Seventy symptomatic samples from both the provinces were collected and tested through DAS-ELISA. The major coat protein coding gene of CTV from forty-eight ELISA positive samples were amplified, cloned into pGEM®-T Easy vector, sequenced and phylogenetic analysis was carried out. Nucleotide sequence analysis revealed 90-100% similarity within indigenous forty-eight isolates, 91.1 to 100 percent similarity with six isolates previously reported from Pakistan and 97.7 to 99.7 percent similarity with T3 USA, VT USA, VT Israel, RB New Zealand, VT India and an Indian isolate of unknown genotype. A maximum likelihood phylogenetic tree indicated that CTV population is diverse in Pakistan with different isolates consisting of one major isolate, T3, and three minor isolates, VT, RB, and VT IND. The most common group (T3 like) is comprised of 42 Pakistani isolates including 36 of our samples and is dispersed all over the country irrespective of the region and province. The remaining three groups are related to VT Israel, VT India and RB New Zealand confined to specific regions. One coat protein gene sequence from the major clade were selected and expressed in E. coli Expression system. The expressed protein was purified and used to raise antibodies in rabbits. Our own produced antisera was tested by DAC-ELISA with the infected and healthy plant tissues. The ELISA readings showed positive results with the infected tissues from Pakistan and USA and negative results with the healthy tissues which confirmed the authenticity of antisera. Results indicated that our prepared antisera is more specific and sensitive against Pakistani CTV isolates as compare to commercially available kit. ELISA readings showed difference in reactivity between the antiserum from two rabbits. No difference in the ELISA readings were observed among five bleeds at the same dilution factor which revealed no difference in the antibody titer among the bleeds. Antisera was also tested against four different dilutions of plant extracts and results showed the sensitivity of one antiserum up to 1/100 of the plant extract. We can use the antisera for the indexing of trees in Pakistan and certification schemes could be introduced to reduce the spread of the virus in the country.