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Evaluation of the antimicrobial activity of the ethanolic and methanolic extracts of plantago ovatta

Thesis Info

Author

Abdul Jabbar

Supervisor

Syed Ali Imran Bokhari; Qasim Hayat

Department

Department of Bioinformatics & Biotechnology

Program

MS

Institute

International Islamic University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Page

83

Subject

Bioinformatics & Biotechnology

Language

English

Other

MS 633.88 ABE

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676722440886

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فضل الرحمن نعیم صدیقی

نعیم صدیقی
یہ خبر رنج و افسوس کے ساتھ سنی جائے گئی کہ جماعت اسلامی پاکستان کے قدیم رکن اور برصغیر کے ممتاز صاحبِ علم و قلم اور اچھے شاعر و ادیب جناب فضل الرحمان نعیم صدیقی نے ۲۵؍ ستمبر کو داعی اجل کو لبیک کہا، انتقال کے وقت ان کی عمر ۸۵ برس تھی۔
نعیم صاحب جماعت کے تاسیسی ارکان اور مولانا سید ابوالاعلیٰ مودودیؒ کے تربیت یافتہ اور معتمد لوگوں میں تھے، جس زمانے میں ترجمان القران پٹھانکوٹ سے شائع ہوتا تھا، اس زمانے سے ان کی نگارشات اس میں چھپ رہی ہیں، مولانا امین احسن اصلاحیؒ وغیرہ کی جماعت سے علاحدگی کے بعد جو لوگ مولانا مودودی کے ساتھ رہ گئے تھے ان میں یہ علمی و ادبی حیثیت سے زیادہ فائق تھے اور مولانا کے بعد سب سے زیادہ تحریری سرمایہ اور لڑیچر ان ہی نے یادگار چھوڑا ہے، جن سے جماعت کا ذہن اور مزاج بنانے میں بڑی مدد ملی۔
معلوم ہوتا ہے کہ مرحوم میں قیادت کا مادہ اور تنظیمی صلاحیت خاطر خواہ نہیں تھی اس لئے وہ نہ جماعت کے عہدوں پر فائز ہوئے اور نہ انہیں تنظیمی ذمہ داریاں سپرد کی گئیں لیکن ان کا شمار جماعت کے فکری رہنماؤں میں ہوتا ہے ان کا ذوق متنوع تھا اور وہ علمی و ادبی ہر طرح کے مضامین لکھتے تھے ایک زمانے میں ترجمان القرآن کا شاید ہی کوئی شمارہ ان کے مضامین سے خالی رہتا رہا ہو، مولانا مودودیؒ کی زندگی میں ان کی حیثیت ترجمان القرآن کے نائب مدیر کی تھی۔ اس کی وجہ سے اور ان کی علمی و تصنیفی قابلیت کی بنا پر خیال تھا کہ مولانا کے بعد وہی رسالہ کی ادارت کی ذمہ داری سنبھالیں گے اور شروع میں یہ ذمہ داری ان کے سپرد بھی ہوئی تھی۔
نعیم صدیقی صاحب کو شعر و...

دعوت الی اللہ کے اصول قرآن کی نظر میں

The word excommunication means to exclude a Muslim from prescribed boundary of Islam. Non of us have the authority of excommunicated any one from Islam except Allah almighty and his beloved prophet Mohammad (PBUH). Because the act of excluding a Muslim, belongs to the orders of Allah (the foundation of Which is Quran) and Sunnah therefore drastic care should be taken in this regard and the one and only would be called as disbeliever if confirmed in the light Quran and Sunnah. Basically any Muslim will be called as Muslim until and unless he is acting openly upon the rules and regulation of Islam. A Muslim would be considered as disbeliever if proclaimed blasphemy intestinally.

Bioassay Guided Isolation and Characterization of Secondary Metabolites from Indigenous Cestrum Species

The work reported here deals with the bio-assay guided isolation of secondary metabolites from Cestrum nocturnum (leaves) and Cestrum diurnum (leaves and berries). First part of the dissertation deals with the biological activities of different parts of the selected plants while second part describes the purification and characterization of the isolated constituents. Shade dried plant material was extracted with methanol. The concentrated methanolic extract was fractionated with n-hexane, chloroform, ethyl acetate and iso-butanol and extractive values determined. All the collected extracts were screened for anti-bacterial, anti-fungal, anti-oxidant and anti-leishmanial activities. The anti-oxidant experiment was also conducted with all the parts. Crude extract of aerial part of C. nocturnum displayed 57 % antioxidant activity while one of its isolated fractions (ethyl acetate) displayed a maximum of 67 % antioxidant activity. Similarly, the crude extract of aerial parts of CD i.e., aerial part without fruit, green fruit, ripe fruit, exhibited 86, 81 and 90 % antioxidant activities respectively. The hexane fraction of CD green fruit showed higher antioxidant potential (87 %) than its crude methanolic extract. Due to our interest in saponins for further studies, they were selectively isolated and processed in the same way for antioxidant activities in crude form from both the plants. The highest antioxidant activity was found with the CD ripe fruit CS extract (90 %) while the least one was with both the aerial parts of the two plants (approx. 80 %). The results obtained with nine microbial strains were moderate. S. typhii was the most resistant strain. On the average gram positive bacteria showed good zone of inhibition with Cestrum diurnum. Gram negative strains showed good results mostly with Cestrum nocturnum. The crude saponins extracts of berries were the most active giving zone of inhibition in the range of 8.5-15mm. Incredible anti-leishmanial activities are being reported for the selected plants. All the plant extracts exhibited hundred percent inhibition of the leishmania parasite. Second part of the thesis deals with the isolation and purification of the secondary metabolites of C. nocturnum and C. diurnum. Hum-V isolated from C. nocturnum was characterised using different spectroscopic techniques as (25R)- spirost-5ene-2,3-diol, [2α, 3β] 3-O-{β-Dglucopyranosyl (1→4)-β-D-glucopyranosyl (1→2)-β-D-glucopyranosyl{(1→4)-β-Dxylopyranosyl] (1→4)-β-D-galactopyranoside). It was reported as nocturnoside A. Another reported compound was also purified and characterized as karatavoiside A, spirost-5-ene-2,3-diol [2α, 3β,25R], 3-O- [β-D-glucopyranosyl(1→2)] {-β-Dxylopyranosyl( 1→3)-β-D-glucopyranosyl(1→4)}-β-D-galactopyranoside. A new compound isolated from the same specie, elucidated as 3-O-β-D-xylopyranoside-olean- 12-en-28-oic acid-28-O-β-arabinopyranosyl-(1-3)-β-D-galactopyranosyl-(1-2)- β-Lglucopyranosyl-( 1-4)- β-L-glgucopyranosyl ester.