غلام غوث غلامی (۱۹۴۰۔۱۸۴۷)میترانوالی سیالکوٹ میں پیدا ہوئے۔انھوں نے ملا عبد الحکیم سے ادب وفقہ کی تعلیم حاصل کی۔ آپ نے فردوسی کے شاہنامہ کے مقابلہ میں سیالکوٹ کے ایک مشہور راجہ سا لباہن کی مختصر مثنوی لکھی ہے۔ آپ کی تالیفات قریش (خود نوشت سوانح) ‘ فرہنگ‘ منتخبات ‘ عربی بہ فارسی سوانح حضرت بلالؒ (منظوم) ‘خزینہ کرامات‘ سفر نامہ مجاز‘ فیروز نامہ حجاز‘ تلبیس ابلیس‘ زہدتہ القواعد در علم صرف‘ تازیانہ‘ غفلت ‘ درعیوب ہندوستان اور انشا غلامی ہیں جو بہت کوشش کے باوجود بازیاب نہیں ہو سکیں۔(۳۰)
۳۰۔ غلام غوث چیمہ ، ’’مولوی غلام غوث غلامی‘‘ شمولہ ’’الابصار‘‘ ڈسکہ‘ گورنمنٹ ڈگری کالج‘ ۲۰۰۳ء ‘ ص ۲۷۰۔۲۷۱
Congenital anomalies are a major health problem all over the world; especially it is important cause of deaths and birth defects, chronic illness and disability in infants. The major cause of this is consanguineous marriages. Generation of cousin marriages have significant association with congenital anomalies Objective: To find out the association of consanguineous marriages with congenital anomaliespresent at the time of birthMethods: A cross sectional study was conducted at District Head Quarter Hospital, Okarafrom May to August, 2018. 100 adult individuals aged between 19 to 55 years, with and without cousin marriage of both genders were consecutively enrolled. Participants were assessed through pre-tested questionnaire, with prior written informed consent. Unwilling married individuals and individuals from other hospitals were not selected Results: According to resultsthere was a significant association between generation of cousin marriages with congenital anomalies present at the time of birth, as p value was 0.002Conclusions: Study concluded that the generation of cousin marriages has significant association with congenital anomalies present at the time of birth and due to cousin marriage 59% of the couples had congenital abnormalities in their children and 85% had genetic disorders.
Disease associated risk factors in premenopausal women includes professional and household stresses. While in post menopausal women the major factor is sedentary life style. Physiological characteristic have shown that the major risks associated with the aggressiveness of tumor are the receptor status especially triple negative (ER, PR and her2/neu) in both premenopausal and post menopausal women. Techniques of ELISA SEC, HPLC were tried to find out the method for estimation of the level of MUCI/CA15- 3 that showed an early detection and that was also economical. Computational protein modeling was used to predict secondary structure of MUC1. Predicted secondary structure of MUC1/CA 15-3 contains 6.0% helix, 7.9% strand or β-pleated sheet and 86.1% loop. Percentage composition shows an increased number of alanine, proline, serine, tyrosine, valine and glycine in helix, strand and turn or loop structure that indicates its basic and hydrophobic characteristics. MUC1/CA 15-3 comprises of an extracellular domain and a membrane associated domain. A junction of 3 amino acid units i.e. Cys-Gln-Cys between the extracellular subunit and transmembrane subunit is also observed. This motif is necessary for surface expression. The extracellular subunit consists of a tandem repeat unit that consist 20 amino acid residues i.e sapdnkpags and tappahgvts. This region of MUC1 may be used as vaccine for breast cancer. The subcellular subunit of MUC1 mainly consists of immunodominant DTR motif at 922 postion of the domain, is haeavily glycosylated and may protect the cell from any of the foreign body or tumor cell. The other subunit of MUC1/CA 15-3 consists of 17 amino acid residues. This subunit is associated with the plasma membrane. xixPost translational modifications (glycosylation, phosphorylation and myristoylation) are mainly observed in extracellular subunit. Several phosphorylation sites, Myristoylation sites and some glycosylation sites of tandem repeat region were predicted in MUC1/CA 15-3. It was observed that due to these modifications, the protein performed different functions. Alignment of the sequence of MUC1/CA 15-3 with the other family member of mucins (MUC2- MUC12) shows highest homology of MUC1/CA 15-3 with MUC4. The property of breast tumor to produce mucinous antigen was used for the synthesis of vaccine using the experimental mice. Electrophoretic technique was used to confirm the production of antibody that may be effective against antigen of MUC1/CA 15-3. The conclusion is that CA 15-3 is a transmembrane glycoprotein having basic and hydrophobic charecterstics. The most important part of this protein is extracellular and transmembrane subunits. These subunits of protein undergo posttranslational modification and are important in immunization and vaccine formation.