الحاج مولوی عین الحق اعظمیؒ
افسوس ہے کہ ۹؍ اگست کو الحاج مولوی عین الحق اعظمی کانپور میں رحلت فرماگئے، اناﷲ وانا الیہ راجعون۔ وہ ۱۵؍ فروری ۱۹۱۳ء کو اعظم گڑھ کے ایک گاؤں میں جواب ضلع مؤ میں ہے پیدا ہوئے ابتدائی تعلیم گھر پر ہوئی مولوی کرنے کے بعد انٹرنس پاس کیا اور ۱۹۳۴ء سے اعظم گڑھ میں چمڑے کی تجارت شروع کی جس میں اﷲ نے برکت دی مگر ان کی حوصلہ مند طبیعت اس پر قانع نہیں ہوئی اور ۱۹۵۹ء سے کانپور بھی ان کی جولانیوں کا مرکز ہوگیا، یہاں جاجمؤ میں انھوں نے نیولائٹ ٹینری کی داغ بیل ڈالی اور جب کاروبار میں زیادہ وسعت و ترقی ہوئی تو ۱۹۶۷ء میں یہیں توطن اختیار کرلیا، اب یہ کاروبار اتنا بڑھ گیا ہے کہ ان کے پانچ بیٹے شب و روز اسی میں لگے رہتے ہیں۔
علم و تعلیم سے ان کو بڑا شغف تھا، اکثر دینی مدارس کی مالی امداد کرتے تھے خود مدرستہ الاسلام کے نام سے اپنے گاؤں میں ایک مدرسہ قایم کیا، جس کے سالانہ جلسوں میں مولانا امین احسن اصلاحی اور دوسرے مشاہیر شریک ہوتے تھے، جاجمؤ میں انھوں نے لڑکیوں کی تعلیم کے لیے جامعۃ الزہرا قائم کیا۔
دارالمصنفین کے علاوہ اعظم گڑھ میں علامہ شبلیؒ کی یاد گاریں مدرستہ الاصلاح سرائمیراور شبلی کالج بھی ہیں،تینوں اداروں اور ان کے ذمہ داروں سے حاجی صاحب مرحوم کے روابط تھے، دارالمصنفین میں ان کی آمد و رفت ۱۹۳۵ء سے شروع ہوگئی تھی انھوں نے اس کی فصل بہار دیکھی تھی، علامہ سلیمان ندویؒ اور مولانا عبدالسلام ندویؒ کی مجلس علم و ادب میں بھی وہ باریاب رہ چکے ہیں، کانپور میں مستقل سکونت اختیار کرنے کے بعد بھی جب وہ اعظم گڑھ آتے تو یہیں قیام کرتے مجھ پر ان کا خاص لطف و کرم رہتا، جب...
Dengue Hemorrhagic Fever (DHF) is a disease caused by a dengue virus infection which is transmitted through the bite of the Aedes aegypti mosquito. To prevent the occurrence of dengue cases, it is necessary to eradicate mosquito nests. The market is one of the public places that has a risk of dengue transmission. This study aims to determine the density description of Aedes aegypti larvae based on the knowledge, attitudes and actions of cleaning managers, traders and visitors to Pasar Daya Makassar City. This type of research is descriptive observational. The research samples were cleaning managers, traders and visitors as well as containers at Pasar Daya and at the respondent's house. Sampling for respondents using purposive sampling method using inclusion and exclusion criteria. The results showed that the number of containers positive for ae. Aegypti larvae in the Daya market was 34 containers with a container index value (CI) = 35.4% and the value of Density Figure (DF) 8 and the presence of Ae. Aegypti larvae in the respondent's house (positive) amounting to (51.4%). At the level of knowledge of respondents in the poor category (68.6%), while the attitudes and actions of respondents in the poor category showed a high enough percentage, namely 65.7%, and (68.6%). Based on the results of this study, it can be concluded that the density of Aedes aegypti larvae in Pasar Daya Makassar City is in the high category.
The aim of the present investigation was to determine the effect of salt stress on the physiology of twelve genotypes of tomato (Solanum lycopersicum L.), to study the genetic diversity among these genotypes and to transfer salt tolerant gene in the selected genotype through Agrobacterium mediated gene transformation method. The seeds of different tomato genotypes were obtained from Ayub Agricultural Research Institute (AARI), Faisalabad and from Horticulture Research institute (HRI), NARC, Islamabad. First experiment comprises study of plant growth, ionic content, ABA content of shoot and root and proline content at four different NaCl salinity level. The genotypes Avinash, NTH242 and Gol performed better at higher salt concentration as indicated by less inhibition of plant height, shoot and root fresh weight and shoot and root dry weight. There was less translocation of Na + to shoot but greater accumulation of K + and Ca 2+ concomitant with higher production of osmoregulant (proline) and endogenous ABA accumulation in shoot was recorded. Second experiment was conducted to study the genetic diversity among the twelve tomato genotypes through randomly amplified polymorphic DNA analyses. Twelve tomato genotypes were grouped in four clusters at similarity coefficient value of 0.88 on the basis of randomly amplified polymorphic DNA analysis. Cluster-I consisted of a single tomato genotype “NTH-242”. Cluster-II of three tomato genotypes Lyp No.1, BARI, Roma and Cluster-III grouped Pakit and Riogrande together. Cluster-IV composed of maximum tomato genotypes i.e. 88572, Gol, Nagina, Checo, Pant Bahar and Avinash. Third experiment deals with Agrobacterium mediated transformation of DREB 1A gene in tomato using tissue culture. Prior to transformation three tomato genotypes were subjected to tissue culture studies to select the best genotype for callus induction and regeneration. Hypocotyls and leaf discs were used as explant source and inoculated on Murashige and Skoog media having different hormonal combinations. The callus induction and regeneration frequency varied among genotypes and it was concluded that hypocotyls of tomato genotype Avinash produced maximum callus induction and regeneration frequency on MS media xiiisupplemented with IAA (0.5 mg/l) and Kin (1.5 mg/l). Agrobacterium mediated gene transformation was carried out in tomato genotype Avinash to transfer DREB 1A gene tolerant to salt stress. During the study it was observed that two min infection time with Agrobacterium, two days for co-cultivation, Acetosyringone @ 50 μM in co-cultivated media and 50 mg/l hygromycin in selection media was optimum for high frequency transformation. Polymerase chain reaction (PCR) analysis was conducted to confirm trasfomants by amplification of 500 bp size DNA fragment for hygromycin resistance gene.