نواب مقصود جنگ مولاناحکیم مقصود علی خاں
اسی طرح کادوسرا حادثہ نواب مقصود جنگ مولانا حکیم مقصود علی خاں صاحب کاپیش آیا۔ مرحوم ایک طبیبِ حاذق، ممتاز عالمِ دین اور بہترین خطیب و مقرّرتھے۔زندگی کابڑا حصّہ حیدرآباد میں بسر کیااورکوئی شبہ نہیں کہ بڑی شان سے بسر کیا۔ ہوش مندی، معاملہ فہمی، صاف گوئی،جرأتِ حق اورپاسِ وضع میں اپنا جواب نہیں رکھتے تھے۔نظام دکن کے طبیبِ خصوصی اورمصاحبِ خاص ہونے کے باوجود حیدرآباد کی عوامی زندگی میں بھی پوری طرح دخیل تھے،ہراجتماعی کام میں بڑھ چڑھ کرحصّہ لیتے تھے اور ہرطبقے میں اُن کی رائے کاوزن محسوس کیاجاتا تھا یہی وجہ ہے کہ ریاست کے ختم ہونے کے بعد بھی اُن کے مقامِ عظمت میں کوئی فرق نہیں آیاتھا۔عمر بھر طبِ یونانی کی بے لوث خدمت کرتے رہے، جہاں تک فن کاتعلق ہے سچ تویہ ہے ان کی سرگرمیوں سے اس فن کے تنِ بے جان میں روحِ تازہ آگئی تھی، حیدرآباد کاطبیّہ کالج اور انجمن اسلامیہ اُن کی زندگی کے شاندار تعمیری کارنامے ہیں اور جب تک یہ ادارے قائم ہیں اُن کے جذبۂ خدمتِ خلق پرگواہی دیتے رہیں گے۔ ’’دارالعلوم دیوبند‘‘ ’’جمعیۃ علماء ہند‘‘ اور ’’ندوۃ المصنفین‘‘سے بھی ربطِ خاص رکھتے تھے۔ پیرانہ سالی ،ضعیفی اور معذوری کے باوجود طویل سفر کی صعوبتیں برداشت کرتے تھے اور دارالعلوم کی مجلسِ شوریٰ کی کارروائیوں میں جوانوں کی طرح حصّہ لیتے تھے اوراُن کے تجربے ،خلوص اور حُسنِ تدبّر سے بہت سے نازک اور اُلجھے ہوئے مسئلوں میں مدد ملتی تھی۔
۱۹۵۰ء میں حیدرآباد میں جمعیۃ علماء ہند کاجو تاریخی اجلاس ہواتھا اس کی کامیابی مرحوم ہی کی جدّوجہد اوراثر ورسوخ کی رہینِ منّت تھی،صدر استقبالیہ کی حیثیت سے مرحوم نے اس اجتماع میں جو خطبہ پڑھا تھااُس سے ان کے علمی پایہ اور سیاسی بصیرت کابخوبی اندازہ ہوسکتا ہے۔
راسخ العقیدۃقدیم عالم دین ہونے کے باوجود...
Hazrat Abdul Rehman (may Allah be pleased with him) belonged to Arab tribe of Quraish and was a close relative of Mohammad (peace be upon him). At the time of conquest of Makkah He (may Allah be pleased with him) entered the circle of Islam. He (may Allah be pleased with him) is counted among the companions of Muhammad (may Allah be pleased with him) who came to sub-continent specially Balochistan in order to preach for Islam and Jihad during the Khilafat of orthodox caliphs. He (may Allah be pleased with him) came to Balochistan twice for Jihad and conquests first during the Khilafat of Hazrat Usman (may Allah be pleased with him) and second time in the early era of Hazrat Muawia (may Allah be pleased with him). He (may Allah be pleased with him) played a vital role in the wars of Balochistan. He (may Allah be pleased with him) established Zehri his abode and capital after conquering Kalat, Khuazdar (Sajistan), Kachi, Gandhava, and Chaghi, and from here he expanded the series of his conquests till Kabul and Qandar. Besides this, he included many areas of sub-continent in the Islamic empire of conquered areas. His (may Allah be pleased with him) life is consists of great chapters of sincerity in deeds. Wisdom and valor, determination fearlessness, strife, hospitality, simplicity and patience. He (may Allah be pleased with him) is counted among the great generals of Islam had the honour to have carried the message of Holy faith in every corner of Balochistan in tough and unfavorable conditions and planted the flag of Islam in Balochistan forever.
The present study was carried out to clone a glycoside hydrolase GH 13 family enzyme from Thermotoga petrophila into Escherichia coli and characterization of the recombinant enzyme. After amplification of the GH 13 family gene of Thermotoga petrophila, it was cloned in E. coli DH5α by using pTZ57R/T as a cloning vector. Screening of positive clones was performed by colony PCR, double digestion of recombinant pTZ57R/T containing GH 13 family gene with NdeI and HindIII as well as by sequencing of cloned gene. Expression of GH 13 family gene was checked in E. coli BL21 (DE3) by using pET 21a (+) as an expression vector. The growth conditions i.e temperature, pH, effect of IPTG and time of induction and optical density of culture at the time of induction were optimized for maximum expression of GH 13 family gene. Various other fermentation parameters like size of inoculum, agitation rate, effect of different media, aeration rate and dissolved oxygen were also studied for maximum expression of cloned gene. Purification of the recombinant GH 13 family enzyme was carried out by heat treatment followed by ion exchange chromatography with 34.6-fold purification having specific activity of 126.31 U mg−1 and a recovery of 56.25 %. Molecular weight of the purified GH 13 family enzyme, 70 kDa, was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was stable at 100 °C temperature and at pH of 7.0. The enzyme activity was increased in the presence of metal ions especially Ca+2 and decreased in the presence of EDTA indicating that the α-amylase was a metalloenzyme. However, the addition of 1 % Tween 20, Tween 80 and β-mercaptoethanol constrained the enzyme activity to 87, 96 and 89 %, respectively. No considerable effect of the organic solvents (ethanol, methanol, isopropanol, acetone and n-butanol) was observed on enzyme activity. Line-weaver Burk plot showed Km and Vmax values of 12.35 mM and 25.839 U/ml/min, respectively. Thermodynamic parameters for hydrolysis of soluble starch were found to be Ea=28.445 KJ/mol, ΔH= 34.12 KJ/mol, ΔS= -6.7 KJ/mol and Q10=0.47. Conserved domain analysis of GH13 family protein showed that it it comprises of three conserved domian: AmyAc_MTase (maltosyltransferase), domain of unknown function and AmyA (glycosidase). Homology modelling of GH13 family gene was carried out using 2 templates 1gjuA and 4gkl.1. Enzyme-substrate docking of GH 13 family enzyme was carried out by using maltotriose and dextrin as substrates. 76% desizing of cotton cloth with purified recombinant GH13 family enzyme was achieved at optimized conditions (with 150U/ml enzyme in a buffer of pH 7.0 at 80ºC after 60 min of incubation). In the light of all results obtained in this study it is concluded that this recombinant GH13 family enzyme could be used as beneficial candidate for textile industry.