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Isloation, molecular characterization and gene annotation of DREBIA gene from Arabidopsis using bioinformatics tools

Thesis Info

Author

Rashid , Faiza Abdul

Supervisor

Naveeda Riaz

Department

Department of Bioinformatics and Biotechnology

Program

BS

Institute

International Islamic University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Bioinformatics & Biotechnology

Language

English

Other

BS 583.123 FAI

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676723222954

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ہجر فراق سوہنے یار دے وچ: ۲

سی حرفی ۔۲
(ہجر فراق سوہنے یار دے وچ)
الف
آماہی ترے ہجر اندر باہیں سرے تے رکھ کے رُنیاں میں
برہوں مار گھتی، دکھاں ساڑ سٹی، پاڑ سٹیاں چولیاں چُنیاں میں
پھٹ گُھل دے نہیں جدائی والے، کونج ڈار دے وچوں وچُھنیاں میں
آتش عشق حنیف چا ڈنڈ چاہڑی، جگر دل دیاں بوٹیاں بھُنیاں میں

ب
بہت واری سمجھا رہیاں، اکھیں ویکھنوں باز نہ آئوندیاں نی
دنیا جگ جہان بھلا سارا، لاگھا یار دا مل کھلوندیاں نی
اکھیں، اکھیں نوں ویکھ کے رہن راضی،اکھیں لا پریت نبھائوندیاں نی
حنیف یار محبوب دے ویکھنے نوں، اکھیں بنھ کے ٹور لیائوندیاں نی

ت
تانگھ محبوب پیاریاں دی، جیو ڈاہڈڑا آن اداس ہویا
ہجر ساڑ دتا، دکھاں مار دتا، بھج وانگ کباب دے ماس ہویا
درداں مشک پکائی وچھوڑیاں دی، دل ڈھگیاں وانگ خراس ہویا
دے درشن آن کے عاجزاں نوں، نہیں کدی حنیف دے پاس ہویا
ث
ثابت قدم جے عشق ہووے، سمجھو ٹاکرے نال تقدیر ہو گئے
ہک وار ڈٹھا اوہدا رخ روشن، قیدی عمر دے باہجھ تقصیر ہو گئے
ہک وار ڈٹھی تصویر جنہاں، اوہ نال تصویر، تصویر ہو گئے
ریت عشق دی رہی حنیف اینویں چھڈ بادشاہ، تخت فقیر ہو گئے

ث
ثبوت منگے وفا ساریاں دا، آپ وفا دا نام نہ جاندا ای
سانوں ہجر فراق وچ پا کے تے، آپ نال نازاں موجاں ماندا ای
وقت جدوں وچھوڑے دا آ جاوے، ناز مان نہ رہوے کوئی مان دا ای
حنیف ہتھ اٹھا دعا منگے، تنبو رحمتاں دے رب تان دا ای

ج
جان جہان قربان ہر شے، سوہنے یار دی شیریں زبان اتوں
لباں لال گلاب دا پھل مہکے، کراں صدقے جان دہان اتوں
حسن بے پرواہ، پرواہ ناہیں، عاشق وار دے جان اس جان اتوں
دھوکا نال حنیف دے کریں...

شاہ ولی اللہ كا نظریہ اخلاق و معاشرت

His distinction was that he reflected over the Islamic system and applied it upon the modem age. He proved that the teachings of Islam cover not only faith, beliefs and rituals of worship but also rule all the aspects of social and moral values of a Muslim. He proved with profound arguments that these fundamental principles prevail and govern the Muslim society and its ethics. Shah Waliullah had a broad vision and a versatile approach towards the different aspects of political, moral and social behaviors. The most important thing about his contributions is that his views are widely accepted and welcomed by the scholars who came after him.

Efficacy of Entomopathogenic Fungi and Their Combinations With Some Selective Insecticides Against Bemisia Tabaci Genn. and Tetranychus Urticae Koch on Cotton

The cotton whitefly, Bemisia tabaci Genn. and Two-Spotted Spider Mites (TSSM), Tetranychus urticae Koch are emerging as important sucking pests of cotton crop. The indiscriminate use of pesticides against insect pests have resulted in the development of resistance among these pests as well as environmental and health hazards. The use of microbial control agents is an important approach to combat this menace. The present study was therefore, carried out to determine the efficacy of some strains of entomopathogenic fungi viz., Metarhizium anisopliae, Isaria fumosorosea, Lecanicillium muscarium and Beaveria bassiana against all immature stages of whitefly and eggs and adult females of TSSM in the Department of Entomology at University of Agriculture, Faisalabad, Pakistan and Department of Pure and Applied Ecology, Swansea University, Swansea U.K. for the bioassays on whitefly. The potential of various entomopathogenic fungi was evaluated on 2 nd instar nymphs of whitefly and four of them were found promising. The detailed studies were carried out on other immature stages of whitefly with the promising strains. The dose- mortality response was observed by using serial dilutions, 1×10 5 to 1×10 8 conidia mL -1 against most susceptible stage using detached leaf immersion method for whitefly and spraying the detached leaf for bioassay with TSSM. All tested fungal isolates caused mortality in the tested stages of both insects under consideration except eggs of whitefly for which very low susceptibility was observed. The L. muscarium (V17) isolate was highly virulent among the tested ones for whitefly nymphs while Pf (n32) isolate was highly virulent against both the stages of TSSM. The mortality of each stage was recorded daily up to 8 days for both the insects under study. The % mortality significantly differed based on fungal isolates, stage of the insect and conidial concentrations. The % mortality increased with increasing the dose of conidial concentration of each fungal isolate in each insect. For whitefly, the most virulent isolate, (V17) has LC 50 value of 1.9×10 5 conidia mL -1 and it killed about 89.58 % of the treated second instar nymphs 8 days post treatment while its LT 50 value at higher concentration is 4.03 days. For TSSM, the most virulent isolate was Pf (n32) with lowest LC 50 value of 9.1 × 10 4 conidia mL -1 on 8 th day and LT 50 value of 4.58 days at 1×10 8 conidia mL -1 . It killed about 82.16 % of the inoculated females 8 days post treatment. The xresults indicated that L. muscarium and I. fumosorosea strains have potential as microbial control agent against cotton whitefly and mites, respectively, in Pakistan. The studies were also carried out to determine the LC 50 values and also efficacy of some commercial insecticides and acaricides against whitefly and TSSM in the laboratory. Four different insecticides were evaluated in the lab against 2 nd instar nymphs of whitefly on cotton. All the tested insecticides caused significant mortality of whitefly from 3-5 days post treatment. However, the most effective insecticides for whitefly, up to 5 days were Mospilon ® and Confidor ® with very low LC 50 values while others were also equally effective but with high LC 50 values. Similarly, the chlorfenapyr 36 SC, pyridaben 15 EC and fenpyroximate 5 SC proved to be the best miticides among the tested ones for the control of TSSM based on their LC 50 values. The nine commercial pesticides and their effects on germination of conidia and mycelial growth of highly efficacious fungal strains viz., I. fumosorosea and L. muscarium were investigated in vitro. There was a significant variation in conidial germination as well as mycelial growth by all tested pesticides depending upon the dose of pesticide and type of fungus. Among the pesticides tested azocyclotin was proved to be highly toxic to germination of spores as well as mycelial growth followed by pyridaben, acetamiprid and propergite while buprofezin was the least toxic. Isaria was proved to be more sensitive at field recommended doses than Lecanicillium strain to all pesticides. Generally, among all pesticides used the insecticides were more compatible to fungi than acaricides although these also inhibited germination of spores and fungal growth to some extent. The combined application of pesticides with selective fungi proved to be synergistic giving more control than used alone. The combined effects were tested under greenhouse conditions on potted plants. Buprofezin, acetamiprid, imidacloprid and diafenthuron were used against 2 nd instar nymphs of whitefly while chlorfenapyr, pyridaben and fenpyroximate were used against TSSM. Only buprofezin and imidacloprid insecticides showed synergistic effects and others additive while no one showed antagonistic results. Chlorfenapyr combined with fungus yielded highest mortality among acaricides tested. However, further investigations under field conditions may reveal more comprehensive results.