ڈاکٹر وھبۃ الزحیلی کے تفسیری آراء کی روشنی میں اسلام کے عادلانہ نظام کا تحقیقی جائزہ

It is an admitted fact that Islam is “Universal Din” and a complete code of life. Its universality and conciseness is proved from Quran itself. Quran identifies the universality and surmounts it upon all over other Ady┐n and says, “And He sends his messenger along with righteousness and fait Din-e- ╓aq, so that surpass it upon other Dins, though it will be unpleasant for the polytheists”. The Holy verses shows and argues that Dine- Islam is a superior to all other Dins, it may be through love, arguments, conclusiveness or through state and governed on its completion Quran says, “Today I completed your “Din” for you along with all the blessings and liked Islam as a Din for you”. In a nutshell, the above two mentioned the Holy verses indicate clearly the universality and comprehensiveness, because the “Din” which will be superior and must be universal and precise. Islam is the only religion which is beneficial for all mankind in each and every aspect. Its universality is declared that it is a surety for mankind prosperity. Allah says in His Holy Book, “The Holy Quran” that do justice as it is more nearer to piousness. Allah has described “Justice twenty six times His Holy Book and it is also among one of His qualities. All these show the importance of justice.

Characterization of Ligninolytic Enzymes Produced by Schyzophyllum Commune in Solid State Cultures for Industrial Applications

The potential of an indigenous white rot fungus Shyzophylum commune IBL-06 for the production of ligninolytic enzymes in solid state fermentation of banana stalk was investigated. The production process was further improved by optimizing some physical parameters (incubation time, moisture level, pH, temperature, inoculums size) and nutritional factors (carbon and nitrogen sources, carbon: nitrogen ratio, mediators and metal ions). By optimization of different parameters the maximum activities of enzyme synthesized by S. Commune IBL-06 were 3745 IU/mL of MnP, 2700 IU/mL of LiP and 345 IU/mL of Laccase after 3 days incubation at рH 4.5 and 35°C temperature with inoculum size, 3mL; moisture content, 60%; C: N ratio, 20:1(glucose and ammonium nitrate as carbon and nitrogen supplements), 1mM MnSO4 as mediator, 1mL and 1.25mM MgSO4 .7H2Oa, 1mL. The enzymes produced under optimum conditions were purified by (NH4SO4)2 precipitation, dialysis and Sephadex G-100 gel filtration chromatography. The purified enzymes were run on SDS-PAGE and characterized through kinetic studies. The purified MnP was a monomeric protein with mass of 40 kDa. The optimum pH and temperature for MnP were 5 and 40°C with 0.29 mM KM and 450mM/min Vmax using MnSO4 as substrate. The enzyme was activated by 1mM CuSO4 but was inhibited by CaCl2, EDTA, TEMED, β- Marcaptoethanol, AgNO3 and Pb(NO3)2. The molecular weight of purified LiP was 43KDa and it displayed a single band on SDS-PAGE. LiP showed optimum pH 5.0, optimum temperature, 35°C; KM, 0.5 mM and Vmax, 400 mM/min using varatryl alcohol as substrate. The enzyme was inhibited by CuSO4, MnSO4, CaCl2, EDTA, TEMED, β-Marcaptoethanol, AgNO3, Pb(NO3)2. Molecular mass of Laccase was 63 kDa and it had optimum рH 6.0, optimum temperature 40°C, KM value 0.25mM and Vmax 80mM/min using ABTS as substrate. The laccase activity was enhanced by 2mM CuSO4, and was inhibited by MnSO4, CaCl2, EDTA, TEMED, β-Marcaptoethanol, AgNO3, Pb(NO3)2. Crude ligninase extract decoulorized Novasol direct blue dye to 80%, followed by Novasol direct yellow dye to 60%, Novasol direct red to 38% and Novasol direct black to 37%. The effluent from Magna textile industry was maximally decolorized to 87% in 24 hours, followed by effluents from Crescent, Arzoo and Chenab textile industries. S. Commune IBL-06 produced high activities of MnP and LiP having higher catalytic activities as compared to most of the previously reported enzymes.