پنڈت وجے لکشمی
اسی ماہ میں ایک دوسرا حادثہ ٔ جانکا ہ ملک کوپنڈت وجے لکشمی کابھی پیش آیاہے۔ محترمہ جواہرلال نہرو کی ہمشیرہ تھیں۔لیکن وہ خودبھی بڑی قابلیت کی مالک تھیں۔ صحیح بات کہنے میں وہ کبھی نہیں چوکیں، چاہے اس کے لیے ان کو اپنے خاندانی افراد ہی سے ناراضگی کیوں نہ لینی پڑی ہو۔ایمرجنسی کی مخالفت میں وہ اپنی بھتیجی شریمتی اندرا گاندھی کے خلاف میدان میں کودپڑیں۔
وہ بڑی بہادر خاتون تھیں اورایک اچھی مقرر بھی تھیں۔ بھارت کی نمائندگی کرتے ہوئے انہوں نے غیر ملکوں میں بھی اپنی قابلیت کالوہا منواتے ہوئے اپنے ملک کانام روشن کیا۔یو۔این۔ او میں ان کی صلاحیتوں کابرملا اعتراف واظہار کیاگیا۔ان کے انتقال سے ہندوستان نے اپنی ایک بڑی محسنہ کوکھودیا ہے۔ان کی کمی کوہمیشہ ہی محسوس کیاجائے گا۔ہندوستان کی سیاست میں___ایسا ہماراایقین ہے۔ [دسمبر ۱۹۹۰ء]
Islam is a divine religion. It is based on divine revelation and Sunnah of the Holy prophet (SAW). Tolerance, patience, forbearing and broadmindedness are the hallmarks of the last divine religion, Islam. In the article the ideology of co-existence has been discussed. Verses of the Holy book indicate importance of the tolerance. Similarly some events out of the Seerah of the Holy prophet (SAW) have been mentioned as references. In these pages it is impossible to explain scope of the subject; however efforts have been made to elaborate the Islamic perspective of tolerance. May Allah Almighty enable all of us to follow teachings of Islam in true spirit.
Laccases are oxidoreductases known as blue copper proteins as they contain copper atoms in their core structure.They catalyze oxidation of a variety of diphenols, polyphenols, amines, and polyaromatic hydrocarbons and simultaneously reduce molecular oxygen to water. These blue copper oxidases are widely disseminated among fungi, bacteria, insects and higher plants. The use of these ‘ecofriendly’ enzymes spans from paper and pulp industry to food applications and from textile industries to bioremediation processes as they have broad substrate specificity. In this project laccase was produced by Pleurotus ostreatus IBL-02 utilizing lignocellulosic waste material, wheat straw under pre-optimized conditions in solid state fermentation (SSF). Maximum yield (519.44 U/mL) along with 201.33 U/mg of specific activity was monitored in crude extract. The enzyme was then purified (3.4-fold) through a series of processes like ammonium sulfate precipitation, dialysis, ion-exchange chromatography by DEAE-Cellulose and gel filtration by Sephadex G-100. 67 kDa single band was observed by SDS-PAGE. Purified laccase was modified by various immobilization techniques like adsorption, encapsulation, covalent bonding and self cross-linking. Soluble and immobilized laccases was characterized via kinetic studies to analyze the consequence of temperature, substrate concentrations and pH on their activities. The presence of laccase in immobilized beads was confirmed by the use of Energy Dispersive X-ray (EDX) analysis. Native and immobilized enzyme were also utilizsed in different industrial applications like degradation of synthetic dyes, bio-remediation of dye based textile effluents and bio-stoning of denim jeans. The immobilized laccase showed better thermo-stability, reusability and sustainability during different environmental and industrial applications. The modified laccase as demonstrated to be an efficient dye decolorizer.