پاکستان جیسے ملک میں پولیس کی سستی اور عدم تعاون کا مرکز عمومی طور پر حدودمقدمات ہوتے ہیں ۔ پھر ان مقدمات کا دعویٰ بہت تاخیر سے دائر ہوتا ہے ۔ پھر بہت زیادہ تاخیر سے ان مقدمات کے کیس عدالتوں میں لگتے ہیں اور بآلاخر ان مقدمات کے فیصلے بھی بہت سالوں بعد ہوتے ہیں ۔اس کی بنیادی وجہ پولیس کی سستی ، رشوت کی عادات ، وکلاء کا عدم تعاون اور ججز کی تعداد کا کم ہونا ہے۔ پاکستانی معاشرہ اس وقت جرائم کا گڑھ بن چکا ہے ۔ روزاانہ کی بنیاد پر کافی زیادہ جرائم رپورٹ بھی ہوتے ہیں اور ان کی FIR بھی درج ہو تی ہیں ۔پھر یہ مقدمات عدلیہ کے سامنے پیش ہو تے ہیں ۔ اس طرح حدود وقصاص کے مقدمات سالوں تک چلتے ہیں۔ اس کے علاوہ اداروں کے آپس میں مربوط نہ ہونے کی وجہ سے حدود وقصاص کے مقدمات کو غلط طریقے سے نمٹایا جا تا ہے۔ وقت پر انصاف نہ ملنے کی وجہ سے مظلو م اور متضرر اپنے عدالتی نظام سے اور انصاف نہ ملنے پر ریاست سے بھی بد ظن ہو جاتے ہیں ۔
Family is a blessing from Allah Almighty. Family is the first institution of society which plays pivotal role in the moral, ethical and social development of an individual. But our contemporary family system has confronted with a number of religious social, and normal problems. Such pruners( چھانٹنا کانٹے( have enharossed our society from all caused and diffusing the moral and ethical values of society. It resulted in digestion of our family system. The degrade of entropy and chose is day by day in our society. However Islam outlays complete code of family life. It understands that it is the building foundation of every society so a clear guide as to how family structure should be built is outlined in detail in Islam. It is Provide a sample to solve all kinds of problems in the light of life of Hazrat Muhammad
Sugarcane is one of the major cash crops in Pakistan which has a prime contribution in the GDP. Average yield is very low due to pathogenic epidemics particularly sugarcane mosaic virus. Molecular biology tools have been adopted to increase the defense mechanism of sugarcane against viral attacks. Reverse transcription polymerase chain reaction (RT-PCR) was developed for the detection of sugarcane mosaic virus (SCMV) from the sugarcane samples collected from all major sugarcane cultivation districts in the Punjab, Pakistan. The segments of SCMV-CP gene were amplified from SCMV infected plants and sequenced. The sequencing results did not show 100% homology with any already reported isolate in the GeneBank data base. The sequences were submitted to the NCBI GeneBank and got the accession number KC200152 and KC249907 to KC249917. VIGS is a powerful tool used in functional genomics. Mechanism of VIGS is referred as post transcriptional gene silencing (PTGS) which involves the introduction of exogenous gene into the host plant as a result small interfering RNAs (siRNA) are produced which make a RISC assembly and the DICER like proteins chop the invading RNA as a defense mechanism. All plants naturally produce siRNAs as a defense against viruses and exogenous genes. If they are triggered as exogenous genes, the endogenous genes are silenced. In the present study, VIGS vector pBINTRA6 derived from TRV was modified by inserting SCMV-CP gene (amplified from infected sugarcane plants), GFP gene (amplified from Nicotiana benthamiana 16c GFP transgenic plant) and ChlI gene (amplified from Nicotiana benthamiana plant) for expression in N. benthamiana and sugarcane. Two SCMV-CP gene segments, 313 and 454 bp were cloned in pBINTRA6 to produce two VIGS constructs SCMV-CP/pBINTRA6-313 and SCMV-CP/pBINTRA6-454. Transient expression of GFP and ChlI genes silencing was studies in N. benthamiana GFP transgenic line 16c. SCMV-CP/pBINTRA6-313 has resulted relatively better silencing of GFP and ChlI genes in N. benthamiana than SCMV-CP/pBINTRA6-454. Two sugarcane cultivars HSF-242 and HSF-245 were selected for VIGS vector transformation of SCMV-CP/pBINTRA6-313 construct based on the results and observations of relatively more silencing of GFP and ChlI genes in N. benthamiana. The transformed (transgenic) plants were confirmed by PCR amplification of GUS reporter gene. Moreover, GUS assay was performed to confirm the transformation of plasmids as integral part of plant genome. Knockdown expression was studied by amplification of SCMV-CP gene by real time PCR. No amplification of SCMV-CP gene was seen in the transgenic sugarcane plants which confirmed the virus induced gene silencing of SCMV in sugarcane. Among the two sugarcane cultivars, HSF-242 demonstrated a strong expression for GUS assay. Bioassay i.e. transfection with virulent aphids (Rhopalosiphum maidis) was done on 4-6 fully developed leaves of transgenic sugarcane plants after shifting and acclimatization in soil pots under greenhouse conditions followed by molecular analyses. Results of bioassay determined that transgenic plants exhibited slightly slow growth and weak chlorotic phenotype when compared with the non-transgenic control plants. Results clearly demonstrated that transgenic plants showed resistance developed against sugarcane mosaic virus as a result of initiation of VIGS mechanism. The developed transgenic plants may be subjected to the biosafety studies and variety development in future studies. Moreover, VIGS optimized in the current study can be helpful for identification of new traits and control of other pathogenic diseases through PTGS which will play a key role in value addition and economy of the country.