Present study compared Esprit de corps and Culture of Public and Private Universities in Pakistan. The objectives of the study were to identify the strands of Esprit de corps and culture in Public and Private Universities, to compare the Esprit de corps and culture of Public and Private Universities and to analyze the relationship between Esprit de corps and culture in Public and Private Universities. The study was descriptive in nature.Population of the study comprised of faculty members of public and private universities. Two sets of questionnaires were used as tools for data collection from the Faculty members. Sample size of the study was 533. Data was analyzed by using percentage, mean, t test and Pearson Coefficient. The findings of the study concluded that the strands of Esprit de corps are more prominent in Private sector universities than Public sector universities and that there is a positive relationship between Esprit de corps and Culture of Public and Private Universities. It was also found out that there is a tangible difference between Esprit de corps and Culture of Public and Private Universities. It is recommended that in order to improve Esprit de corps in Public sector universities, there is a need to involve Faculty members in decision making process and Faculty members must be provided with feedback about their work. Skill development training programs must be arranged at regular intervals to polish the leadership skills, communication skills, team making skills, collaborative skills and other life skills to improve the weak areas of educational managers.