Search or add a thesis

Advanced Search (Beta)
Home > Assessment of Anti-Proliferative and Anti-Inflammatory Potential of Thymoqunione Tq and Thymohydroquinone Thq Coupled With Poly Lactide-Co-Glycolide Nanoparticles

Assessment of Anti-Proliferative and Anti-Inflammatory Potential of Thymoqunione Tq and Thymohydroquinone Thq Coupled With Poly Lactide-Co-Glycolide Nanoparticles

Thesis Info

Access Option

External Link

Author

Butt, Ayesha Siddique

Program

PhD

Institute

Lahore College for Women University

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Environmental Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12007/1/Ayesha%20Butt--Environmental%20Science%202019%20lcwu%20lhr%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676724521334

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Current study was designed to extract phytochemical compounds, thymoquinone (TQ) and thymohydroquinone (THQ) from indigenous herbs i.e., Nigella sativa (seeds) and Thymus vulgaris (aerial parts). Extracted compounds were further subjected to evaluate their anti-proliferative and anti-inflammatory activities and loaded in novel biodegradable drug carrier system including pectin and poly lactide-co-glycolide (PLGA) to achieve a controlled drug release system. Drug carrier system was synthesized by extracting pectin from waste peels of citrus fruits (lemon, orange and grape fruit). To enhance the stability, FDA approved PLGA drug carrier was physically cross-linked with pectin hydrogel. This may potentially introduce an opportunity to utilize organic waste in pharmacology.Results showed that soxhlet extraction at ratio of n-hexane: methanol (1:4) has been the appropriate combination to extract maximum yield of TQ from N. sativa (56.13%) and T. vulgaris (22%). THQ extraction was not achievable due to its very less stability. HPLC quantification of essentials oil of N. sativa showed higher amount of TQ i.e., 368.55±0.12 mg/kg (Mean±SEM) and T. vulgaris had 197.58±0.7 mg/kg. The pectin was successfully extracted in maximum yield from waste peels of lemon as compared to other two samples i.e., 35.3±0.66 g (Mean±SEM). Characterization of pectin extracted from each samples showed orange pectin potentially more suitable for hydrogel formation. SEM analysis confirmed the nanoparticles of TQ efficaciously produced loaded in PLGA-PEG at average size of 280 nm. TQ has been proved to be potent anti-proliferative and anti-inflammatory agent by in-vivo antioxidant assays (DPPH and FRAP assay), MTT assay against HeLa cancer cell line and SRB assay against HCT116 colon cancer cell line. Inhibition concentration (IC50) of TQ against HeLa and HCT116 cancer cell lines showed less significant difference (p > 0.05) to the IC50 of control commercial anti-cancer drug (cisplatin). TQ and its nanoparticles loaded in pectin hydrogel showed delayed release of TQ thus enabling the controlled drug release from 24 to 72 hours. TQ thus obtained can be utilized against early treatments of variety of cancers as can be observed from IC50 obtained against HeLa and HCT116 cancer cell lines. Findings of the current study indicate the potential of local resources in effectiveness against cancer. Therefore, increased incidences of cancer can be reduced among impoverished areas.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

مہر آپ جدوں مہربان کردا

مہر آپ جدوں مہربان کردا

رب مشکلاں سب آسان کردا
شاہ رگ دے کولوں وی ہے نیڑے

آپ وچ قرآن فرمان کردا
ہک وار دیدار نصیب ہوندا

جان لکھ کروڑ قربان کردا
میرا ہیں تے بن کے رہویں میرا

ایسے گل اُتّے بندہ مان کردا
دکھی دردی نوں لیندا لا سینے

اک وار چا یار احسان کردا
نِت پنڈ گناہاں دی کراں کٹّھی

نِت فضل ہے رب رحمان کردا
تیرے پیار اندر عمر گئی ساری

ہور سوچ کی ایہہ نادان کردا
عشق دسدا اپنا آپ جس دم

عقل سوچ دانائی حیران کردا

پاکستان کی سیاسی و قومی یکجہتی کے فروغ میں اُردو زبان کا کردار

Urdu is the national language of Pakistan and plays a significant role in promoting political and national solidarity in the country. Languageserves as a unifying force that brings together people from different linguistic and cultural backgrounds and fosters a sense of shared identity and belonging. One of the main ways in which Urdu promotes political and national solidarity is through its use as a medium of communication across the country. Urdu is widely spoken and understood in Pakistan, and its use as an official language ensures that people from all regions of the country can communicate effectively with each other. This helps to promote a sense of unity and understanding between different groups, which is essential for building a strong and cohesivenation. In addition to its role as a medium of communication, Urdu also has a rich literary and cultural heritage that is deeply intertwined with the history and identity of Pakistan. Urdu poetry, literature, music, and art have played a significant role in shaping the country's cultural landscape and providing a shared cultural identity for its people. Furthermore, usingUrdu in political discourse and media plays a crucial role in promoting national unity and solidarity. Political leaders and media outlets use Urdu to communicate with the public. Thelanguage is often used to express nationalistic sentiments and promote a sense of pride in Pakistan's culture and heritage. Urdu plays a vital role in promoting Pakistan's political and national solidarity byserving as a unifying force that brings together people from different regions and cultural backgrounds. Its use as a medium of communication, its rich literary and cultural heritage, and its role in political discourse and media all contribute to fostering a sense of shared identity and belonging among Pakistanis.

Socio-Economic Impact of Drought on Pakistan= a Climatological Forecasting Analysis

Pakistan, located between 23o 42´ to 37o 06´ N and 60o 54´ to 77o 52´ E is girdled by the dominating Himalayan ranges in the north under the influence of calms of cancer and shifting of doldrum, with greater part of the country experiencing arid climate alongwith recurring drought. The spatio-temporal variability in atmospheric water and thermal regime is reflected in the high variability of precipitation and resultant extended droughts and floods. Consecutive failure of precipitation leads to meteorological drought; reduced stream flow leads to hydrological drought, in turn resulting in agricultural drought which disturbs the agrarian economy. Pakistan being an agricultural third world country, its economic and hence social milieu is inherently influenced by its climatic variations and severities. Agriculture being the lynchpin of its economy, Pakistan’s GDP has declined from 50 to 22% from 1949-50 to 2009-10. The drought of 1998- 2002 was reported as the worst in the last 50 years. The study is based on the hypothesis that drought, among other natural disasters is the worst enemy of the economy of Pakistan and hence on its social set up. Searching a way out of this critical environmental condition is necessary for ameliorating the socio-economic status of this third-world country. Furthermore, all types of drought originate from meteorological drought in Pakistan. The objectives of the research include revelation of meteorological/climatological nature of drought, classification of Pakistan’s climate, alongwith demarcation of climatologically affected sectors. Determination of the effects of drought and desertification, identification of the relationship between teleconnection phenomena and annual precipitation are also included in the objectives. Determination of the effect of drought on river flow is related to the assessment of socio-economic impact of hydrological drought on the country, as agriculture is inherently related to water availability in the perspective of agricultural drought leading to socio-economic drought. Formulating methods of assessment of meteorological drought is essential from the prediction point of view, which is an essential part of the thesis. The study area encompasses the macro-area of Pakistan served by 51 meteorological observatories. Case study of three selected villages, representative of arid and semi-arid conditions from three most drought prone provinces has been made with the help of questionnaire surveys to collect first hand information of socio-economic impact of drought. Methodology of the study includes in its purview, processing of data collected both from primary as well as secondary sources. Assorted statistical softwares (viz. XLSTAT, MYSTAT, MiniTab, etc.), methods and self made routines have been applied in the temporal study and forecasting module. Some GIS (software) e.g., arcGIS, Surfer, etc. have been employed for spatial study, missing climate data mining, presentation as well as climatic forecasting analysis. Graphical method for missing climate data mining is one of the original contributions of this thesis, alongwith Standardized Rainfall Evapotranspiration Index (SREI), which has been proposed for drought monitoring. The study has revealed that deficit in precipitation and insufficient water resources are the major cause of vulnerability to drought. Hydrological, agricultural and socio-economic droughts are rooted in meteorological drought. Increasing trends of mean temperatures and high potential evapotranspiration dominates throughout the country; which is a sign of climate change. The global source and sink teleconnection phenomena, La-Nina plays a vital role in maneuvering precipitation regions in Pakistan. Droughts severely impact the agricultural productivity and subsequently the rural social status. In order to aid in climatological forecasting a modified formula has been proposed, as an original contribution in the thesis, for calculating sunshine hours from total cloud amount, for the calculation of FAO PenmanMonteith Evapotranspiration. Suggestions for improving socio-economic conditions with reference to drought hazards include reductions in population growth rate and enhancement of agro-based business in order to improve social status of 80% of its population which is directly or indirectly related to agriculture. Development of new and enhancement of old water reservoirs is essential. Construction of small dams in the northern areas and barrages in the southern region cannot only help in reduction of floods but storage of water for use during droughts. Plantation of vegetation, capable of reducing salinity and enhancing water table conditions in the coastal areas e.g. coconut trees, may not only enhance the economy of the area but also improve soil conditions leading to enhanced agricultural productivity. Last but not least, a forecasting equation has been developed for assessing the country’s GDP growth rate based on climate and agricultural data.