13مئی یوم عزم
جس دن صحافیوں کو کوڑے مارے گئے ۔13مئی 1978 ء کی شام لاہور کے تین نوجوان صحافیوں کو فوجی حکومت کے حکم پر برہنہ کر کے پندرہ کوڑے مارے گئے تو انہوں نے اپنے دانت بھینچ لیے تھے اور درد کی آواز دبا لی تھی ۔انہوں نے فوجی دہشت سے خوفزدہ ہو نے سے انکار کر دیا تھا ۔ شام آٹھ بجے لاہور کوٹ لکھپت جیل کے میدان میں تین برہنہ آدمیوں کو لا کر ان کے ہاتھ پائوں ایک ٹکٹکی کے تختے سے باندھ دیے گئے ۔ایک پہلوان نما آدمی چمڑے کے کوڑے کو گھماتادور سے بھاگتا ہوا ان آدمیوں کے قریب آ تا اور ان کے اجسام پر پانچ پانچ بار اپنا کوڑا برساتا ۔ٹکٹکی کے سامنے کچھ دور تقریباََسو باوردی فوجی اہلکار اور سول سرکاری اہلکار کرسیوں پر بیٹھے دنیا کے اس منفرد واقعہ کا تماشا دیکھ رہے تھے ۔جس میں پاکستان کے تین صحافیوں کو پریس کے خلاف فوجی اقدامات پر احتجاج کر نے کے جرم میں کوڑوںکی سزا دی گئی ۔جن تین صحافیوں کے جسم پر ضیاء الحق کے کوڑے پڑے ان میں ایک چوبیس سالہ نوجوان اور روزنامہ کے سب ایڈیٹر خاور نعیم ہاشمی تھے ۔وہ اپنے اس دور کی یادوں کو کچھ یوں بیان کرتے ہیں ۔’’یہ جنرل ضیاء الحق کے مارشل لاء کا ابتدائی زمانہ تھا جنہوں نے 5جولائی 1977ء کو وزیر اعظم ذوالفقار علی بھٹو کومعزول کر کے اقتدار سنبھالا تھا ۔بیس سے زیادہ اخبارات اور رسائل مارشل لاء حکومت کی پالیسیوں کی مخالفت کر نے کی وجہ سے بند کر دیے گئے تھے اور تمام اخبارات پر سخت سنسر شپ عائد تھی ۔ان میں پیپلز پارٹی کا ترجمان اخبار روزنامہ مساوات بھی شامل تھا ۔خاور نعیم ہاشمی مساوات اخبار میں کام کرتے تھے ۔ان کا کہنا ہے کہ پریس پر...
Pakistan’s involvement in the US war on terrorism was a tragic decision. No option was left for the ruling elite of Pakistan except to join the global war on terrorism and to take a U-turn from the support of Taliban’s regime in Afghanistan which was duly recognized by Pakistan’s government in 1996. It was expected by the policy-makers of the US that the alliance with Pakistan would provide extraordinary strength in combating the Al-Qaeda and other affiliated conglomerates in Afghanistan as well as in borderland area. However, after fifteen years of war, the alliance has enfeebled despite their mutual understanding regarding the objectives envisaged in the Strategic Partnership. The war on terrorism has now been escalated from Afghanistan to Pakistan and it has provided space to religious extremism, militancy, intolerance, ethnic division and sectarianism. There is no denial to the fact that religious extremism and terrorism are common threat and have damaged both the countries yet Pakistan has sacrificed more than the US in terms of human and material loss. Nevertheless, blame game and trust deficit is on the rise from both sides. This article focuses first on the joint ventures that the US and Pakistan mutually initiated to curb militant bloodbath in Afghanistan as well as in the border region. Secondly, it will explore factors responsible for increasing trust deficit between the partners. The study will not only provide deep understanding about the prevailing issues between Pakistan and the US but will also give true pictures to streamline the methodology for negotiating with each other in future.
β thalassemia is the most prevalent autosomal recessive disorder characterized by absence or reduced production of hemoglobin (Hb) levels, primarily caused by mutations on β globin locus. β thalassemia is heterogeneous at the molecular level, presenting variable phenotypes accompanied with severe medical complications. Current standard of care for clinical management of β thalassemia includes regular, long-life safe blood transfusion along with appropriate iron chelation therapy. At present, the only permanent cure is bone marrow transplantation. An emerging and exciting therapeutic approach to handle β thalassaemia is production of fetal hemoglobin (HbF) which is major Hb of fetal life. In recent years, Hydroxyurea (HU) has proven to be a promising HbF augmenting agent but response to HU therapy varies from transfusion elimination to insignificant clinical response. Various approaches are being made to understand the mechanism HbF augmentation with differential responses. Advancement in proteomics offers an efficient tool to study differential proteome in response to treatment leading towards precision and personalized medicine. This study is designed to improve mechanistic understanding of proteomic changes that HU therapy exerted on β thalassemia patients, in consort with deciphering differential protein expression in HU responder and non-responder. Firstly, samples were subjected to twodimensional gel electrophoresis to assess differentially expressed proteins. Later, differential proteins were identified by label free quantitative proteomics approach. Two hundred and eighty seven proteins were identified with two or more unique peptides in samples studied. Among these, twenty eight proteins were found to be significantly different in pre versus post HU treated β thalassemia patients at probability of < 0.05. Eighteen proteins were down-regulated while ten were found to be up-regulated after HU treatment. Clinically important proteins include Hemopexin (HPX), Haptoglobin (HP), Haptoglobin-related protein (HPR), Hemoglobin subunit beta (HBB), Hemoglobin subunit delta (HBD), Hemoglobin subunit alpha (HBA1), Protein S100-A8 (S100A8), Apolipoprotein L1 (APOL1), Apolipoprotein C-I (APOC1), Transferrin receptor protein (TFRC), Complement C4-A (C4A), Apolipoprotein A (LPA), Ceruloplasmin (CP) and Ficolin-3 (FCN3). HU therapy in β thalassemia patients started reverting protein profile towards healthy pattern, in addition with decrease in transfusion requirements. A follow up study on plasma of HU treated β thalassemia patients was performed to compare proteomic profile of HU responder and non-responder. Twenty six proteins were found to be differentially expressed in HU responder versus non-responder at p < 0.05. Among these, fifteen proteins showed a significantly increased level while eleven proteins revealed a decreased in expression. Clinically relevant altered proteins in HU responder are Peroxiredoxin-2 (PRDX2), Carbonic anhydrase 1(CA1), Hemoglobin subunit gamma-1 (HBG1),Hemoglobin subunit beta (HBB), Hemoglobin subunit delta (HBD), Hemoglobin subunit alpha (HBA1), Properdin (CFP), Cholinesterase (BCHE), Phospholipid transfer protein (PLTP) and Plasma protease C1 inhibitor (SERPING1). We suggest that further research would be required for validation of identified proteins in large cohort to endorse as potential predictive biomarker for HU therapy. Considering the association of oxidative stress with β thalassemia, we also studied markers of oxidative stress in response to HU therapy in β thalassemia covering Paraoxonase1 (PON1), Reactive oxygen species (ROS), and Malondialdehyde (MDA). Although PON1 serve as an antioxidant to reduce the adverse effects of the oxidative stress in β thalassemia, our results indicate that mode of action of HU may not directly be through oxidative imbalance