تفسیر دعوۃ القرآن کے امتیازات و خصائص

Quran is the word of Allah Almighty, therefore, in order to understand it in the true sense, a person has to sharpen his or her intellectual ability as well as increase the knowledge. When one is at a particular intellectual level, only then can he or she start understanding the true message, which Allah Almighty conveyed through words of Quran. Pertaining to the explanation of Quran for the understanding of general audience, different people have tried to write the Tafseer of Quran. Although Muslims recite Quran and try reading it with translation, however, the reading of Tafseer has its own importance. “Tafseer Dawat-Ul-Quran” is written by Abu Nauman Saifullah Khalid and published from Dar-Ul_Undalus Lahore in 2010. This Tafseer has been written in the light of the Quran, Hadith, and the sayings of the companions of Prophet. In this Tafseer correct tradit-ions has been included and unauthentic traditions has been avoided. In  theological  interpretations,   the  words  of  Qur'an are  explained  in  the context of the Qur'an itself or the sayings of The  Holy Prophet(ﷺ). This type of interpretation is called “Tafseer Bilmasur”.“Tafseer Dawat-Ul-Quran”is representative of Tafseer Bilmasur. In this research article, the Salient features of “Tafseer Dawat-Ul-Quran” are discussed.

Mutation Spectrum in Pakistani Patients With Inherited Metabolic Disorders

Inherited metabolic disorders constitute a diverse class of genetic diseases caused by impairment in biochemical processes because of defective enzymes or transporters consequent upon which compromised conversion of substrate into the product takes place. These disorders are manifested phenotypically with a wide overlapping spectrum of signs and symptoms that could either be due to accumulation of toxic upstream substrates, insufficient production of downstream products or abnormal alternative substrate metabolism. Biochemical and molecular investigations help in diagnosis of hereditary metabolic disorders and that if attempted early, could improve therapeutic outcome in some cases. The current study was performed to investigate the spectrum of genetic variants in Pakistani patients with inherited metabolic disorders. A cohort of sixty-eight patients was enrolled in the study from local hospitals. Twenty-five of these patients were diagnosed with inherited unconjugated hyperbilirubinemias, one with GM1 gangliosidosis, nineteen with various types of mucopolysaccharidoses (MPS), and twenty-three with Wilson’s disease. Genomic DNA samples of these patients were subjected to Sanger sequencing of respective genes. The identified variants were confirmed in families of the patients for segregation. Sequence analysis of UGT1A1 in twenty-five patients diagnosed with inherited unconjugated hyperbilirubinemias identified 16 different variants, six of which were novel. The c.622-625dupCAGC and c.1021C>T were the most frequently observed UGT1A1 variants. DNA sequencing of GLB1 in one patient diagnosed with GM1 gangliosidosis identified a novel homozygous 2-bp deletion c.881-882delAT (p.Tyr294Terfs) in exon 8. Among nineteen patients diagnosed with various types of mucopolysaccharidoses during the study period, MPS type I was the most prevalent phenotype. DNA sequencing of IDUA in MPS type I patients revealed a diverse spectrum of variants spanning the whole gene. A missense variant c.1469T>C (p.Leu490Pro) was the most common variant identified in five patients in homozygous condition. Mutations identified in other MPS types were all private mutations and include c.1006+1G>C, c.1165C>T in IDS, c.531+5G>A in NAGLU, c.902G>A, c.1175C>T, c.IVS4-1G>A in GALNS and c.511-512delGG in ARSB. DNA sequencing in a cohort of twenty-three Wilson’s disease patients identified a diverse spectrum of 18 ATP7B variants spanning over the whole gene. The mutation detection rate was 71.7% and seven of the identified variants were novel. The most frequently observed ATP7B variant was c.3809A>G followed by c.3182G>A. To our knowledge, this is the first comprehensive study of inherited metabolic disorders from Pakistan. It should be helpful in neonatal screening, improved genetic counseling, and prenatal diagnosis in the affected families.