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Home > Determination of Benzo A Pyrene Bap in Biological Samples and Detection of Genetic Damgae in Subjects Working at Petrol Pumps

Determination of Benzo A Pyrene Bap in Biological Samples and Detection of Genetic Damgae in Subjects Working at Petrol Pumps

Thesis Info

Access Option

External Link

Author

Butt, Faiza Siddique

Program

PhD

Institute

Lahore College for Women University

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Environmental Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12115/1/Faiza%20siddique%20Butt-2019%20envir%20sci%20lcwu%20lhr%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676724601633

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The present study deals with the determination of benzo(a)pyrene in biological samples and detection of genetic damage in the subjects working at petrol pumps. The multidisciplinary study involved questionnaire survey and analysis of biological samples. The questionnaire survey was carried out in 107 petrol pumps of Lahore and Gujrat districts for the assessment of health status of the workers (n=1000) and risk factors in their workplace settings. The biochemical tests included; detection of benzo(a)pyrene in blood and hair samples by HPLC (n=200). Buccal micronucleus cytome assay (BMCyt) was carried out for the identification of nuclear anomalies i.e. micronucleus (MN), binucleus (BN) for the evaluation of genotoxic parameters and cytotoxic damage by karyorrhexis (KR) and karyolysis (KL) cells. The DNA damage was further assessed by comet assay through total comet scoring (TCS). Genetic polymorphism was detected in associated genes in selected blood samples of the petrol pump workers through polymerase chain reaction (PCR). Data was analyzed through ANOVA, t-test (p<0.05) and Pearson correlation. Questionnaire survey revealed the study respondents had low socioeconomic and educational status, poor dietary habits, various health problems and specially no use of PPE during work. Analysis of samples showed significantly higher concentration of BaP in blood (µg/l) and hair (µg/g), frequencies of buccal cell anomalies and TCS in the petrol pump workers (exposed) as compared to non-exposed subjects. The PCR of the selected samples evidently revealed polymorphism in the associated genes (CYP1A1 and GSTM1) hence pointing towards the more probability of susceptibility to diseases related to lungs. The results were compared on the basis of age, job duration, smoking habits, working hours per day and job types. The subjects of higher ages, long job duration, smokers and longer working hours per day were found with significantly higher BaP concentration in blood and hair. Similarly the buccal cell anomalies and total comet scores was also significantly higher in these subjects. The BaP concentration in blood was strongly corelated to the MN frequencies in buccal cells and TCS in blood cells of the petrol pump workers. The results are depictive of the fact that low socioeconomic status, less education, lack of awareness, no use of PPE, personal hygiene and smoking habits appear to be the contributory factors. Results of BaP in blood and hair revealed that the petrol pump workers are likely to get occupational exposure to PAHs like BaP and other such substances in their workplace because of many reasons especially due to ignorance, nonuse of PPE, in effective regulations, monitoring as well as the faulty working practices. The results of the study are significant and provide enough evidence with reference to the health and safety issues at the petrol pumps in Pakistan. On the basis of the results; recommendations are drawn for the future work with reference to health risk assessment and management at work. It also advocates for the formulation of specific rules for petrol pump workers.
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اطہرؔ صدیقی

اطہرؔ صدیقی(۱۹۳۵ء۔پ) کا اصل نام محمدیسیٰن صدیقی اور اطہرؔ تخلص کرتے تھے۔ آپ چوہان حال برہان پور تحصیل پسرور میں پیدا ہوئے۔ آپ معروف شاعر پروفیسر حفیظ صدیقی کے بھائی تھے۔ حفیظ صدیقی کی راہنمائی میں اطہر نے زمانہ طالب علمی میں شاعری کا آغاز کیا تو ان کا کلام ملک کے معروف ادبی رسائل و جرائد میں شائع ہونے لگا۔(۹۱۶)

اطہرؔ کا پہلا شعری مجموعہ’’ کاکل غم‘‘ غزلوں اور نظموں پر مشتمل ہے جو۱۹۸۷ء میں شائع ہوا۔دوسرامجموعہ کلام’’ذوق سفر‘‘ کے نام سے ۱۹۸۹ء میں صدیقی پبلی کیشنز لاہور سے طبع ہوا۔ یہ مجموعہ غزلیات پر مشتمل ہے۔ تیسرا شعری مجموعہ’’آبرؤے غم‘‘۱۹۹۰ء میں صدیقی پبلی کیشنز لاہور سے شائع ہوا۔ یہ مجموعہ بھی غزلیات پر مشتمل ہے۔ چوتھا شعری مجموعہ ’’گردِ مسافت‘‘غزلیات اور نظموں پر مشتمل ہے۔

اطہر ؔصدیقی کی شاعری کا بڑا موضوع عظمت انسان ہے ۔وہ اپنی شاعری میں حضور ؐ کی ذات اقدس کے شیدائی نظر آتے ہیں۔ ان کے نزدیک حضرت محمدؐ کی ہستی عظمت انسان کی علامت ہے۔ اور وہی مثالیِانسان کا نمونہ ہیں۔انھوں نے نعت میں ہی نہیں بلکہ اپنی نظم اور غزل میں بھی عظمتِ انسان کی حقیقت کا اظہار کیا ہے:

ذرے ذرے سے پوچھ دیکھا ہے

 

-دشت در دشت کون رہتا ہے

 

5کون رہتا ہے لا مکاں میں اب

 

3کس کی رعنائیوں کا چرچا ہے

(۹۱۷)

ƒاطہرؔ کی شاعری عزم و ہمت ،جوش ،جواں جذبوں ،جستجو اور بلند حوصلوں سے بھر پور شاعری ہے ۔وہ اپنی شاعری میں کہیں بھی پست ہمت نظر نہیں آتے۔ان کے ہاں جوش اور جذبات کی شدت قاری کے حوصلے کو بلند...

المحكم والمتشابه وموقف المفسر منهما

The Mohkam and Mutashabeh is a renowned terminology of the Quranic Sciences and commentators of the Holy Quran described it in details, according to root words of Mohkam, it means Stopping and perfecting the things, this basic meaning can be seen in all the types and variations of this word. On the other hand we have the word Mutashabeh which root meaning is complication and unclearness. If we discuss both of the words as a terminology of the Quranic sciences, we can define Mohkam as “one which define itself without any other thing” or “one which has no need to be defined by something else” and Mutashabeh is “one which can’t define itself and need to be explained by someone else”. We will move on to discuss both terms in Holy Quran as a terminology to describe its multiple variations in the Holy Quran, its types and further we will discuss that why the Holy Quran contains both terms, in other words, we can say which are the logics and reasons of including Mutashabeh verses in the Holy Quran. In addition, we will mention the point of views of various renowned commentators and fields experts which give us a clear and sound concept about both of the terms.

Improvement of Yield Attributes, Oil and Protein Contents in Sunflower Helianthus Annuus L. Through Biometrical Genetic Analysis

Sunflower is a major oil seed crop in which genetic variance could be exploited considerably for higher seed yield, oil contents and protein percentage by evolving superior F1 hybrids.The best F1 hybrids however can be obtained by crossing inbred parents with good general and specific combining ability due to higher additive, dominance and complementary epistatic genes. Foremost improvement in sunflower breeding requires the urgency in developing the heterotic hybrids which may achieved by exploiting combining ability and hybrid vigour in F1 hybrids developed from crossing genetically diverse female lines with male restorers. The sunflower F1 hybrids are considered more stable, uniform in plant stand, highly self-fertile, extremely productive in terms of seed yields, oil content, earlier in maturity, resistant to lodging, diseases and insect-pests. The present studies were carried-out at experimental field of Oil Seeds Section Agriculture Research Institute, Tandojam from 2009 to 2011. Six cytoplasmic male sterile female lines viz. ARG-0306,64-A-93, PSF-025, SF-187, ARG-0405 and ARG-0505 and three testers RHP-46, RHP-64 and ARG-0106were crossed in a line x tester mating design, thus 18 cross combinations were developed for evaluation and genetic analysis. The characters studied were; days to 50% flowering, days to 90% maturity, plant height, stem girth, head diameter, number of achenes plant-1, 1000-achene weight, seed yield plant-1, seed yield in kg ha-1, oil content and protein percentage. The analysis of variance revealed significant differences among the parents, F1 hybrids and parents vs. hybrids for phenological, seed yield traits, oil content and protein percentage in F1 and F2 hybrids. The significance of mean squares due to parents vs. hybrids recorded for all the traits studied suggested the scope of heterosis breeding in sunflower, whilst the significant differences for lines x tester interactions indicated the importance of specific combining ability (SCA) of hybrids which suggested the involvement of dominant genes in the expression of studied traits. The significant mean squares due to lines and testers both determine the general combining ability (GCA) revealed the predominance effect of additive genes advocating the traits. Significant genetic variability among the plant traits is particularly valuable because variations in plant traits would allow further improvement in sunflower seed yield and quality traits. The major role of dominant genes was very apparent because the ratio of σ2SCA:σ2GCA was significantly higher and degree of dominance (σ2D/ σ2A)1/2 was also greater than unity. These results indicated the preponderance of dominant gene action suggesting the feasibility of hybrid crop development. General combining ability estimates indicated that CMS lines SF-187, 64-A-93 and ARG-0405 whereasfrom testers RHP-46 were found as the best general combiners for early maturity, seed yield, oil and protein traits, hence these parents may be chosen for hybridization programmes to evolve profitable hybrids or synthetic varieties. Specific combining ability and heterotic effects are very important estimates for determining the suitability of F1 hybrid crop development. The hybrids such as SF-187 x RHP-46, 64-A-93 x RHP-46, PAC-ARG-0405 x PAC-ARG-0106, 64-A-93 x RHP-46 and PSF-025 x RHP-64 which involved parents with high x high and high x low GCA effects, exhibited higher positive SCA and heterotic effects for seed yield, oil content and protein percentage while they expressed negative yet desirable SCA effects for phenological traits and plant height. These results suggested that such hybrids are advantageous for the exploitation of hybrid crop development or selection of transgressive segregants in earlier filial generations. The narrow sense heritability estimates were generally low suggesting greater portion of dominant variances against the additive. Heritability results thus suggested that selection for studied traits may be exercised in later segregating generations while giving the opportunity of recombination to occur between desirable genes. Correlation results generally suggested that head diameter and 1000-achene weight have shown significant positive associations withall seed, oiland protein traits, henceboth the traits may be used as most reliable selection criteria to improve yield, oil andprotein traits in sunflower breeding programmes.