Hepatocellular carcinoma (HCC) ranks as 2nd most common cause of cancer deaths globally. Hepatocellular carcinoma is primary malignancy of the Liver and occurs predominantly in patients with underline chronic Liver disease and cirrhosis. The incidence of HCC is highest in Asia and Africa, where the endemic high prevalence of hepatitis B and hepatitis C strongly pre-disposes to the development of chronic Liver disease and subsequent development of HCC. HCC is rising in Pakistan and represent the most common cancer in adult males. Hepatitis C is single largest cause of HCC in Pakistan. The absence of national cancer registry and screening program are spreading hepatitis C and HCC fastly in Pakistan. Lactoferrin is a glycoprotein and is an important minor protein fraction of milk and milk products. It has magical properties like antimicrobial strongly binds with iron, boost immunity as an anti cancer agent and potentiation of macrophage cytotoxicity. Lactoferrin can also activate NK cells and arrest cell proliferation and proliferation of epithelial tumor cells lines. The anti cancer activity of Lactoferrin was studied in this research to evaluate its protective effects by induction of Liver cancer in rats model. According to several studies immunity stimulation and enhancement of influence of Lactoferrin against carcinogenesis is highly determined. Absorption potential of Lactoferrin in animal model results in its increased distribution and biological impact.