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Home > Microbial Acc-Deaminase May Improve the Efficiency of Rhizobium Inoculation in Mung Bean under Salt Affected Conditions

Microbial Acc-Deaminase May Improve the Efficiency of Rhizobium Inoculation in Mung Bean under Salt Affected Conditions

Thesis Info

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Author

Maqshoof Ahmad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2011

Thesis Completion Status

Completed

Subject

Public Adminisration

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/1340

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676724865714

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High ethylene concentration under different environmental stresses such as salinity is one of the contributing factors for premature senescence of different plant parts. Plants under salinity stress produce increased levels of ethylene which inhibit the plant growth. Microbial 1-aminocyclopropane-1-carboxylic acid (ACC)-deaminase is the enzyme which is present in some strains of plant growth promoting rhizobacteria (PGPR). These PGPR alone and also in combination with rhizobial strains promote plant growth by lowering the endogenous level of ethylene along with some other mechanisms. A number of rhizobacterial and Rhizobium strains were isolated from rhizosphere and nodules of mung bean, respectively. The rhizobacterial strains were screened on the basis of ACC metabolism assay. The selected rhizobacterial strains containing ACC-deaminase activity and Rhizobium were assessed for their ability to tolerate salt stress by conducting osmoadaptation assay. These strains were further screened for their ability to promote growth of mung bean seedlings under salt-stressed axenic conditions in growth pouch/jar trials. Three most effective strains of PGPR (Mk1, Pseudomonas syrigae; Mk20, Pseudomonas fluorescens and Mk25, Pseudomonas fluorescens Biotype G), and Rhizobium phaseoli (M1, M6 and M9) were screened in co-inoculation for their growth promoting activity at original, 4 and 6 dS m-1 under axenic conditions. Strains from three most effective combinations were tested alone and in combinations in pot and field trials for their potential to improve growth, nodulation and yield of mung bean under salt- affected conditions. Results showed that salinity stress significantly reduced plant growth, some physiological parameters, nodulation and yield but inoculation with PGPR containing ACC-deaminase and rhizobia improved these, thus reducing the inhibitory effect of salinity. However, their combined application was more effective under salinity and the co-inoculation with PGPR strain Pseudomonas fluorescens and Rhizobium phaseoli was the most efficient for improving growth, nodulation, physiology and yield of mung bean. The effect of high ethylene concentrations on plant growth and performance of these strains for reducing this negative impact was also evaluated by conducting classical triple-response bioassay. Intensity of the classical triple response decreased due to inoculation with these strains as the root/shoot length of inoculated mung bean seedlings increased while stem diameter decreased which is typical indication of the dilution in classical triple response. Such bacteria could be very effective as co- inoculants to improve growth, physiology, nodulation and yield of mung bean under salt affected conditions. However, the degree to which these inoculants impart benefits to plant growth can vary with the conditions and PGPR strains. A PGPR strain with multiple traits could be more useful under diverse conditions compared to a strain containing single trait.
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شاہ اسد الرحمن قدسی

شاہ اسدالرحمن قدسیؒ
(محمود الرحمن )
بہت سے انسان موت کے بعد فراموش کردیئے جاتے ہیں، لیکن بعض اس مرتبے کے ہوتے ہیں کہ ان کی یاد لمحے بھر کو بھی دل سے محو نہیں ہوتی، اور زندگی کے ہر موڑ پر ان کی کمی شدت سے محسوس کی جاتی ہے۔
ایسی ہی عالی مرتبت ہستیوں میں حضرت شاہ اسد الرحمن قدسی علیہ الرحمۃ بھی تھے، طریقت ہوکہ شریعت، قلندری ہوکہ درویشی، علم ہوکہ عرفان، ادب ہوکہ آگہی، فلسفہ ہوکہ حکمت، تفکر ہوکہ تصوف، ہر صنفِ میں انھیں مقام اولیت حاصل تھا۔
برصغیر پاک و ہند کے تمام اکابر آپ کی فکر و نظر، علم و دانش، تبحرعلمی، قلندارانہ شان، حسن اخلاق، شفقت و محبت اور روحانی مقام و مرتبے کے معترف رہے ہیں، ہر مکتبۂ خیال کے لوگ آپ کی خدمت میں حاضر ہوکر سکون و اطمینان کی دولت سے مستفید ہوتے رہے ہیں، ہر فکر و تشویش ان کی صحبت میں کالعدم ہوجایا کرتی تھی، اور ہر عقدۂ لاینحل اس پیرکامل کی مجلس میں حل ہوجایا کرتا تھا اور سائل آستانہ قدسی سے شاد کام وبامراد لوٹتا تھا۔
آپ کا اسم گرامی ناصر الدین محمد اسدالرحمن قدسی تھا۔ آپ کے جدامجد شیخ الاسلام شاہ ابوالمکارم، جن کا سلسلۂ نسب حضرت امام جعفرؓ صادق سے ملتا ہے، سبزوار کے رہنے والے تھے، وہاں سے ہجرت کرکے ہندوستان تشریف لائے اور قطب الاقطاب حضرت شیخ عبدالقدوس گنگوہیؒ کے خاندان میں متاہل ہوئے، آگے چل کر اس خاندان میں ایک بہت مشہور بزرگ حضرت شاہ نجف علی گزرے ہیں، جو حضرت رفیع الدین محدث دہلویؒ کے شاگرد و خلیفہ تھے، موصوف کی اہلیہ محترمہ حضرت مولانا رشید احمد گنگوہیؒ کی حقیق بہن تھیں، جن سے حضرت شاہ حبیب الرحمن پیدا ہوئے۔
آپ کی والدہ محترمہ علامہ محمد اسحق کی دختر تھیں۔ ان کی زبان فارسی تھی، اردو...

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Subscription or Subversion: Gender Representation in Pakistani Television Commercials

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