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Productive and Reproductive Performance of Indegenious and Cross Bred Dairy Calttle in Muzaffarabad Azad Jammu and Kashmir

Thesis Info

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Author

Khan, Muhammad Ijaz

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Reproductive Physiology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/13661/1/Muhammad_Ijaz_Khan_Reproductive_Physiology_2016_HSR_QAU_22.08.2016.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676724948244

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The study was conducted at Livestock Development Research Centre Muzaffarabad, Azad Jammu and Kashmir. The primary objective of this study was to improve the milk production of indigenous cows along with other productive and reproductive traits by crossing with European breeds. The indigenous heifers were artificially inseminated with Jersey semen and F1 crossbred were produced. The F1 offspring were selfed to obtain the F2 offspring and on the other hand the F1 cows were crossed with Frisian bull to produce three-breed crossbred cows. The information regarding productive and reproductive traits of all the cows was studied. The number of cows for each group were 48 for indigenous, 32 for F1 (Indigenous × Jersey) cross, 19 for F2 (F1 × F1) cross and 18 for F1 × Frisian cross. Highly significant increase (P < 0.0001) in milk yield and birth weights of calves was observed in all the crossbred cows compared to indigenous cows. Highest milk yield per lactation (1411.0 ± 92.88 liters) and highest lactation length (354.5 ± 16.70 s) was observed in F1 × Frisian cross whereas the highest 305day milk yield (1674.0 ± 47.58 liters) and daily average milk yield (5.07 ± 0.14 liters) was observed in F1 (Indigenous × Jersey) cows. 305day milk yield did not differ significantly between F2 (1295.0 ± 75.36 liters) and F1 × Frisian (1355.0 ± 60.32 liters) cows. The sex ratio of male and female calves did not differ significantly among the all breed groups (P > 0.05). Similarly method of breeding did not affect the sex ratio of male and female calves. Mean age at first calving (AFC) reduced highly significantly (P < 0.0001) in all the crossbred cows (951.2 ± 37.35 s for F1, 1086 ± 37.89 for F2 and 952.1 ± 28.23 s for F1 × Frisian) compared to indigenous (1861 ± 42.45 s) cows. Mean AFC of F1 and F1 × Frisian cows did not differ significantly (P = 0.9869). Mean dry period of F1 (110.2 ± 4.78 s); F2 (124.8 ± 10.14 s) and F1 × Frisian (99.76 ± 6.67 s) cows decreased highly significantly (P < 0.0001) compared to indigenous cows (239.5 ±7.87 s). The dry period among the crossbred cows did not differ significantly (P > 0.05). The service period recorded in present study was 256.0 ± 8.67, 92.60 ± 5.04, 81.81 ± 11.19 and 266.7 ± 16.56 s for indigenous, F1, F2 and F1 × Frisian cows respectively. The mean service period in F1 and F2 crossbred cows decreased highly significantly (P < 0.0001) compared to indigenous cows but no significant difference (P = 0.5493) was observed between the service period of indigenous and F1 × Frisian cows. Mean calving interval of one year was observed in F1 (368.8 ± 5.32 s) and F2 (359.8 ± 11.68 s) crossbred cows where as a calving interval of 518.6 ± 9.54 and 540.9 ± 22.39 s was observed in indigenous and F1 × Frisian cows respectively. The mean calving interval decreased highly significantly in F1 and F2 cows compared to indigenous and F1 × Frisian cows (P < 0.0001). Mean calving interval of indigenous and F1 × Frisian cows did not differ significantly (P = 0.2895). High breeding efficiency was observed in F1 (93.68 ± 1.85 %) and F2 (93.71 ± 2.74 %) and it increased highly significantly (P < 0.0001) in F1 and F2 compared to indigenous cows (73.46 ± 2.50 %). The mean breeding efficiency of F1 × Frisian (65.62 ± 3.05 %) did not differ significantly from that of indigenous cows (P = 0.0870). Within F2 crossbred cows significant (P < 0.05) sires effects were observed on 305 milk yield, daily milk yield and service period while the birth weight, milk yield per lactation, lactation length, age at first calving, dry period and calving interval were not affected significantly (P > 0.05) by sire. The sire effects within F1× Frisian crossbred cows were not observed (P > 0.05). Parity has significantly affected 305day milk yield in indigenous, F1 and F2 cows whereas parity did not significantly affected 305day milk yield in F1 × Frisian cows (P = 0.2472). The 305day milk yield in indigenous cows decreased significantly (P = 0.0063) from parity one to parity five. 305day milk yield in F1 and F2 increased significantly (P < 0.05) towards 4th and 5th parity and decreased thereafter. The mean lactation length in indigenous cows decreased significantly from first to five parity (P = 0.0024). Parity did not affect significantly (P > 0.05) lactation length of crossbred cows in relation to parity. Season of calving significantly (P < 0.05) affected the 305day milk yield in indigenous and crossbred cows. In indigenous cows autumn calvers produced the highest 305day milk compared to spring, summer and winter calvers. In crossbred cows highest 305day milk yield was observed in winter calvers compared to spring, summer and autumn calvers. The lactation length was not affected significantly (P > 0.05) by the season of calving in nondescript and their crossbred. Year of calving had a significant (P < 0.05) effect on 305 milk yield and lactation length of nondescript indigenous cows. 305 milk yield and lactation length of all the crossbred groups was not affected significantly (P > 0.05) by the year of calving. Overall productive and reproductive performance of indigenous × Jersey (F1) crossbred cows was found to be better compared to F2 and F1× Frisian crossbred cows. Thus upgrading of indigenous cows of AJ&K with exotic breed of Jersey is suggested in this study.
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مُلّا جان محمد

مُلاّ جان محمد
افسوس ہے گذشتہ مہینہ ہمارے دومخلص قومی کارکن ملاجان محمدکلکتہ اورمولانا محمد عاقل الٰہ آباد انتقال کرگئے۔ملا صاحب کااصل وطن پشاور تھالیکن عرصہ دراز سے کلکتہ میں آبسے تھے اوراب سچ مچ وہی ان کاوطن تھا۔نہایت پُرجوش،جری اوربیباک انسان تھے۔گذشتہ نصف صدی میں کوئی قومی اور ملی تحریک ایسی نہیں ہے جس میں انہوں نے بڑھ چڑھ کراورولولہ و عزم کے ساتھ حصہ نہ لیا ہو۔ ان کی علمی زندگی کاآغاز تحریک خلافت سے ہوا اوراختتام مجلس مشاورت پر۔کلکتہ میں شاید ہی کوئی مسلم ادارہ(یہاں تک کہ محمڈن اسپورٹنگ کلب بھی) ایسا ہوجس میں ملاصاحب نے نمایاں حصہ نہ لیاہو۔اسی وجہ سے وہ کلکتہ کے لوگوں میں بے حد مقبول تھے۔ بڑے بے غرض،بے لوث اورنہایت سادہ اورمخلص مسلمان تھے۔۱۹۵۰ء میں کلکتہ کے فساد میں لوگوں نے ان کو بچوں کی طرح چیختے اور روتے دیکھا ہے۔ عمر۸۵ سال کے قریب تھی۔ [نومبر ۱۹۷۲]

 

غیر مسلم اقلیتوں کے حقوق کا اسلامی فلسفہ: قرآنی تناظر میں تجزیاتی مطالعہ

Islamic Philosophy of the Rights of Non-Muslims Minorties: An Analysis in Qur’ᾱnic Perspective Islam is complete code of life for entire humankind. According to the Holy Quran all human beings have been created from a single person (Adam). By birth all are equal and have all fundamental rights irrespective of their religion. If a group is numerically inferior to the rest of the population of state in a non-dominant position, will not be considered minority according to Quran. Quran classify people into two different categories: believers and non-believers on the base of their belief instead of numerical value. Minorities enjoy all fundamental rights and freedoms in Islamic territory. This article demonstrates in the light of Quran that Islam does not discriminate between Muslims and non-Muslims in the matter of rights and will clarify the objection of western propaganda that Islam is rigid for non-believers. There are different types of Non-Muslims living in Islamic state as minority. According to Quran, concept of minority is unique, minority does not mean inferior in number. This paper will reveal the types of minorities and their specific rights along with their basic rights too. It has been concluded that Islam is only religion which offers basic human rights at the level of equality irrespective their religion.

Linkage Analysis of Pakistani Families With Autosomal Recessive Retinitis Pigmentosa

Over the past decades progress in the field of molecular genetics has had an immense contribution to the better understanding of hereditary diseases. Hereditary retinal disorders are a group of diseases that affect the normal function of retina leading to partial or complete loss of eye sight. Depending upon the type and severity of the disease, loss of vision may occur suddenly or gradually. Despite the age of onset and symptoms, eye diseases generally affect the overall quality of life in the affected individuals of all races, cultures and ethnicities and thus remained an active area of research in the past and will be explored in the future as well. The current study focused on the genetic analysis of eight consanguineous Pakistani families (A-H) with multiple members suffering from autosomal recessive RP or retinal dystrophies. These families were enrolled from different rural villages of Pakistan including Punjab, Khyber PakhtunKhwa and Sindh provinces. Clinical data of the affected members of the families were obtained and diagnosis of RP was made after ophthalmic assessment by local ophthalmologist. Physical evaluations ruled out presence of extraocular phenotypes. Blood samples were collected from available members of families and genomic DNA was isolated for use in genetic analysis. Initially all collected families were tested by STS based homozygosity mapping which result in the mapping of family B to chromosome 16. Remaining seven families were subjected to SNP based genome scan which revealed their mapping to different genomic regions. Further follow up of these seven families led to the identification of three novel muta-tions; (c.244- 2A>C) in C8ORF37 (Family C), (c.786delT) FAM161A (Family D) and (g.[152634_42094] delins A) LCA5 (Family F) genes. However mutation analysis of ZNF513, C2ORF71, FAM161A, VSNL1 genes in family A and CLN3 gene in family B did not identify any pathogenic variation. Two families (Family E and H) with multiple homozygous regions and a third family (i.e family A) underwent RD panel based next generation sequencing which only resulted in the identification of a known c.1600G>A in family E in TRPM1 gene. Although we identified two heterozygous variants (c.5653 A>G and c.14662 A>T) in USH2A gene in family A by RD panel sequencing but these variants did not segregate with the disease phenotype in this family. The splice site mutation (c.244 -2A>C) identified in family C was further analyzed with a minigene assay which confirmed the loss of splice acceptor site and the activation of Linkage Analysis of Pakistani Families with Autosomal Recessive Retinitis Pigmentosa xvi Abstract cryptic splice site in exon 3. Sanger sequencing of the cDNA also confirmed the activation of the cryptic splice site within exon 3 which result in the deletion of 22 nucleotides from the RNA. This 22 nucleotide deletion probably results in the frameshift and premature truncation of the protein. DNA walking was used to identify the large LCA5 deletion in family F. Sanger seuencing of PCR products obtained with DNA walking kit revealed a large homozygous deletion of 110540 bps (g.[152634_42094] delins A) in the LCA5 gene. This deletion is predicted to affect the binding site for the basal transcriptional apparatus therefore disrupts the transcriptional regulation and normal gene activation. Family G showed a recurrent mutation c.25G>A in the NMNAT1 gene. While RD panel NGS identified a recurrent missense mutation c.1208G>A, (p.Arg403Gln) in exon 11 of the CNGB3 in family H which did not segregate with the disease phenotype in the family. As this family has been clinically diagnosed with retinitis pigmentosa the CNGB3 variant does not segregate with the disease phenotype therefore negates the disease causative nature of the variant in this family. Families that did not link to any of the known genes/loci by conventional sequencing techniques may have the potential to link to novel genes involved in the pathogenesis of retinal dystrophies. Whole exome sequencing or whole genome sequencing may be implemented to determine the underlying genetic factors for families A, B and H. Linkage Analysis of Pakistani Families with Autosomal Recessive Retinitis Pigmentosa xvii Abstract This study resulted in one publication, · Ravesh and El Asrag et al., 2015. Novel C8orf37 mutations cause retinitis pigmentosa in consanguineous families of Pakistani origin Two manuscripts submitted and currently under review · Ansar and Ravesh et al., 2015. Detection of Novel Mutations Causing Autosomal Recessive Retinitis Pigmentosa in Pakistan · Ravesh et al., 2015. DNA walking reveals a large deletion of LCA5 in a consanguineous family from Pakistan Abstracts Presented in International Conferences · Ravesh Z, Weisschu N, Wissinger B, Ansar M. (2015): Molecular genetic analysis of Hereditary Retinal Dystrophies in Consanguineous Families from Pakistan. (Asia ARVO 2015, Feb 16 - 19, Yokohama, Japan). · Ravesh Z, Weisschu N, Reuter P, Bonin M, Ansar M, Wissinger B. (2015): Molecular genetic analysis of Autosomal Recessive Retinitis Pigmentosa & Leber congenital amaurosis in Pakistani Population (25th Annual Meeting of the German Society of Human genetics, ESSEN 2014).