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Home > Relationship B/W C1418t Segment of Tm Gene Polymorphism With Coronary Artery Disease Patietns in General Adult Population of Karachi, Pakistan

Relationship B/W C1418t Segment of Tm Gene Polymorphism With Coronary Artery Disease Patietns in General Adult Population of Karachi, Pakistan

Thesis Info

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Author

Mughal, Muhammad Akbar

Program

PhD

Institute

Baqai Medical University

City

Karachi

Province

Sindh

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Physiology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11794/1/Muhammad%20Akbar%20Mughal_Physiology_2019_Baqai_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676724968822

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Background Coronary artery disease is one of the leading causes of mortality and morbidity around the world. The Indo-Pak sub-Continent has one of the highest predispositions to Coronary artery disease. Apart from the established risk factors such as diabetes mellitus, hypertension, smoking, alcohol intake, family history, and obesity that played an important role in the development of CAD, genetic polymorphism also emerge as a risk factor in the incidence of Coronary artery disease. Thrombomodulin (THBD) gene is one of them. It is a transmembrane protein expressed on the surface of endothelial cells. It is an important vasoprotective and thromboresistant substance. This natural anticoagulant abates thrombin’s activity and activates Protein C. The relationship between Thrombomodulin gene polymorphisms and coronary disease remains Controversial. It has been reported that a single nucleotide polymorphism (CT) at position 1418 of thrombomodulin gene results in amino acid change from alanine (A) to valine (V) at protein position 455 of TM gene (Ala 455 Val). A considerable number of studies conducted across the globe showed a varied relationship between TM gene polymorphism and coronary artery disease. Therefore the aim of the present study is to assess the association between TM gene Polymorphism and coronary artery disease in general adult Population of Karachi, Pakistan ii METHODOLOGY This is a case control study that was conducted in Tabba Heart Institute in association with National Institute of Blood Disease, Karachi. In this study we explore the association between C/T dimorphism that might predict as a risk factor for coronary artery disease in population of Karachi. We compared C/T dimorphism in 92 cases with 90 control subjects by allele specific amplification. RESULTS The frequency of CC, CT and TT genotype was 81 (90 %), 6 (6.7%) 3 (3.3%) in controls and 67 (72.8%), 20 (21.7%) and 5 (5.4%) in cases respectively. In case group the CT/TT genotype were found to be significantly associated with coronary artery diseases when compared with control group (p-value 0.009). Both cases and control were in Hardy Weinberg equilibrium (HWE), i.e. for cases HWE was (X2 = 3.81, p>0.05) and for controls HWE was (X2 = 19.4, p>0.05). CONCLUSION TM C1418T polymorphism appears as a risk marker in Coronary Artery Disease patients in the population of Karachi, Pakistan.
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شاعرانہ رنگ

شاعرانہ رنگ

                وہ بذات خود کیونکہ شاعر بھی ہیں اس لیے انہوں نے اپنے ناول میں نثر کے ساتھ ساتھ اپنی شاعرانہ طبیعت کو بھی زندہ رکھا ہے۔ نثر میں ان کا اندازبہت سادہ اور صاف ہے۔ نفیس طریقے سے لکھے گئے جملے سیدھے دل میں اترتے ہوئے محسوس ہوتے ہیں۔ بالکل ویسے ہی جیسے ان کے دل و دماغ سے نکلے ہوئے ہوں۔

                اپنی شاعرانہ طبیعت کے پیش نظر انہوں نے اپنی تخلیقی قوتوں سے ناول میں اپنی شاعری کے جوہر دکھائے ہیں اور ناول کے حسن کو چار چاند لگا دیئے ہیں اور ناول میں جہاں جزئیات نگاری سے کام لیا گیا ہے۔ طوالت کو مد نظر رکھا گیا ہے وہاں شاعری سے ٹھندی ہوا جیسی طراوت محسوس ہوتی ہے۔ نو لکھی کوٹھی میں شامل ایک نظم، جس نے سارا ماحول بدل کر رکھ دیا، اس کا کچھ حصہ ملاحظہ ہو:

"نومبر ہمیشہ اداس ہوتا ہے

رکا ہوا، مطمئن اور بے نیاز

اس کی وادی میں صبح ہوتی ہے، دوپہر سہ پہر

پھر شام آ جاتی ہے

مگر دھوپ کا مزاج نہیں بدلتا

آسمان کی طرح پر وقار بزرگی والا

زندگی نومبر کی طرح نہیں

زندگی بدلتی ہے، متواتر بدلتی ہے

وہ تجھے نومبر میں نہیں رہنے دے گی

دھوپ غبار آلود ہو جائے گی

 صاف نظر آنے والی چیزیں دھندلا جائیں گی

پھر سیاہ ہو جائیں گی

پھر اندھیرا کھا جائے گا

اس وقت جب میں نہیں ہوں گا

دوست کوشش کرنا نومبر نہ گزرے

وکالت کی تحقیق فقہاء کرام کی نظرمیں

There has been described the meaning of Wakalat and its type. Who can be agent? What are the conditions of it? How it can be used? Where it is used? How it can be eliminated? What is the status of it in Current kinds of Cards? In which things the wakalat will be eligible. Allah has permitted for wakalat as it was mentioned in this article, because it is a necessary need of a human being without it one never can do all around his works, issues and important goods. For Wakalat it is very credible that Wakeel must has experience in the relevant subject without experience he can give countless loss for his Mowakkil, as well as be eligible, trustful, honest, sensible, aware of current affairs. Existence of wakalat has been described by all jurispru-dence and religious scholars divided Wakalat in two types (1) common Wakalat (2) special Wakalat and their command according to the holy Quraan and sunnat. What is the command of wakalat in almighty Allah’s affairs? Is it allowed or prohibited according to the Islamic point of view.  

Influence of Indigenous Cultures and Ripening Temperatures on Cheddar Cheese Quality

Cheddar cheese cultures were isolated and purified from indigenous sources using various microbiological techniques and characterized on basis of sugar fermentation, biochemical and enzyme activity tests, growth at various temperatures, pH and NaCl concentrations and using API identification kits. Raw milk and sour cream were found the most suitable indigenous sources for isolation and purification of Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. cremoris. The later was differentiated from the former by no acid production from maltose, ribose, mannitol, arabinose, inositol, trehlose, negative arginine hydrolysis test, no growth either in 4% w/v NaCl or at 40°C and pH 9.2. Commercially available and locally isolated strains of Lactococcus lactis ssp. cremoris and Lactococcus lactis ssp. lactis were propagated in combination of 95:5 to prepare the mother cultures. These mother cultures were employed in standardized buffalo and cow’s milks to manufacture Cheddar cheese and ripened at 4°C and 12°C for 120 days. During ripening, cheese was evaluated for physico-chemical composition and sensory quality. Buffalo milk cheese contained significantly higher fat, protein, ash, lactose, sodium, calcium, potassium and organic acids contents; particularly lactic, citric and butyric acid contents as compared to cow. Cheddar cheese prepared from indigenous cultures had significantly lower lactose and pH and higher acidity than commercial cultures. The concentrations of all the organic acids produced by indigenous cultures were distinctly higher than commercial cultures. During ripening, lactose and pH decreased while acidity increased significantly. The concentrations of all the organic acids increased highly significantly during ripening and elevated temperature considerably accelerated the production of all organic acids except pyruvic acid. Starter cultures and ripening temperatures substantially influenced the aldehydes, ketones and alcohols, while milk composition considerably affected the alcoholic and sulphur compounds. Descriptive sensory evaluation indicated that cheese samples prepared from buffalo milk using indigenous cultures were scored significantly higher for most of the attributes. Elevated temperature perceptibly accelerated the development of odor, flavor and texture characteristics during ripening. Hence, it is concluded that indigenous starter cultures and buffalo milk produced the cheese with improved quality and elevated temperature accelerates the process of ripening.