The way an individual performs is important for both; the organization as a whole and the employees working in it on an individual level. The behavioral and the outcome aspects constitute to form the concept of performance. In order to pursue their goals and reach their destination, organizations need individuals who perform to their utmost capacity, in order to perform their responsibilities towards their products and services they have gained expertise over and ultimately differentiating themselves from their competitors. Hence, organizations have started to take greater interest in the causes underlying the reasons for declining work performance. Therefore theoretical perspectives from psychology and organizational behavior supported with neurocognitive proof has been integrated in this research, for the sole purpose of examining the effects of sleep deprivation on the performance of working mothers with in educational sector of Pakistan. In particular, it has been argued that there is a negative relationship between sleep deprivation and employee performance and the given relationship is partially mediated by workplace deviance and stress at the workplace. Specifically, sleep deprivation was expected to increase anxiety among employees, and results in deviant behaviors at the workplace, which would in turn decrease the overall performance of working mothers ultimately. Personal survey technique has been applied here as a method of data collection and questionnaire has used as an instrument to collect the data. Sample size used for this study was 377 respondents. The sample has been taken from working mothers who are teaching and giving their services in different organizations of educational sector of Pakistan. Based on the findings and hypothesis results it can be concluded that the given negative relationship between sleep deprivation and employee performance is partially mediated by workplace deviance and stress at workplace