حکیم محمد سعید
حافظ حکیم محمد سعید کے وحشیانہ اور بے رحمانہ قتل پر کون ہے جو تڑپا اور بے قرار نہ ہوا ہوگا، وہ بین الاقوامی شخص، مملکتِ خداداد کے ہمدرد و مسیحا، پاکستان کے معمار، اس کا مقدر چمکانے اور بنانے کے لیے فکر مند، ہمدرد فاؤنڈیشن کے سربراہ اور مدینتہ الحکمت کے بانی، عالم، مصنف، مدبر، عالی دماغ، سچے اور پکے مسلمان، قوم و ملت کے بے لوث خادم، مخالف ہوا میں طب کا چراغ روشن کرنے، علم کو فروغ دینے، حکمت و محبت کا خزانہ لُٹانے والے، غریبوں اور مصیبت زدوں کے درد و غم میں شریک، پہلو میں درد مند دل رکھنے، پاکستان کے تشویش انگیز حالات سے بے چین اور پریشان رہنے والے کو چند دقیقوں میں جن لوگوں نے موت کے گھاٹ اُتارا وہ بڑے خوں خوار، درندہ صفت اور انسانوں کے بھیس میں وحوش سے بھی بدتر لوگ تھے۔ آخر اس فرشتہ صفت، پاک دل، پاک باز، رحم و مروت کے پیکر، مجسم شرافت اور اخلاقی خوبیوں سے منور انسان کا قصور کیا تھا، ذوق مرحوم کے شاگرد محمد مظفر خاں گرمؔ رامپوری کا وہ قطعۂ تاریخ وفات یاد آگیا جو نواب شمس الدین خاں مرحوم کو پھانسی دیے جانے پر کہا گیا تھا
یہ دست درازیِ ستم کس سے بیاں ہو
بے جرم و گنہ مسند نواب کو الٹا
تاریخ معمے میں نئی طرز سے لکھ، گرمؔ!
کیا چرخ نے ’’نوابی سہراب‘‘ کو الٹا
جو مملکت اُس اسلام کے نام پر وجود میں آئی تھی جو سراپا امن و سلامتی ہے اور جس کے آئین میں خون خرابہ اور قتل و دہشت گردی کی کوئی گنجایش نہیں اور جس کے ماننے والے (مسلم) ساری دنیا کے لیے سراپا رحمت ہوئے ہیں، آج وہی مملکت جہنم کا نمونہ بنی ہوئی ہے، کراچی مقتل میں تبدیل ہوگیا ہے، پاکستان...
Muhammad’s (SAW life is fully reserved and recorded in history. It has been established beyond a shadow of doubt that not only the major and significant events of his life but even the minutest details of his life are also painstakingly preserved by the Muslims. In spite of the availability of abundant authentic documents concerning Muhammad’s (SAW) life, sayings and deeds, some critics of Islam stubbornly refuse to believe in his historicity. Robert Spencer is one such critic who maintains that Muhammad’s (SAW) value is nothing more than a fictional or mythological figure. This article is an attempt to prove the existence of Muhammad (SAW) in contemporary non-Muslims sources. The article is divided into three major parts: the first part of the paper throws light on how much emphasis Muslims lay on historical authenticity of Muhammad (SAW), in the second part of the paper views and doubts of prominent like orientalists Spencer concerning Muhammad’s (SAW) historical authenticity have been summarized, whereas the third part presents Robert Spencer’s views in the same connection and endeavors to refute his views and approach drawing on contemporary non-Muslim Sources.
Staphylococcus aureus is the main cause of mastitis in dairy goats. This organism is recognized as major mastitis causing pathogen in dairy animals worldwide. In humans, it also caused various diseases such as food poisoning and Staphylococcal enterotoxins. This bacterium mostly infected the dairy herds due to poor sanitary conditions and untreated groundwater used to wash utensils during milking practice. The purpose of this study was to determine the prevalence of Staphylococcus aureus among infected dairy goats. Hundred milk samples were collected from infected dairy goats and grown on a blood agar. A total of 58/100 (58%) were positive for S.aureus that was further confirmed on the basis of biochemical tests namely catalase positive and coagulase positive. The positive isolates of S. aureus strains were analyzed using a molecular technique. DNA of positive S. aureus isolates was extracted using Macherey-Nagel kit method. The extracted isolates of S. aureus were run on agarose gel electrophoresis. Ethidium bromide dye was used to visualize DNA bands using UV illuminator. The amplification of genes encoding 16S rRNA of positive S. aureus isolates was performed by Polymerase chain reaction. Primers (Forward primer 5''-AGA GTT TGA TCC TGG CTC AG & Reverse primer 5''-AAG GAG GTG ATC CAG CCG CA) were designed using Primer 3 software. PCR clean up method was used in purification of sequences. These amplified gene products were further sequenced by using Sanger method. The sequenced results of these bacterial species were confirmed by comparing the sequences present in BLAST program at National Centre of Biotechnology Information. MEGA 7 software was applied for the construction of Phylogenetic tree. Some other bacterial species such as Staphylococcus epidermidis, Staphylococcus hominis, Enterobacter cloacae and Acinetobacter baumannii along with Staphylococcus aureus were also identified due to their genetic differences. The evolutionary history was inferred using the Neighbor-Joining method. The evolutionary distances were computed using the p-distance method and in the units of the number of base differences per site. The analysis involved 14 nucleotide sequences. There were a total of 371 positions in the final dataset. Antibiotics such as penicillin, erythromycin or tetracycline are used in the treatment of mastitis in goats. It is concluded that Staphylococcus aureus was more prevalent in the milk samples of infected goats and is an etiologic agent of mastitis in dairy goats. The consumption of raw milk may infect humans and lead to various infections.