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Home > Studies on the Isolation and Characterization of Secondary Metabolites from Dodonaea Viscosa and Quercus Baloot and Their Potential As Antibacterial Agents

Studies on the Isolation and Characterization of Secondary Metabolites from Dodonaea Viscosa and Quercus Baloot and Their Potential As Antibacterial Agents

Thesis Info

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Author

Khurram, Muhammad

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Subject

Agricultural Technology

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/330

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725385890

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The present studies were aimed to isolate and characterize the secondary metabolites responsible for antibacterial activity from medicinal plants present in Pakistan. Two plant species Dodonaea viscosa (L.) Jaeq. and Quercus baloot Griff. were selected on the basis of literature review and their traditional uses in ailments related to microorganisms. The n-hexane, dichloromethane, ethyl acetate, n-butanol and aqueous fractions of Dodonaea viscosa were analyzed for antimicrobial potential against four Gram positive bacteria: Bacillus subtilis (MRL M 1), Bacillus cereus (MRL M 52), Micrococcus luteus (ATCC 10240), Staphylococcus aureus (ATCC 6538); three Gram negative bacteria: Escherichia coli (ATCC 25922), Salmonella typhi (Cl. I. 140), Pseudomonas aeruginosa (ATCC 9721) and the yeast Candida albicans (Cl. I. 4043). It showed inhibition against S. aureus, M. luteus, B. subtilis, E. coli, P. aeruginosa and C. albicans. The TLC solvent systems for each of the fraction were developed and the resulting chromatograms of the fractions were sterilized using ethylene oxide or dioxane, which were then subjected to contact bioautography. Multiple inhibition zones were observed at different R f values against B. subtilis, M. luteus, E. coli, S. typhi, P. aeruginosa, and C. albicans indicating the presence of antimicrobial components. Isolation of the active principles responsible for the antimicrobial activities was attempted through preparative TLC, but it was unable to yield the compounds. The results from the preliminary screening and contact bioautography indicated n-hexane, ethyl acetate and n-butanol fractions having good potential in terms of antimicrobial activities, therefore, they were chosen for further investigations. HPLC revealed the presence of large number of metabolites; therefore, further isolation was done using preparative HPLC that resulted in 52 sub-fractions each for n-hexane and n-butanol fractions while 48 sub-fractions were obtained from ethyl acetate fraction. XTT-Bioassay was used in hyphenation with preparative HPLC to mark the antibacterial potential of the emerging sub-fractions. S. aureus (NCIMB 6571) and E. coli (NCIMB 8797) were used in all XTT based bioassays. On the basis of bioassay results, six sub-fractions from n-hexane fraction were selected and analyzed upon HPLC in analytical mode, which indicated multiple numbers of compounds in them, thereby, necessitating further isolation. Further fractionation gave 218 sub-sub fractions that were tested against the same two bacteria. The sub-sub fractions indicating antibacterial activity were analyzed upon HPLC and isolation of the compound was possible from the sub-sub fraction no. 12 of sub-fraction 42 of n-hexane fraction of D. viscosa’s crude ethanolic extract. The compound’s MIC’s against S. aureus (NCIMB 6571) and E. coli (NCIMB 8797) were 64 μg/ml and 128 μg/mlxviii respectively. The MBC’s against these organisms were 128 μg/ml and 256 μg/ml, respectively, which indicated a moderate activity against the Gram-positive bacterium. The structure analyses revealed the compound to be 15, 16-epoxy-cis-cleroda-3, 13(16),14- trien-18-oic acid-18,6-olide, a clerodanefuranolactone, previously known for its structure but this is the first report of its antibacterial potential and its presence in D. viscosa. Quercus baloot fractions were processed in the same manner and were subjected to antimicrobial analysis using similar panel of microorganisms. Preliminary screening using disk diffusion and agar well diffusion methods showed inhibition zones against S. aureus, M. luteus, B. subtilis, E. coli, P. aeruginosa and C. albicans. TLC chromatograms and subsequent contact bioautography showed inhibition zones at different R f values against B. subtilis, M. luteus, E. coli, and S. aureus indicating the presence of antimicrobial components. On the basis of these findings QDM fraction underwent HPLC evaluations that indicated a good number of metabolites; therefore, preparative HPLC was carried out that yielded 52 sub-fractions that were subjected to XTT bioassay to mark the antibacterial potential from which five potential sub-fractions were again analyzed upon HPLC. Each sub-fraction had several compounds, thereby; preparative HPLC was applied that resulted in 175 sub-sub fractions, which were subjected to XTT bioassay using same two bacteria. The sub-sub fractions indicating antibacterial activity were analyzed upon analytical HPLC and isolation of a semi-purified compound was made from the sub-sub fraction no. 15 and 16 of sub-fraction 39 of dichloromethane fraction obtained from Q. baloot’s crude methanolic extract. Its MIC’s against S. aureus (NCIMB 6571) and E. coli (NCIMB 8797) were 16 μg/ml and 128 μg/ml respectively while MBC’s were 64 μg/ml and 256 μg/ml, respectively. This compound requires further purification and characterization. This is the first report of such an activity in Q. baloot.
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پیر احسان اﷲ شاہ

پیر احسان اﷲ شاہ
علمی حلقوں میں یہ خبر غم و افسوس کے ساتھ سنی جائے گی کہ چھندم گوٹ ضلع حیدرآباد سندھ کے مشہور عالم پیر احسان اﷲ شاہ صاحب جو قلمی کتابوں کے بڑے عاشق تھے چوالیس برس کی عمر میں ۱۳؍ اکتوبر ۱۹۳۸؁ء کو اس دنیا سے چل بسے، مرحوم حدیث و رجال کے بڑے عالم تھے، اور ان کے کتب خانہ میں حدیث و تفسیر و رجال کی نایاب قلمی کتابوں کا بڑا ذخیرہ تھا، ان کے شوق کا یہ عالم تھا کہ مشرق و مغرب مصر و شام و عرب و قسطنطنیہ کے کتب خانوں میں ان کے کاتب اور ناسخ نئی قلمی کتابوں کی نقل پر مامور رہتے تھے، مرحوم ایک خانقاہ کے سجادہ نشین اور طریق سلف کے متبع، اور علم و عمل دونوں میں ممتاز تھے، اﷲ تعالیٰ مرحوم پر اپنے انوار رحمت کی بارش فرمائے۔ (سید سلیمان ندوی، نومبر ۱۹۳۸ء)

 

Conditions and Qualification for Being a Judge in the Light of the Islamic Law

‘Adl and Qisṭ is indeed a manifestation of God’s mercy, rather, it may also be seen as a principal objective of Shariʿah. The origin or sources of administration of justice in Islām are the Quran, Sunnah of the holy Prophet, consensus of opinions of the jurists of Islamic jurisprudence and Analogy (Qiyās). Justice is a sacred obligation of supererogatory. It is obligatory upon the Muslim rulers to appoint judges for the dispensation of justice to attain equality, to protect the human rights from their violation, to safeguard the lives and properties, and to maintain law and order in society. As a judge is supposed to accomplish a very important and noble task being a regent of Allāh, hence, some vital merits and criteria regarding the conditions and qualifications for the appointment of the Qāḍī or judge in the light of the Qur’ān, Sunnah and Islamic jurisprudence and the code of conduct for the Pakistani judges must be observed at all costs. The author of this paper has discussed these conditions and qualifications in this article. There are some unanimous conditions for the appointment of judges, while some others are not agreed upon. While presenting the difference of opinions of the Islamic jurists, the author tried to explain, reconcile the opinions and at some places presented her own view in the light of her analysis and arguments. These conditions are around thirty, but the author according to her own discretion chose some of the most important ones to discuss in this paper.

اردو ادب میں معاصرانہ چشمک

The first movement of Urdu Literature raised its voice in Fort william college which bases remained beyond theoritical reference Sir syed''s revolutionary objectivity was upheld opponent hand of many writers claimed their supremecy against him and contemporary animosity paved its avenues among these movements Whenever the Literature griped by individuality substanardisation shaked its foundation The first chapter of the thesis is''''The biginning of Urdu Language and Literature'''' which showed urdu language with its background. Initial phases and reasons of growth of language are described.All combats and adventures in the name of provinces in Indo-Pak continent are emphasized. This chapter also deals with the writer''s idealogies to comments the connections between Punjab,Sindh,Dakkan,Delhi,Bangal,Mysur, Behar,Gujrat,Potohar,Madras and other regions controversial idealogies in the beginning of language is discussed here.During British rule in Hindustan,Orientalist initiated conflicts between Hindus and Muslims on the bases of Hindi,Urdu and Hindustani which was strenthened by prejudice Hindus that germinated on endless controversy.This chapter describe the whole penorama and also elaborates the conflict revolving around the style of expression. The second chapter is titles as ''''Contemporary animosity in poetry and Literature''''that reflect the colours of urdu poets and written controversal discussions with each other on the ground of language ,words and style.The disciplin of poetry,art of language and the excellence of expression are the subject of this chapter. The third chapter deals with literary movements with their literary and historical back ground .The movement like the earliest urdu poetry,ambegous movement,Fort william college,Ali garh movement,Romantic movement,Progressive movement,Islamic movement,Pakistani literatur and some other movement are discuss in this chapter The fourth chapter represent the contemporary animosity of Journals and the editors of Journals,that develops from Tehzeeb ul akhlaq and leads towards 21st century when written controversal discussions were published in different magazines The fifth chapter name as ''''personaly and individualy animosity'''' the richest chapter of the thesis.Famous literary adventures are its soul the competition between poetry and prose as one to one are not negated.Literary achievements of meer and Sauda,Insha snd Mushafi,Nasikh and Aatish, Anees and Dabeer, Ghalib and Zauq,Abdul majid and Aazad, Qasmi and Wazir aagha,Narang and Farooqi,Salim akhtar and Anwar sadid, Himaet ali shaer and Mohsin bhopali,Allama Iqbal,Fraz and Iftikhar arif and others can never be neglected besides this the unique competition between old and new contrmper poets and writers are vitally added to the chapter.As one couplet of ghazal to be the cru this chapter is the soul of this thesis. The last chapter of the thesis is ''''The complete analysis of contemporary animosity in urdu literature'''' which manifests observations and significances of the little and the work undone are also dealts without selecting options the splended vastness and the side effects of the merit relevent are precisely discuss.