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Home > Agrobacterium Mediated Transformation and Secondary Metabolite Analysis of Ajuga Bracteosa Wall. Ex. Benth

Agrobacterium Mediated Transformation and Secondary Metabolite Analysis of Ajuga Bracteosa Wall. Ex. Benth

Thesis Info

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External Link

Author

Rani, Rehana

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7753/1/Dr%20Rehana%20thesis.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725444161

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Ajuga bracteosa is a valuable yet endangered medicinal plant containing many important compounds, including withanolides, phytoecdysteroids, neo-clerodane and iridoid glycosides. These compounds possess a broad spectrum of biological, pharmacological and medicinal properties, but their yield is very low in wild A. bracteosa and chemical synthesis is not viable. The increased demand of natural products for medicinal purposes coupled with the low product yields and supply concerns of plant harvest has renewed interest in large-scale in vitro plant culture technology. A.bracteosa plant was subjected to in vitro culturing with the aim to study the effect of plant growth regulators on somatic embryogenesis for mass production and uniform plantlets for further genetic transformation. Leaf explants cultured on B5 medium containing 1mg/L 1-naphthaleneaceatic acid (NAA) + 0.5 mg/L 6-Benzyl amine purines (BAP) proved to be the most effective combination for somatic embryo induction. Agrobacterium tumefaciens strain GV3101 harboring pPCV002-A and pPCV002-CaMVC was used to obtain transgenic A. bracteosa plants. Transformation efficiency of 38% for rolA gene and 31% for rolC was obtained. Molecular analysis such as PCR and Southern blotting confirmed the integration of the rolA and rolC genes in the putative transgenic lines. The transcripts of these two genes were monitored by semi-quantitative RT-PCR. Furthermore, a significant difference in phytochemistry and the pharmacological activities (antioxidant, antibacterial, antiproliferative and antidiabetic) was observed not only between the transgenic and control/untransformed plants but also among the transgenic lines themselves. The rolC transgenic lines in general showed better results compared to rolA. Quantitative analysis of Electro-spray ionization mass spectrometer (ESI-TOF) revealed relatively higher contents of 20 hydroxyecdysone (20-HE) in transgenic plant lines of rolA and rolC compared to control/untransformed plant. Effect of low temperature stress on 20-HE biosynthesis revealed relatively high 20-HE content at 15°C compared to control (25°C). Principal component analysis (PCA) of the metabolic profiles obtained with non-targeted (ESI-MS) approach successfully distinguished the control A. bracteosa from the transgenic rolA and rolC population. Defense related secondary metabolites (tryptophan, mannitol, Cinnamic acid, and Shikimic acid) were identified in transgenic A. bracteosa lines. Taken together, our study suggests that low temperature stress and transformation with rol genes can be used for increased, constant and permanent production of valuable secondary metabolites from A. bracteosa plant.
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10 پنجواں شکار

پنجواں شکار

                شکندر بارہویں جماعت دا طالب علم سی اک دیہاڑے جدوں اوہ کالج اپڑیا تے اوہنے ویکھیا پئی کالج دے نیڑے خالی میدان وچ خانہ بدوشاں دیاں پکھیاں لگیاں ہوئیاں نیں کالج لگن وچ اجے کجھ چررہندا سی پئی اوہ بے وس ہوکے پکھیاں ول ٹرپیا جدوں اوہ اک پکھی دے کول اپڑیا تے بت بن کے کھلوتا رہ گیا ایس پکھی وچ اک کڑی چھج بناون وچ لگی ہوئی سی اوہنے سدھے سادھے کپڑے پائے ہوئے سن اوہدیاں گلہاں نوں اک لٹ لگدی پئی سی۔ موٹیاں موٹیاں اکھاں، گول گپا جہیا چہرہ۔ اچن چیت اکو واری ایس کڑی نے شکند رول ویکھیا دونواں دیاں اکھاں چار ہوئیاں تے فیر اوس کڑی نے سر تھلے کرلیا خدا جانے کدوں تیکر شکند راوہدے دل ویکھدا رہیا جدوں کالج دا ویلا ہوگیا تے اوہ مجبور ہوکے کالج ول ٹرپیا پر اوہ چنگی طراں کالج وچہ پڑھ نہ سکیا ہر ویلے اوس کڑی دے خیالاں وچ ڈبیا رہیا۔

                جدوں کالج توں ویہلا ہوئیا بے وس اوہدے قدم اوس پکھی ول اٹھ پئے جیہدے وچ اوہ مٹیار بیٹھی ہوئی سی۔ جدوں شکندر اوتھے اپڑیا تے اوہ وی پہلے توں اوہدی اڈیک وچ سی تے کہن لگی ’’تسیں آگے اوباؤ جی، میں تہاڈی ای اڈیک وچ بیٹھی سی‘‘ ایہہ گل سن کے شکندر حیران ہو گیا اوس کڑی نے دوجی وار فیر آکھیا۔

Educational Philosophy Imam Al-Ghazali’s Perspective

Islam is a divine religion. It is based on divine revelation (Holy Quran) and sunnah of the Holy Prophet ﷺ. As a religion it is a complete code of life. It does not deal with worships only but addresses all fields of life. Like Beliefs and worship, Islam focuses on education also. As a last and chosen religion, it motivates human beings to seek knowledge. The first word of the first revelation (Chapter Al-alaq) starts with Iqra means Read. In first five ayat of chapter Al-alaq, the basic requirement for enhance of education (Read, knowledge and pen) have been mentioned six times. Similarly, the Holy Prophet r took many steps for imparting education. In this connection, the example of first residential university (Suffa’h) is sufficient. Imam Ghazali one of the most famous Muslim thinkers discusses the education in his books in detail. He was born in 448 AH (1057 CE) at Tabaran a town in the district of Tus, which lies within the Khorasan Province of Iran and died on 18 December (1111 CE). In this article knowledge, its classification, stages, curriculum, art of teaching, responsibility of both teachers as well as students have been discussed in the light of Imam Ghazali educational philosophy.

Exploration of Teaching Practices of Reading Comprehension Skills at Public Schools of Gilgit-Baltistan

Teaching English language to the students, and engaging them in the learning tasks especially non-natives, is not an easy task for the teachers in public schools of Pakistan.Teachers of English generally use Grammar Translation Methods (GTM) to teach English which affects the language skills of the students like listening, reading, writing and speaking skills. Various other factors also come into play when English language is taught to non-natives like pedagogical skills of the teachers who are also non-native speakers of English. Keeping this in view, this study was planned to explore the current teaching practices of English language teachers and how they engage students in reading comprehension skills in the context of public schools in rural areas of Gilgit-Baltistan. Qualitative study approach of case study was used to explore and observe the teaching practices of the two English language teachers teaching at grade 6. Data was collected through interviews, classroom observations, document analysis, and field notes. Likewise, focus group discussions were also arranged with the students to look at teachers teaching practices from students’ perspectives. The cases of two English language teachers revealed that they were teaching reading comprehension using GTM. That practice was not helpful for the students to get engaged in the process of reading comprehension.The teachers were not engaging students with reading comprehension strategies such as skimming, scanning, questioning, guessing and meaning making. Participant teachers taught English as a subject, but not as a language. The findings of the interviews and observations imply that teachers of English require the teaching skills of language such as reading comprehension. English teachers need support to learn how to engage students in reading comprehension skills in order to make their teaching purposeful.