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Home > Aloe Vera Polysaccharides and Proteins As Biological Response Modifiers and Their Therapeutic Efficacy Against Coccidiosis in Chickens

Aloe Vera Polysaccharides and Proteins As Biological Response Modifiers and Their Therapeutic Efficacy Against Coccidiosis in Chickens

Thesis Info

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Author

Khaliq, Kashfa

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7810/1/Kashfa%20Khaliq%20Par%20final%20Full%20%20thesis%20pdf.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725460841

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There are several natural biological products, which are effective to trigger immune responses in animals and human beings. In this regard, various plant species are considered to be potent natural biological products and their efficacy has been reported in various animal models. In the present study, Aloe (A.) vera derived biomolecules including polysaccharides and proteins were isolated and characterized as biological response modifiers and their subsequent protective effects against coccidiosis in chickens. A total of 640 chicks were randomly divided into two main treatment groups namely A (polysaccharides) and B (proteins), each containing 320 chicks. Each group was administered orally with the graded doses of polysaccharides and proteins for three consecutive days i.e. day 5th, 6th and 7th of age. Cellular immune responses were assessed for in vivo and in vitro lymphoproliferative responses to Phytohemagglutinin-P (PHA-P), Concanavalin-A (Con-A) respectively and Carbon particle clearance assay. Humoral immune responses were detected by microplate haemagglutination assay on 7th and 14th days post primary and secondary injections of sheep red blood cells. Weekly weight gains and feed conversion ratios were also calculated. For therapeutic studies chickens were challenged with mixed Eimeria species on 14th day post administration of A. vera biomolecules. Results revealed significantly higher (p<0.05) lymphoproliferative response to PHA-P and Con-A in chickens administered with A. vera biomolecules as compared to control. Carbon particle clearance assay showed significantly higher clearance index (K) in control group than all biomolecules administered groups and phagocytic index (α) showed significantly higher response in all three biomolecules administered groups as compared to control. Significantly higher total Igs, IgG and IgM titers were also detected in groups administered with A. vera biomolecules. Biomolecules administered chickens showed better feed conversion ratios and significantly higher (p<0.05) weekly weight gains as compared to control. In challenge experiment maximum protection 70% and 57.5% were observed in polysaccharides and proteins administered groups, respectively. Significantly lower oocysts per gram of droppings, lesser lesion scores, better weight gains and higher anticoccidial index were observed in biomolecules administered groups. From the current study, it was concluded that A. vera derived biomolecules have the potential to be used as immunotherapeutic agent(s) in poultry and can be commercialized.
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’’نیل کے سنگ‘‘پر ایک نظر

نیل کے سنگ ‘‘پر ایک نظر

                                                                                                                ایڈوکیٹ بشیر مراد

                                                                                                                                آزاد کشمیر

شاعری کے لیے کچھ نہیں کرنا پڑتا یہ کھڑے کھڑے ہو جاتی ہے۔ بعض اوقات تو سوئے سوئے بھی سر زد ہو جاتی ہے کہ اس کا تعلق آمد سے ہے جس کے لیے جامد ہونے کی قطعاً ضرورت نہیں۔ لیکن نثر کا معاملہ الگ ہے۔ اس کے لیے باقاعدہ اکڑوں ہو کے بیٹھنا پڑتا ہے۔ یعنی یہ وہ ریاضت ہے جو حالت رکوع میں کی جاتی ہے۔

نثری اصناف میں سفر نامہ تو اور بھی جوکھم کا کام ہے کہ اس کی طلب میں سفر بھی کرنا پڑتا ہے جو بسا اوقات انگریزی کا Suffer ثابت ہوتا ہے۔ گویا یہ ادب کی ایسی صنفِ گراں بار ہے جس کو صنفِ نازک کی طرح قابو کرنا آسان نہیں ہوتا۔ اس کے حصول کے لیے شدید مارا ماری اور بے تحاشا جتن کرنے پڑتے ہیں۔ پہلے سفر کرنے کا سلیقہ اور پھر اسے بیان کرنے کا قرینہ ہونا چاہیے۔

ہمارے دوست ڈاکٹر یوسف میر کی محبت کہ ڈاکٹر الطاف یوسف زئی کا مصری سفر نامہ ارسال کیا۔ مشاہدہ عام ہے کہ یوسف نام کے لوگ خوب صورت ہوتے ہیں۔ پھر بندہ ذات کا بھی یوسف زئی ہو اور مصر سے محبت نہ ہو یہ کیسے ممکن ہے۔ پس یہ طے ہے کہ یہ سفر نامہ سراسر فطری محبت کا شاخسانہ ہے۔ اگر یقین نہ آئے تو پڑھ کر دیکھ لیجیے۔

کچھ سفر نامے تو محض سفر کی صعوبت برادشت کرنے کی خاطر لکھے جاتے ہیں جن کے پڑھنے سے قاری کو بھی شدید تھکاوٹ ہوتی ہے۔ اس قبیل کے سفر نامے یوں شروع ہوتے ہیں۔

’’میں صبح سویرے جاگا ،...

Exploring the Role of “Jihad” in the Establishment of Universal Peace

Jihad is an umbrella concept of Islam, but unfortunately, nowadays it is misused both as a terminology and practice in a very narrow sense. The concept of jihad is crystal clear in Islam but it has been made dubious by certain schools of thought for achieving their vested interests. Consequently differences of opinions about the definition of jihad emerged not only among the masses but also in the circles of the learned ones, hence, no agreed upon definition emerge. Due to this, questions are raised like whether jihad is synonymous to terrorism? Or what is the fundamental role of jihad in establishing universal peace? What is the modus operandi of jihad? But so far, no scholarly effort has been made to hammer this issue out in an unbiased and objective manner. Whatever has been done consist more on either argumentative encounter or defending one’s own ideological stance. For that reason this research article attempts to carry out an objective analysis of the role of jihad in the establishment of universal peace. It is hoped that this attempt of intellectual pursuit will not only be a source of guidance to the common people but will also open new avenues for research scholars in this field.

Theoretical and Empirical Comparisons of Cointegration Tests

During the last three decades, a variety of cointegration tests has been developed to investigate the long-run relationship between two data series. All these tests are based on different characteristics/structures of original time series. These tests were assessed for their performance on basis of their size and power properties using Monte Carlo simulations in a number of studies like, Banerjee, Dolado et al. (1986), Kremers, Ericsson et al. (1992), Haug (1996), Mariel (1996), Österholm (2003), Gabriel (2003), Pesavento (2004) and many more. However, these studies did not give a decisive conclusion that which test is performing better than rest. This is due to variant features of underlying series and every test has its domain of weaknesses and strengths. As it is the basic principle of comparison that tests can be compared on basis of their power properties only when all of tests have same size or stable size around nominal size. However, all of these comparisons were carried out using asymptotic critical values and their sizes were not same and stable. As almost all of comparisons were based on Monte Carlo simulations and the design of Monte Carlo supports the assumptions of model and in real data one doesn’t know whether the assumptions of model hold or not, so there was a gap in literature that these tests may be compared on basis of their size and power properties in a universal set of alternatives using Monte Carlo methods and also these tests may be compared on basis of some real economic data. To fill this gap, this study was carried out in which 24 cointegration tests were assessed for their performance. Out of these 24 tests, 16 tests belonged to the class with null hypothesis of no cointegration and 8 tests belonged to the class with null hypothesis ii of cointegration. These tests were evaluated on basis of stringency criterion (Zaman 1996) using Monte Carlo methods and on basis of Empirical Size and Power properties using real data of income and consumption of 100 countries from 1970 to 2014. First tests were assessed for their size stability using asymptotic critical values and then size of these tests was stabilized using simulated critical values. All of 24 tests were assessed on basis of stringency criterion using simulated critical values by considering two assumptions, one that the nature of deterministic part was known a priori and second that the nature of deterministic part was unknown, so an automatic selection procedure of deterministic part (Elder and Kennedy 2001) was followed. As it is strongly recommended by economic theory that income and consumption of same country are cointegrated and income and consumption of two different countries are not cointegrated, so on basis of this economic theory all of 24 tests were evaluated for their performance on basis of Empirical Size and Power. The study revealed that almost all of tests i.e. 21 out 24 had unstable size when asymptotic critical values were used and all of tests had stable size when simulated critical values were used. From tests with null hypothesis of no cointegration, Phillips and Ouliaris ˆ P m test was the leading performer as it was the most stringent test at all specifications of deterministic part. In addition to this test Choi Durbin-Hausman test, Phillips and Ouliaris’ ˆt Z test, Phillips and Ouliaris’ ˆ Z a test and the t-test of cointegration in a single equation Error Correction Model also performed overall better than rest of tests. Three tests i.e. Johansen Trace, Johansen Maximum Eigen Value and Phillips and Ouliaris ˆz P performed overall poor. In same manner from tests with null hypothesis of cointegration LM test based on KPSS statistic was the leading performer as it was the iii most stringent test for all of three specifications of deterministic part. Shin’s C test was the second overall better performing test and three tests i.e. Xiao Fluctuation, Hansen’s cL and Hausman H1 overall performed poorly. It was also revealed that on basis of real data comparison tests with null hypothesis of no cointegration performed way well as compared to tests with null hypothesis of cointegration. On basis of real data, from tests with null hypothesis of no cointegration five tests i.e. the t-test of cointegration in a single equation Error Correction Model, Boswijk Wald test, Phillips and Ouliaris ˆ P m test, Phillips and Ouliaris’ ˆ Z a test and Choi Durbin-Hausman test were the leading performers both in terms of Empirical Size and Power. However, on basis of real data, from test with null hypothesis of cointegration not a single test had performance that is worth to mention. It is recommended in light of our study that use of asymptotic critical values may be eluded and instead simulated critical values may be used. It is also recommended that tests with null hypothesis of no cointegration may be given preference over tests with null hypothesis of cointegration for a specific real economic problem and if tests with null hypothesis of cointegration are used then, they may only be used for confirmatory purposes. Five tests with null hypothesis of no cointegration are recommended and these are Phillips and Ouliaris ˆ P m test, the t-test of cointegration in a single equation Error Correction Model, Boswijk Wald test, Phillips and Ouliaris’ ˆ Z a test and Choi Durbin-Hausman test. Similarly, from tests with null hypothesis of cointegration only one test i.e. LM test based on KPSS statistic may be used for confirmatory purpose.