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Home > Analysis of Medicinal and Chemical Properties of Honeys Collected from Some Selected Locations of Pakistan

Analysis of Medicinal and Chemical Properties of Honeys Collected from Some Selected Locations of Pakistan

Thesis Info

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Author

Noor, Nadia

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7010/1/Nadia_Noor_Chemistry_UAF_2015.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725485311

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Nature has blessed us with large number of food stuffs honey is one of them. It is composed of variety of substances mainly sugars, moisture and bioactive constituents. Its composition depends on geographical and botanical origin. Physicochemical analysis is a tool for quality evaluation, detection of adulteration and geographical discrimination of honey. Honey is also reported to exhibit excellent bioactive composition which is responsible for therapeutic behavior of this valued natural product. Therefore, present study was designed to evaluate physicochemical properties, bioactive composition, antioxidant profile, antimicrobial behavior and antitumor potential of honey. Honey was also used as bio indicator to detect the heavy metal toxicity of some areas of Pakistan. Natural honey samples were collected from some selected locations of Punjab, Pakistan. Beekeeper’s and branded honey samples were collected from local markets of Pakistan. Quantitative, qualitative and medicinal properties of honey samples were evaluated by using spectroscopic and chromatographic techniques. Nutritional values of all Pakistani honey samples assessed by physicochemical analysis helped to conclude that all of our honey samples obeyed international standards. EC values of beekeeper’s honey samples were close to those found for natural Pakistani honeys. Natural and commercial Pakistani honeys showed higher protein content as compared to available international data. Proline is the major amino acid of honey. Natural Pakistani honey samples were excellent as compared to branded and beekeeper’s honey samples. HPLC analysis of fructose and glucose ratio and GCMS analysis of isotopic ratio analysis of C13/C12 highlighted the adulteration in commercial and branded honey samples. Trace metal concentration (Ca, Mg, Fe, Mn and Zn) of all analyzed honey samples were also comparable with available data. In case of toxic metals many regions like Faisalabad, Multan, Bahawalpur, Abdulhakeem and Dunyapur were found to contain toxic metals more than permissible levels; lead and chromium being more prominent. Almost no metal toxicity was found in commercial honeys. Total phenolic, total flavonoids, ascorbic acid, beta carotenoids and lycopene were quantitatively found in natural honey samples in much higher amount as compared commercial honey samples which contained in low amount. All studied honey samples have shown excellent DPPH radical scavenging power, ferric reducing antioxidant power and peroxide scavenging activities. Natural Pakistani honeys produced zones of inhibition in the range of 10-28 mm for all tested bacterial strains whereas antifungal potential observed against all analyzed strains was very poor. Many Pakistani honeys have excellent antitumor potential as compared to many plant extracts. Some natural samples have antitumor activity close to the standard and even two samples have shown more than standard. Many commercial honey samples also have appreciable antitumor activities. Overall Pakistani honeys were found to be promising natural products with excellent nutritional and medicinal properties.
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یحییٰ اعظمی

یحییٰ اعظمی
ناظرین کو یہ معلوم کر کے افسوس ہوگا کہ دفتر دارالمصنفین کے منصرم یحییٰ صاحب اعظمی نے چند دنوں کی علالت کے بعد ۲۲؍ فروری کو انتقال کیا، وہ عمر بھر دارالمصنفین سے وابستہ رہے اور مرکر اس سے جدا ہوئے، مرحوم بڑے متدین اور دفتری کاموں میں تجربہ کار تھے، ہزاروں روپیے کا کاروبار ان کے ہاتھ میں تھا اور کبھی ایک حبہ کا فرق نہیں نکلتا تھا، ایسے قابل اعتماد آدمی مشکل سے ملیں گے، طبیعت میں حد سے زیادہ نظافت و نفاست تھی، بڑی صاف ستھری زندگی بسر کرتے تھے، ان کا دفتری کام بھی بڑا صاف ستھرا تھا، خشک دفتری کاموں کے ساتھ خوشگو شاعر بھی تھے، ان کے کلام کے دو مجموعے ’’نوائے حیات‘‘ اور ’’نوائے عصر‘‘ شائع ہوچکے ہیں، اﷲ تعالیٰ ان کی مغفرت فرمائے۔
(شاہ معین الدین ندوی، مارچ ۱۹۷۲ء)

Analysis of Drivers’ Characteristics Concerning Speeding Behavior and Crash Involvement in Oman

This study aims to identify the significance of driver’s socioeconomic demographics (SEDs) in the decision to speed and crash involvement. A questionnaire was designed consisting of a driver’s SEDs, speeding propensity, and crash experience. This questionnaire was conducted with the students and employees of the University of Nizwa and other drivers at the selected locations. A total of 604 usable samples were obtained. Simple frequency distribution and discriminant multivariate analysis were conducted on the driver’s responses. Survey results revealed that about 47.7% of the drivers have experienced a crash. The driver’s gender nationality, profession, age, type of vehicle drive, driving experience, and past crash experience are significant attributes of the driver’s speeding behavior. Ordered probit analysis for speeding behavior and simple probit regression analysis for crash involvement was conducted. The male drivers and those who are under the age of 30 years and have driving experience of more than 3 years have more likelihood to exceed the speed limits than other drivers. Similarly, the driver’s gender, age (≤ 30 years), and those who are employees have a significant correlation with the propensity of crash involvement. Male and young drivers have more likelihood to be involved in a crash.

Microbial Dynamics in Salt Affected Soils

Soil salinity is a worldwide problem which not only influences the physical and chemical properties of soil but may also seriously affect the microbiological properties of soil. This project was undertaken to assess the behavior of various microbiological properties of soil in relation to salinity in a series of incubation and pot experiments during 2009-12. Initially the soil microbiological properties of thirty naturally occurring diverse salt affected soils were determined. Based on the results of preliminary experiments, further experiments were conducted to evaluate the effect of NaCl and MgCl 2 induced salinity on N dynamics and soil microbial activity in soil. Finally, a pot experiment was conducted to assess the effect of important amendments such as pressmud, gypsum and farmyard manure, which are normally used for the reclamation of salt affected soils, on growth of wheat and maize and on various microbial attributes in a highly saline-sodic soil (EC 20.3 dS m -1 ). In addition, some bacteria and fungi were isolated from a highly saline-sodic soil, and the effects of same organisms were also tested in the above experiment. For experiment 1, soil samples at 0-20 cm were collected from various salt affected soils ranged in salinity from EC < 4.0 to 32 dS m -1 in November 2009. The samples were analyzed for soil microbiological (such as microbial biomass-C, microbial biomass-N, N mineralization, nitrification, rate of soil respiration) and chemical properties (such as pH, EC, soluble Ca+Mg, soluble Na, SAR (sodium adsorption ratio), ESP (exchangeable sodium percentage), carbonates, bicarbonates, chloride). The results showed that almost all microbial indices including microbial biomass-C (MBC), microbial biomass-N (MBN), basal soil respiration, nitrification and net N mineralization were negatively correlated with increasing salinity (r = -0.89, -0.74, - 0.79, -0.64 and -0.57 respectively). The results further showed that the depressive effects of SAR and ESP on various soil microbial indices were much stronger than that of carbonates and bicarbonates. Both NaCl and MgCl 2 induced salinity significantly reduced the rate of CO 2 evolution and N mineralization during all xiiiincubation periods. The depressive effects increased with increase in salts level. The NaCl induced salinity depressed cumulative CO 2 production by about 80% and N mineralization by 50% during 40 days of incubation. Whereas MgCl 2 induced salinity decreased cumulative CO 2 production by 95% and N mineralization by 81% during 30 days of incubation. These results suggested that the impact of MgCl 2 salinity on microbial indices was stronger than that of NaCl. The amendment of saline-sodic soil with pressmud, gypsum and farmyard manure (FYM) significantly improved the rate of CO 2 evolution, N mineralization, microbial biomass-C and microbial biomass-N. The effects were more pronounced with combined application of pressmud with farmyard manure or gypsum. It was also evident that the effect of pressmud + FYM was further improved with microbial inoculation of microbial isolation from highly salt affected soil. Organic matter decomposition generally increases the production of CO 2 and liberation of H + ions. The H + ion enhance the dissolution of CaCO 3 and releases more Ca to replace Na from clay particles and thus helps in the reclamation of saline-sodic soils. Both wheat and maize seeds failed to germinate under the prevailing saline-sodic conditions despite repeated re-seeding and thus no data was generated in this respect. These results suggested that all microbial indices decreased significantly with increasing salinity levels indicating that soil microorganisms were sensitive to different types of salinity. Thus, salinity is a stress factor and can reduce microbial diversity and control microbial abundance, composition and functions. Although, amendments of saline-sodic soil with organic sources (such as FYM, pressmud) substantially improved microbial attributes they did not enable the germination and survival of wheat and maize sown in the soil. Nevertheless the evidence suggests that organic matter may play significant role in the amelioration of saline-sodic or sodic soils.