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Home > Analysis of Nacl Tolerance in Calli and Regerants of Unadapted and Licl Adapted Lines of Rice Oryza Sativa L.

Analysis of Nacl Tolerance in Calli and Regerants of Unadapted and Licl Adapted Lines of Rice Oryza Sativa L.

Thesis Info

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External Link

Author

Khalid, Qudsia

Program

PhD

Institute

The University of Agriculture

City

Peshawar

Province

KPK

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Agricultural Technology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/10831/1/Qudsia%20Khalid_Plant%20Biotech_2017_AUP_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725488112

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In this study the response of calli lines of Oryza sativa L. cv.Swat-1 adapted to ion specific stress (25mM LiCl) was investigated to elucidate the underlying mechanisms of adaptations at the cellular level and their stability at regenerant stage. The physiological and biochemical parameters revealed that adaptation caused a decrease in proline, Na+, Na+/K+ratio, protein content and regeneration capability, while K+,Ca++and Mg++concentration enhanced. Exposure of cell lines to interactive effect of NaCl and CaCl2 showed that high calcium reduced the Na+ toxic effect on relative growth rates of both the cell lines with a concomitant increase in K+, Ca++ and Mg++ content respectively. While total sugar and proline contents of unadapted line increased and of adapted line decreased. Adaptation caused the appearance of one new protein band of about 67 kDa which remained stable under 200mM NaCl stress. While this band slightly appeared in unadapted line with degradation of other low molecular weight protein e.g. 17, 19 and 20 kDa. High calcium chloride caused upregulation of 67 kDa band with restoration of the damaged proteins except 17 kDa band. Regenerants and plantlets of adapted line showed excessive accumulation of osmolytes (sugar, proline) compared to accumulation in unadapted line. On the other hand the proteins’ banding pattern of regenerants was found to be similar to their respective lines. Interestingly 67 kDa band disappeared from the shoot of regenerated plantlets but was present in roots. No polymorphism was detected when 17 SSR markers related to Saltol region , 6 SSR markers for Na+/K+ ratios and one each for Na+/H+ antiport K+/Na+ symporter activity at plasma membrane was used. Transcriptional and translational inhibitors studies showed that changes due to adaptation were at translational/software level. This study reveals that the adaptation to ionic stress and supplemental CaCl2 under NaCl stress works on a similar principal of cellular homeostasis regain and adaptation at the cellular level is transferable (stable) to complete plant level.
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محمد خالد

                یوسف نیر(۱۹۴۷ء ۔۲۰۱۷) کا اصل نا م یوسف رحمت ہے اور نیر تخلص ہے ۔ آپ محلہ اٹاری گیٹ سیالکوٹ میں پیدا ہوئے۔(۱۰۹۰) آپ نے ایم ۔اے اردو پنجاب یونیورسٹی اور ایم۔فل اردو علامہ اقبال اوپن یونیورسٹی سے کیا۔ ۱۹۷۹ء میں آپ کی تعیناتی بطور لیکچرار گورنمنٹ کالج رحیم یار خان میں ہوئی۔ گورنمنٹ کالج سیالکوٹ سے ایسوسی ایٹ پروفیسر کے عہدے پر آپ ریٹائر ہوئے ۔(۱۰۹۱) آپ کالج میگزین lکے مدیر رہے اور ان کی ادبی تخلیقات اس میں شائع ہوتی تھیں۔ آپ کا شعری کلام ’’کاتھولک‘‘،’’نقیب‘‘ ،لاہور ،’’شاداب ‘‘ لاہور ، ’’شعاعِ نور‘‘ ،لاہور ،’’بیسویں صدی‘‘ ،لاہور اور ’’فنون ‘‘ لاہور میں شائع ہوتا رہا۔

                آپ مرے کالج کی مجلسِ سخن اور مجلسِ اقبال کے انچارج رہے۔ نیرمرے کالج کے علمی و ادبی مجلہ ’’الفیض ‘‘ کے نگران رہے اور مرے کالج سے اقبال نمبر اور غالب نمبر شائع کیے۔۱۹۸۸ء میں آپ پاکستان رائٹرز گلڈ کے مرکزی صدر منتخب ہوئے۔ پنجابی ادب سنگت لندن نے انھیں ۲۰۰۰ء کاادبی ایوارڈ لندن میں ایک مشاعر ے میں پیش کیا۔ (۱۰۹۲)’’روشنی کا پہلا دن‘‘ یوسف نیر کا شعری مجموعہ الحمد پبلی کیشنز نے ۱۹۹۲ء کو شائع کیا۔

                یوسف نیر ادب میں ادب برائے زندگی نظریے کے قائل ہیں۔ انھوں نے اپنی شاعری میں ہمیشہ غریب ،مظلوم اور پسماندہ معاشرے کے پسے ہوئے انسانوں کے دکھ اور محرومی کی بات کی ہے۔ وہ ظالم ،جابر اور استحصالی نظام اور افراد کی مـذمت کرتے ہیں۔ نیر گہرا سماجی شعور رکھتے ہیں وہ سماجی اور معاشرتی ظلم و ستم کو نظر انداز نہیں کرتے بلکہ اسے محسوس کرتے ہیں اور اپنی شاعری میں جا بجا بیان کرتے نظر آتے ہیں:

راہ کوئی نہیں ہے بچنے کی……

 

1ہر طرف شیش ناگ بیٹھے ہیں

 

EFFECTS OF MULLIGAN ROTATIONAL MOVEMENT VERSUS MEDIAL GAPPING TECHNIQUE ON PAIN, RANGE OF MOTION AND DISABILITY IN PATIENTS WITH KNEE OSTEOARTHRITIS

Background and Aim: To compare the effects of mulligan rotational movement and medial gapping technique on pain, range of motion and disability in knee osteoarthritis patients. Methodology: This study was a Randomized Clinical Trial. The data was collected using a convenience sampling technique. Data was collected from Jinnah Hospital Lahore, from 15th December – 30th June 2022.36 subjects (males and females) were recruited in two groups. The first group received Mobilization with movement along with a conservative treatment protocol. The second group received the Medial gapping technique along with conservative treatment protocol. Each group was treated for four weeks in which three sessions per week were given. Numeric pain rating scale and the disability index were used as outcome measures. Data was analyzed through statistical package for the social sciences (SPSS) version 25. Results: Within-group comparison by paired t-test showed the p-value was significant <.05, indicating that both treatment was effective in improving symptoms. Between groups, comparison by independent t-test showed that Mulligan Mobilization Technique is more effective than the medial gapping technique in improving pain disability and quality of life. Conclusion: The study concluded that Mulligan Mobilization Technique provides more clinical benefits regarding pain, disability and range of motion in osteoarthritis patients than Medial Gapping Technique.

Privatization of Agricultural Extension System in the Punjab, Pakistan: A Swot Analysis

Globally, there is a trend towards privatization due to poor performance of public extension services. Private agricultural extension system is considered demand-driven, cost-effective with efficient and quality service. In Pakistan, Privatization of agricultural extension system was started in 1988 when the then Government of Pakistan established a commission to look into the causes of poor performance of agricultural sector and suggest ways to improve its performance. The commission suggested the involvement of the private sector in reshaping agricultural extension. It was a major policy shift, in which the supply of inputs was shifted to private sector from public sector. Currently, 320 pesticide companies (private sector) are working in the Punjab. These companies not only provide pesticide products to the farmers through dealers but also provide advisory services to them. Privatization experiences in the world had mixed results. In some cases it has positive results while in other location the results are discouraging. The present study was conducted to analyze the strengths, weaknesses, opportunities and threats for private extension system in the Pakistani context where the literature is either silent or scanty about the performance of private sector extension. A cross-sectional research design was used for the study. The study was carried out in the Punjab province which comprises five cropping zones, out of which, three zones were selected purposively. Multistage proportionate sampling technique was used for the selection of respondents. A sample of 408 respondents was selected (136 from each zone) by using simple random sampling technique. Sample size was determined by using Fitzgibbon table (Fitzgibbon & Lynn, 1987). Similarly, a sample of 60 respondents from EFS of Syngenta (Pesticide Company) were also selected randomly by using the same table. The data were collected through personal interviews with the help of validated research instrument. The data, thus collected were analyzed by using computer software (i.e. SPSS). The results showed that a diagnostic skill of the EFS was strength of the system. Attributes of the EFS i.e. politeness and good conduct with farmers were also rated as strengths. Group discussion method was rated as strength of the system. However, subject matter coverage, target beneficiaries, extension approach and functions, communication, competencies of EFS of private extension found to be the weakness of the system. Limited opportunities existed for the demand of advisory services for specific crops at specific location and fee-based private extension system in the country. Overall it poses threat for the system. There were great discrepancies observed between the perceptions of the farmers and the EFS about the same questions. According to the EFS, the system has more strengths as compared to weaknesses. It is suggested that the steps should be taken to mitigate the weaknesses and ensure the strengths of the system. Similarly, threats should be converted into opportunities.