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Antibiotic Residue in Poultry Products

Thesis Info

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Author

Farah Asad

Program

PhD

Institute

University of Agriculture

City

Faisalabad

Province

Punjab

Country

Pakistan

Thesis Completing Year

2012

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/381

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725505504

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This study was conducted for the detection and evaluation of antibiotic residues in poultry products. This research work completed in three different phases. In the first-phase sample, survey was done in different farm houses in and around Faisalabad, and antibiotic residues were detected by microbiological assay. In the second phase, withdrawal period of fluoroquinolone antibiotics was investigated in experimental birds. Samples of liver and muscles of these experimental birds were cooked by electric and microwave ovens. All samples (serum, muscle, liver, kidney and eggs) were extracted for fluoroquinolones and quantified by HPLC with fluorescent detection. Health biomarkers of all samples were analyzed by their reference methods. Percentage of positive samples for antibiotic residues was calculated during a survey. This indicated the wide-spread use of antibiotics in most farm houses and high residue persistence in liver, heart and lung tissues. Seasonal variations were also investigated, and residue prevalence was observed in rainy season. In phase 2nd withdrawal time study, the mean concentrations (mean±SE) of fluoroquinolones were calculated. Analysis of variance and Duncan multiple range test was applied. Significant differences were observed in concentrations (in serum, muscle, liver and kidney) of fluoroquinolones in different days after slaughter. The concentrations were significantly high at day 01 and then decreased and disappeared at day 03 and day 04, respectively. Fluoroquinolones level also depleted after cooking of meat. Health biomarkers were significantly affected by fluoroquinolones in treated birds. Deposition of these antibiotic residues in edible tissues causes many hazards to human as well as animals. In phase III of this experiment, muscles and liver that were positive for their residues during different days of the washout time were subjected to two different cooking methods i. e. by electric oven and by microwave oven. On each experimental day, muscle and liver residues, concentration did decrease significantly with microwave and electric oven cooked samples, and were below the maximum residue limit (MRL) value. Left over residue in meat below MRL’ s value cause allergic or anaphylactic reaction, toxicity, killing of beneficial bacteria in animal and human intestine and occurrence of resistant strains of bacteria in human. In addition aftermath of this will affect economy as well as health of the individuals.
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حضورؐ آپ نے ریگِ تپاں کو لعل کیا


ؑحضورؐ! آپؐ نے ریگِ تپاں کو لعل کیا
جہانِ دشتِ کو یوں شہرِ بے مثال کیا

رفیقِ خاص کو صدیقؓ کا لقب بخشا
وہ ترجمان تھے ماضی کے ، شانِ حال کیا

جسے تھی اونٹ چرانے میں دقّتیں بے حد
دعا کے ساتھ اُسے صاحبِ کمال کیا

غنیؓ کی دولتِ دنیا کو حسنِ دیں بخشا
حیا کے پیکرِ احسن کو خوش خصال کیا

لعابِ پاک کا فیضانِ خاص تھا جس نے
علیؑ کو شوکتِ خیبر سے مالامال کیا

مقامِ احسنِ تقویم پایا ، اسفل نے
جہاں میں منصبِ انسانیت بحال کیا

تمام روئے زمیں سجدہ گاہ کر ڈالی
خدا نے اُمتِ محبوبؐ کا خیال کیا

رہِ حیات میں جب بھی چلے ہیں تیرِ الم
تو یادِ مونسِ جاںؐ کو غموں میں ڈھال کیا

یہ نعتِ پاک کا عرفانؔ! فیض ہے جس نے
ہر ایک حرفِ سخن تیرا لازوال کیا

An Analysis of Indo-Pakistan Nuclear Doctrines

This study explores the dynamics of nuclear politics in Indo-Pakistan relations. The events after the nuclearization of South Asia aroused an extensive discussion about the basic reasons of countries becoming nuclear. Deterrence idealists have put forward the Nuclear Peace Theory advocating that nuclear arms make war terribly expensive and evade conflict among atomic opponents and thus generate constancy among them. Deterrence cynics have debated that the new nuclear-powered countries would not be able to accomplish the rudimentary requisites for deterrence stability as they would be disposed to defensive and preventive war tactics, construct susceptible atomic weaponries that would disposed to illicit usage. While discussing both stability and instability of nuclear weapons, the intellectuals disregard the importance of nuclear doctrines of the nuclear states which can cause deterrence disappointment or guaranteeing its strength. The author has used primary and secondary data. The primary sources are news bulletins and websites and secondary sources are books, journals etc.

An Evaluation of Early Childhood Special Education Program of Government Deaf & Defective Hearing School in Punjab

The main purpose of this study was to evaluate Early Childhood Special Education (ECSE) program of Government Deaf & Defective Hearing Schools (GDDHS) in Punjab. To serve this purpose, I conducted an in depth review of literature. Robert Stake‟s Countenance Model, being more suitable for the evaluation of educational programs was selected. According to this model, the ECSE program was evaluated following antecedents---transactions----outcomes pattern. The population of study included principals, special education teachers, young children with deafness, and their parents from all 34 Government Deaf & Defective Hearing Schools working in 31 districts of the Punjab province. A representative sample of principals, special education teachers, young children with deafness (YCWD), and their parents was taken according to the requirements of the study. Depending on the nature, and keeping the objectives and questions of the study in view, certain questionnaires, structured interviews, and achievement tests were prepared. To evaluate the ECSE program for young children with deafness at the antecedents phase, two instruments including: demographic information sheet about young children with deafness, and a structured interview schedule for principals were developed. Curriculum being taught to young children with deafness in GDDHS was also reviewed. At the transaction phase, three instruments including two questionnaires on instructional practices of special education teachers, and their opinions about parental involvement in the education of their young children with deafness, and one structured interview schedule for parents to know their satisfaction on and involvement in the provision of early childhood special education to their young children with deafness were developed. At the outcome phase, eight achievement tests on writing (Urdu & English), mathematics, reading recognition (Urdu & English), speech reading (Urdu & English), and speech were prepared. I collected all data personally. Analysis and interpretation of the collected data were performed in chapter IV applying descriptive and statistical techniques including frequency distribution of responses, t-test, and ANOVA. Qualitative data including review of ECSE curriculum, and interviews were analyzed using narrative technique, and transcribing, coding, and deriving themes which were, subsequently, analyzed on SPSS. The major findings revealed that ECSE program was not fulfilling its objectives of mobilizing parents, professionals, community, and other stakeholders for early detection and intervention of young children with deafness, providing ECSE to all children with deafness from 3-8 years old, teaching Urdu, English, and mathematics to make them functionally literate, preparing them for inclusive education through developing their speech, speech reading, language, communication, and auditory skills. Curriculum for ECSE was not up to the standard. No significant difference was found among instructional practices adopted by special education teachers. Their opinions about parental involvement in the education of their young children with deafness did not show significant difference except on the basis of their academic qualifications. Interviews with parents reflected their less satisfaction on and less involvement in the provision of ECSE to their young children with deafness. The results of achievement tests showed a significant difference in the performance of children with deafness in a deaf school located in Zone IV as compared to other three zones. The reasons included the excessive number of young children with deafness in classes, their late admissions, most of the children having congenital deafness and profound degree of hearing loss, unavailability of hearing aids, lack of additional coaching at home, most of the parents with poor socioeconomic status, and having more than one child with deafness, no plan of action for mobilization of parents, community, and other stake holders, lack of teacher training courses on ECSE, absence of the component of parental training, unavailability of text and work books, lapses in the implementation of inclusive education project, lack of teaching staff in schools, and deficiencies in the ECSE curriculum. All these were proving obstacles in achieving the objectives of ECSE program. On the basis of major findings it was recommended that to maintain quality, restricted number of young children with deafness should be kept in one class. Early detection and intervention, and admissions of children in schools at an early age should be ensured through media campaigns, pamphlets, banners, and visits to remote areas. The parents should be involved in the educational planning and decision making related to the placement of their children in future. Inter family marriages should be avoided. Hearing aids should be provided to all young children with deafness, and their regular use and maintenance should be ensured through developing a monitoring system. Training programs for teachers and parents should be arranged on regular basis. The serious issues of unavailability of text and work books, unimplementation of inclusive education plans, and development of curriculum of K.G.I and K.G.II should be resolved on emergent basis. The instructional practices adopted by the special education teachers should be improved and monitored.